Abstract
Five monoclonal antibodies specific for bovine respiratory syncytial virus were characterized by Western immunoblotting, radioimmunoprecipitation, and epitope mapping assays. The monoclonal antibodies were found to be specific for the fusion protein, and there were at least two antigen binding sites, one of which was neutralizing.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson L. J., Hierholzer J. C., Stone Y. O., Tsou C., Fernie B. F. Identification of epitopes on respiratory syncytial virus proteins by competitive binding immunoassay. J Clin Microbiol. 1986 Mar;23(3):475–480. doi: 10.1128/jcm.23.3.475-480.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baker J. C., Frey M. L. Bovine respiratory syncytial virus. Vet Clin North Am Food Anim Pract. 1985 Jul;1(2):259–275. [PubMed] [Google Scholar]
- Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
- Collins J. K., Teegarden R. M., MacVean D. W., Salman, Smith G. H., Frank G. R. Prevalence and specificity of antibodies to bovine respiratory syncytial virus in sera from feedlot and range cattle. Am J Vet Res. 1988 Aug;49(8):1316–1319. [PubMed] [Google Scholar]
- Klucas C. A., Anderson G. A. Bovine respiratory syncytial virus-specific monoclonal antibodies. Vet Immunol Immunopathol. 1988 Jun;18(4):307–315. doi: 10.1016/0165-2427(88)90158-4. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Walsh E. E., Brandriss M. W., Schlesinger J. J. Purification and characterization of the respiratory syncytial virus fusion protein. J Gen Virol. 1985 Mar;66(Pt 3):409–415. doi: 10.1099/0022-1317-66-3-409. [DOI] [PubMed] [Google Scholar]
- Walsh E. E., Cote P. J., Fernie B. F., Schlesinger J. J., Brandriss M. W. Analysis of the respiratory syncytial virus fusion protein using monoclonal and polyclonal antibodies. J Gen Virol. 1986 Mar;67(Pt 3):505–513. doi: 10.1099/0022-1317-67-3-505. [DOI] [PubMed] [Google Scholar]
- Walsh E. E., Schlesinger J. J., Brandriss M. W. Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus. J Gen Virol. 1984 Apr;65(Pt 4):761–767. doi: 10.1099/0022-1317-65-4-761. [DOI] [PubMed] [Google Scholar]