Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Sep;29(9):2051–2055. doi: 10.1128/jcm.29.9.2051-2055.1991

Reactivity of monoclonal antibodies to the 41-kilodalton protein of porcine group C rotavirus with homologous and heterologous rotavirus serogroups in immunofluorescence tests.

C K Ojeh 1, B M Jiang 1, H Tsunemitsu 1, S Y Kang 1, P A Weilnau 1, L J Saif 1
PMCID: PMC270259  PMID: 1663522

Abstract

Three monoclonal antibodies (MAbs) to porcine group C rotavirus immunoprecipitated the major inner capsid protein (41 kDa) but failed to precipitate group A rotavirus proteins. In immunofluorescence tests of rotavirus-infected cell cultures or pig intestines, the MAbs recognized porcine and bovine group C rotaviruses but not group A or B rotaviruses. These MAbs may recognize the group C rotavirus counterpart to VP6 of group A rotaviruses and may be useful as diagnostic reagents.

Full text

PDF
2051

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bohl E. H., Saif L. J., Theil K. W., Agnes A. G., Cross R. F. Porcine pararotavirus: detection, differentiation from rotavirus, and pathogenesis in gnotobiotic pigs. J Clin Microbiol. 1982 Feb;15(2):312–319. doi: 10.1128/jcm.15.2.312-319.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bohl E. H., Theil K. W., Saif L. J. Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. J Clin Microbiol. 1984 Feb;19(2):105–111. doi: 10.1128/jcm.19.2.105-111.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bremont M., Cohen J., McCrae M. A. Analysis of the structural polypeptides of a porcine group C rotavirus. J Virol. 1988 Jun;62(6):2183–2185. doi: 10.1128/jvi.62.6.2183-2185.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bridger J. C., Brown J. F. Prevalence of antibody to typical and atypical rotaviruses in pigs. Vet Rec. 1985 Jan 12;116(2):50–50. doi: 10.1136/vr.116.2.50. [DOI] [PubMed] [Google Scholar]
  5. Bridger J. C. Detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the faeces of piglets with diarrhoea. Vet Rec. 1980 Dec 6;107(23):532–533. [PubMed] [Google Scholar]
  6. Burns J. W., Welch S. K., Nakata S., Estes M. K. Characterization of monoclonal antibodies to human group B rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. J Clin Microbiol. 1989 Feb;27(2):245–250. doi: 10.1128/jcm.27.2.245-250.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Caul E. O., Ashley C. R., Darville J. M., Bridger J. C. Group C rotavirus associated with fatal enteritis in a family outbreak. J Med Virol. 1990 Mar;30(3):201–205. doi: 10.1002/jmv.1890300311. [DOI] [PubMed] [Google Scholar]
  8. Chasey D., Davies P. Atypical rotaviruses in pigs and cattle. Vet Rec. 1984 Jan 7;114(1):16–17. doi: 10.1136/vr.114.1.16. [DOI] [PubMed] [Google Scholar]
  9. Dimitrov D. H., Estes M. K., Rangelova S. M., Shindarov L. M., Melnick J. L., Graham D. Y. Detection of antigenically distinct rotaviruses from infants. Infect Immun. 1983 Aug;41(2):523–526. doi: 10.1128/iai.41.2.523-526.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Espejo R. T., Puerto F., Soler C., González N. Characterization of a human pararotavirus. Infect Immun. 1984 Apr;44(1):112–116. doi: 10.1128/iai.44.1.112-116.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Greenberg H., McAuliffe V., Valdesuso J., Wyatt R., Flores J., Kalica A., Hoshino Y., Singh N. Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infect Immun. 1983 Jan;39(1):91–99. doi: 10.1128/iai.39.1.91-99.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hung T., Chen G. M., Wang C. G., Yao H. L., Fang Z. Y., Chao T. X., Chou Z. Y., Ye W., Chang X. J., Den S. S. Waterborne outbreak of rotavirus diarrhoea in adults in China caused by a novel rotavirus. Lancet. 1984 May 26;1(8387):1139–1142. [PubMed] [Google Scholar]
  14. Jashés M., Sandino A. M., Faúndez G., Avendaño L. F., Spencer E. In vitro transcription of human pararotavirus. J Virol. 1986 Jan;57(1):183–190. doi: 10.1128/jvi.57.1.183-190.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jiang B. M., Saif L. J., Kang S. Y., Kim J. H. Biochemical characterization of the structural and nonstructural polypeptides of a porcine group C rotavirus. J Virol. 1990 Jul;64(7):3171–3178. doi: 10.1128/jvi.64.7.3171-3178.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kang S. Y., Saif L. J., Miller K. L. Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses. J Clin Microbiol. 1989 Dec;27(12):2744–2750. doi: 10.1128/jcm.27.12.2744-2750.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Matsumoto K., Hatano M., Kobayashi K., Hasegawa A., Yamazaki S., Nakata S., Chiba S., Kimura Y. An outbreak of gastroenteritis associated with acute rotaviral infection in schoolchildren. J Infect Dis. 1989 Oct;160(4):611–615. doi: 10.1093/infdis/160.4.611. [DOI] [PubMed] [Google Scholar]
  19. McNulty M. S., Allan G. M., Todd D., McFerran J. B., McCracken R. M. Isolation from chickens of a rotavirus lacking the rotavirus group antigen. J Gen Virol. 1981 Aug;55(Pt 2):405–413. doi: 10.1099/0022-1317-55-2-405. [DOI] [PubMed] [Google Scholar]
  20. Nagesha H. S., Hum C. P., Bridger J. C., Holmes I. H. Atypical rotaviruses in Australian pigs. Arch Virol. 1988;102(1-2):91–98. doi: 10.1007/BF01315565. [DOI] [PubMed] [Google Scholar]
  21. Pedley S., Bridger J. C., Brown J. F., McCrae M. A. Molecular characterization of rotaviruses with distinct group antigens. J Gen Virol. 1983 Oct;64(Pt 10):2093–2101. doi: 10.1099/0022-1317-64-10-2093. [DOI] [PubMed] [Google Scholar]
  22. Pedley S., Bridger J. C., Chasey D., McCrae M. A. Definition of two new groups of atypical rotaviruses. J Gen Virol. 1986 Jan;67(Pt 1):131–137. doi: 10.1099/0022-1317-67-1-131. [DOI] [PubMed] [Google Scholar]
  23. Rodger S. M., Bishop R. F., Holmes I. H. Detection of a rotavirus-like agent associated with diarrhea in an infant. J Clin Microbiol. 1982 Oct;16(4):724–726. doi: 10.1128/jcm.16.4.724-726.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Saif L. J., Bohl E. H., Theil K. W., Cross R. F., House J. A. Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. J Clin Microbiol. 1980 Jul;12(1):105–111. doi: 10.1128/jcm.12.1.105-111.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Saif L. J., Terrett L. A., Miller K. L., Cross R. F. Serial propagation of porcine group C rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus. J Clin Microbiol. 1988 Jul;26(7):1277–1282. doi: 10.1128/jcm.26.7.1277-1282.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Singh N., Sereno M. M., Flores J., Kapikian A. Z. Monoclonal antibodies to subgroup 1 rotavirus. Infect Immun. 1983 Nov;42(2):835–837. doi: 10.1128/iai.42.2.835-837.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Taniguchi K., Urasawa T., Urasawa S., Yasuhara T. Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination. J Med Virol. 1984;14(2):115–125. doi: 10.1002/jmv.1890140205. [DOI] [PubMed] [Google Scholar]
  28. Terrett L. A., Saif L. J. Serial propagation of porcine group C rotavirus (pararotavirus) in primary porcine kidney cell cultures. J Clin Microbiol. 1987 Jul;25(7):1316–1319. doi: 10.1128/jcm.25.7.1316-1319.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Terrett L. A., Saif L. J., Theil K. W., Kohler E. M. Physicochemical characterization of porcine pararotavirus and detection of virus and viral antibodies using cell culture immunofluorescence. J Clin Microbiol. 1987 Feb;25(2):268–272. doi: 10.1128/jcm.25.2.268-272.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Theil K. W., Saif L. J. In vitro detection of porcine rotavirus-like virus (group B rotavirus) and its antibody. J Clin Microbiol. 1985 May;21(5):844–846. doi: 10.1128/jcm.21.5.844-846.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Theil K. W., Saif L. J., Moorhead P. D., Whitmoyer R. E. Porcine rotavirus-like virus (group B rotavirus): characterization and pathogenicity for gnotobiotic pigs. J Clin Microbiol. 1985 Mar;21(3):340–345. doi: 10.1128/jcm.21.3.340-345.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Ushijima H., Honma H., Mukoyama A., Shinozaki T., Fujita Y., Kobayashi M., Ohseto M., Morikawa S., Kitamura T. Detection of group C rotaviruses in Tokyo. J Med Virol. 1989 Apr;27(4):299–303. doi: 10.1002/jmv.1890270408. [DOI] [PubMed] [Google Scholar]
  33. Welch S. K., Saif L. J. Monoclonal antibodies to a virulent strain of transmissible gastroenteritis virus: comparison of reactivity with virulent and attenuated virus. Arch Virol. 1988;101(3-4):221–235. doi: 10.1007/BF01311003. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES