Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Oct;29(10):2099–2106. doi: 10.1128/jcm.29.10.2099-2106.1991

Heat shock protein 88 and Aspergillus infection.

J P Burnie 1, R C Matthews 1
PMCID: PMC270280  PMID: 1939562

Abstract

Immunoblotting was used to dissect the antibody responses in the sera of 50 patients with proven invasive aspergillosis, 28 patients with suspected invasive aspergillosis, 35 patients with allergic bronchopulmonary aspergillosis, and 10 patients with an aspergilloma. This demonstrated the immunodominance of antigenic bands at 88, 84, 51, and 40 kDa. Monoclonal antibodies against the heat shock protein 90 complexes of Candida albicans and the water mold Achlya ambisexualis identified these four antigenic bands as homologous proteins. Similar antigens have been described in humans, mice, Saccharomyces cerevisiae, chickens, and Drosophila species. The antibody against A. ambisexualis has previously been shown to cross-react with antigens belonging to the human heat shock protein 90 complex. Aspergillus heat shock protein 90 was extracted from the sera of patients with invasive aspergillosis by affinity chromatography. This was done with both a rabbit hyperimmune antiserum raised against an extract of Aspergillus fumigatus NCPF 2109 and a monoclonal antibody against the heat shock protein 90 of C. albicans. In vivo expression of the antigen was demonstrated in an aspergilloma surgically removed from a patient. The role of the antigen as an allergen in allergic bronchopulmonary aspergillosis is also discussed.

Full text

PDF
2099

Images in this article

2101Image
on p.2101
2102Image
on p.2102
2103Image
on p.2103
2103Image
on p.2103

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baur X., Weiss W., Jarosch B., Menz G., Schoch C., Schmitz-Schumann M., Virchow C. Immunoprint pattern in patients with allergic bronchopulmonary aspergillosis in different stages. J Allergy Clin Immunol. 1989 Apr;83(4):839–844. doi: 10.1016/0091-6749(89)90023-7. [DOI] [PubMed] [Google Scholar]
  2. Brouwer J. Detection of antibodies against Aspergillus fumigatus: comparison between double immunodiffusion, ELISA and immunoblot analysis. Int Arch Allergy Appl Immunol. 1988;85(2):244–249. doi: 10.1159/000234510. [DOI] [PubMed] [Google Scholar]
  3. Brummund W., Resnick A., Fink J. N., Kurup V. P. Aspergillus fumigatus-specific antibodies in allergic bronchopulmonary aspergillosis and aspergilloma: evidence for a polyclonal antibody response. J Clin Microbiol. 1987 Jan;25(1):5–9. doi: 10.1128/jcm.25.1.5-9.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brunt S. A., Riehl R., Silver J. C. Steroid hormone regulation of the Achlya ambisexualis 85-kilodalton heat shock protein, a component of the Achlya steroid receptor complex. Mol Cell Biol. 1990 Jan;10(1):273–281. doi: 10.1128/mcb.10.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farrelly F. W., Finkelstein D. B. Complete sequence of the heat shock-inducible HSP90 gene of Saccharomyces cerevisiae. J Biol Chem. 1984 May 10;259(9):5745–5751. [PubMed] [Google Scholar]
  6. Fratamico P. M., Buckley H. R. Identification and characterization of an immunodominant 58-kilodalton antigen of Aspergillus fumigatus recognized by sera of patients with invasive aspergillosis. Infect Immun. 1991 Jan;59(1):309–315. doi: 10.1128/iai.59.1.309-315.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harvey C., Longbottom J. L. Characterization of a major antigenic component of Aspergillus fumigatus. Clin Exp Immunol. 1986 Jul;65(1):206–214. [PMC free article] [PubMed] [Google Scholar]
  8. Haynes K. A., Latge J. P., Rogers T. R. Detection of Aspergillus antigens associated with invasive infection. J Clin Microbiol. 1990 Sep;28(9):2040–2044. doi: 10.1128/jcm.28.9.2040-2044.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hickey E., Brandon S. E., Smale G., Lloyd D., Weber L. A. Sequence and regulation of a gene encoding a human 89-kilodalton heat shock protein. Mol Cell Biol. 1989 Jun;9(6):2615–2626. doi: 10.1128/mcb.9.6.2615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kurup V. P., Greenberger P. A., Fink J. N. Antibody response to low-molecular-weight antigens of Aspergillus fumigatus in allergic bronchopulmonary aspergillosis. J Clin Microbiol. 1989 Jun;27(6):1312–1316. doi: 10.1128/jcm.27.6.1312-1316.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kurup V. P., John K. V., Resnick A., Fink J. N. A partially purified glycoprotein antigen from Aspergillus fumigatus. Int Arch Allergy Appl Immunol. 1986;79(3):263–269. doi: 10.1159/000233984. [DOI] [PubMed] [Google Scholar]
  12. Kurup V. P., Ramasamy M., Greenberger P. A., Fink J. N. Isolation and characterization of a relevant Aspergillus fumigatus antigen with IgG- and IgE-binding activity. Int Arch Allergy Appl Immunol. 1988;86(2):176–182. doi: 10.1159/000234568. [DOI] [PubMed] [Google Scholar]
  13. Leung P. S., Gershwin M. E., Coppel R., Halpern G., Novey H., Castles J. J. Localization, molecular weight and immunoglobulin subclass response to Aspergillus fumigatus allergens in acute bronchopulmonary aspergillosis. Int Arch Allergy Appl Immunol. 1988;85(4):416–421. doi: 10.1159/000234544. [DOI] [PubMed] [Google Scholar]
  14. Lindquist S. The heat-shock response. Annu Rev Biochem. 1986;55:1151–1191. doi: 10.1146/annurev.bi.55.070186.005443. [DOI] [PubMed] [Google Scholar]
  15. Longbottom J. L. Antigens and allergens of Aspergillus fumigatus. II. Their further identification and partial characterization of a major allergen (Ag 3). J Allergy Clin Immunol. 1986 Jul;78(1 Pt 1):18–24. doi: 10.1016/0091-6749(86)90109-0. [DOI] [PubMed] [Google Scholar]
  16. Mason A. B., Brandt M. E., Buckley H. R. Enolase activity associated with a C. albicans cytoplasmic antigen. Yeast. 1989 Apr;5(Spec No):S231–S239. [PubMed] [Google Scholar]
  17. Matthews R. C., Burnie J. P., Tabaqchali S. Isolation of immunodominant antigens from sera of patients with systemic candidiasis and characterization of serological response to Candida albicans. J Clin Microbiol. 1987 Feb;25(2):230–237. doi: 10.1128/jcm.25.2.230-237.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Matthews R., Burnie J. P., Fox A., Tabaqchali S. Immunoblot analysis of serological responses in invasive aspergillosis. J Clin Pathol. 1985 Nov;38(11):1300–1303. doi: 10.1136/jcp.38.11.1300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Matthews R., Burnie J. Cloning of a DNA sequence encoding a major fragment of the 47 kilodalton stress protein homologue of Candida albicans. FEMS Microbiol Lett. 1989 Jul 1;51(1):25–30. doi: 10.1016/0378-1097(89)90071-2. [DOI] [PubMed] [Google Scholar]
  20. Matthews R., Burnie J. Diagnosis of systemic candidiasis by an enzyme-linked dot immunobinding assay for a circulating immunodominant 47-kilodalton antigen. J Clin Microbiol. 1988 Mar;26(3):459–463. doi: 10.1128/jcm.26.3.459-463.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Minota S., Koyasu S., Yahara I., Winfield J. Autoantibodies to the heat-shock protein hsp90 in systemic lupus erythematosus. J Clin Invest. 1988 Jan;81(1):106–109. doi: 10.1172/JCI113280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Moore S. K., Kozak C., Robinson E. A., Ullrich S. J., Appella E. Cloning and nucleotide sequence of the murine hsp84 cDNA and chromosome assignment of related sequences. Gene. 1987;56(1):29–40. doi: 10.1016/0378-1119(87)90155-7. [DOI] [PubMed] [Google Scholar]
  23. Phillips P., Radigan G. Antigenemia in a rabbit model of invasive aspergillosis. J Infect Dis. 1989 Jun;159(6):1147–1150. doi: 10.1093/infdis/159.6.1147. [DOI] [PubMed] [Google Scholar]
  24. Piechura J. E., Huang C. J., Cohen S. H., Kidd J. M., Kurup V. P., Calvanico N. J. Antigens of Aspergillus fumigatus. II. Electrophoretic and clinical studies. Immunology. 1983 Aug;49(4):657–665. [PMC free article] [PubMed] [Google Scholar]
  25. Riehl R. M., Sullivan W. P., Vroman B. T., Bauer V. J., Pearson G. R., Toft D. O. Immunological evidence that the nonhormone binding component of avian steroid receptors exists in a wide range of tissues and species. Biochemistry. 1985 Nov 5;24(23):6586–6591. doi: 10.1021/bi00344a042. [DOI] [PubMed] [Google Scholar]
  26. Schønheyder H. Pathogenetic and serological aspects of pulmonary aspergillosis. Scand J Infect Dis Suppl. 1987;51:1–62. doi: 10.3109/inf.1987.19.suppl-51.01. [DOI] [PubMed] [Google Scholar]
  27. Strockbine N. A., Largen M. T., Zweibel S. M., Buckley H. R. Identification and molecular weight characterization of antigens from Candida albicans that are recognized by human sera. Infect Immun. 1984 Feb;43(2):715–721. doi: 10.1128/iai.43.2.715-721.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tee R. D., Gordon D. J., Taylor A. J. Cross-reactivity between antigens of fungal extracts studied by RAST inhibition and immunoblot technique. J Allergy Clin Immunol. 1987 Apr;79(4):627–633. doi: 10.1016/s0091-6749(87)80159-8. [DOI] [PubMed] [Google Scholar]
  29. Wallenbeck I., Aukrust L., Einarsson R. Antigenic variability of different strains of Aspergillus fumigatus. Int Arch Allergy Appl Immunol. 1984;73(2):166–172. doi: 10.1159/000233459. [DOI] [PubMed] [Google Scholar]
  30. Winfield J. B. Stress proteins, arthritis, and autoimmunity. Arthritis Rheum. 1989 Dec;32(12):1497–1504. doi: 10.1002/anr.1780321202. [DOI] [PubMed] [Google Scholar]
  31. Young R. C., Bennett J. E. Invasive aspergillosis. Absence of detectable antibody response. Am Rev Respir Dis. 1971 Nov;104(5):710–716. doi: 10.1164/arrd.1971.104.5.710. [DOI] [PubMed] [Google Scholar]
  32. Yu B., Niki Y., Armstrong D. Use of immunoblotting to detect Aspergillus fumigatus antigen in sera and urines of rats with experimental invasive aspergillosis. J Clin Microbiol. 1990 Jul;28(7):1575–1579. doi: 10.1128/jcm.28.7.1575-1579.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES