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. 1991 Oct;29(10):2120–2124. doi: 10.1128/jcm.29.10.2120-2124.1991

Identification of Toxoplasma gondii infections by BI gene amplification.

E van de Ven 1, W Melchers 1, J Galama 1, W Camps 1, J Meuwissen 1
PMCID: PMC270284  PMID: 1939564

Abstract

The diagnosis of toxoplasmosis in congenitally infected children or in immunocompromised patients can be difficult; serology is not reliable, and the diagnosis must be based on the combination of symptomatology and the direct demonstration of the parasite in clinical specimens by microscopy, antigen detection, or inoculation of samples into mice or tissue cultures. These techniques are either insensitive or time-consuming. To determine the value of the polymerase chain reaction (PCR) for the diagnosis of Toxoplasma gondii infections, we compared this technique with conventional detection techniques, such as microscopy, tissue culturing, and mouse inoculation. We were able to detect T. gondii by PCR in clinical specimens and tissue samples that were obtained postmortem from a bone marrow recipient with cerebral toxoplasmosis and from three congenitally infected children. The presence of T. gondii was demonstrated in brain tissue, cerebrospinal fluid, the heart, and skeletal muscle tested fresh or after fixation in Formalin. In only one sample was T. gondii isolated by mouse inoculation but not detected by PCR. Because it is a sensitive, relatively rapid, and specific method and because it can be applied to a variety of different clinical samples, PCR can be considered a valuable additional tool for the identification of T. gondii infections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495–503. doi: 10.1128/jcm.28.3.495-503.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brézin A. P., Eqwuagu C. E., Silveira C., Thulliez P., Martins M. C., Mahdi R. M., Belfort R., Jr, Nussenblatt R. B. Analysis of aqueous humor in ocular toxoplasmosis. N Engl J Med. 1991 Mar 7;324(10):699–699. [PubMed] [Google Scholar]
  3. Burg J. L., Grover C. M., Pouletty P., Boothroyd J. C. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol. 1989 Aug;27(8):1787–1792. doi: 10.1128/jcm.27.8.1787-1792.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burg J. L., Perelman D., Kasper L. H., Ware P. L., Boothroyd J. C. Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J Immunol. 1988 Nov 15;141(10):3584–3591. [PubMed] [Google Scholar]
  5. Derouin F., Gluckman E., Beauvais B., Devergie A., Melo R., Monny M., Lariviere M. Toxoplasma infection after human allogeneic bone marrow transplantation: clinical and serological study of 80 patients. Bone Marrow Transplant. 1986 May;1(1):67–73. [PubMed] [Google Scholar]
  6. Desmonts G., Couvreur J. Congenital toxoplasmosis. A prospective study of 378 pregnancies. N Engl J Med. 1974 May 16;290(20):1110–1116. doi: 10.1056/NEJM197405162902003. [DOI] [PubMed] [Google Scholar]
  7. Desmonts G., Daffos F., Forestier F., Capella-Pavlovsky M., Thulliez P., Chartier M. Prenatal diagnosis of congenital toxoplasmosis. Lancet. 1985 Mar 2;1(8427):500–504. doi: 10.1016/s0140-6736(85)92096-3. [DOI] [PubMed] [Google Scholar]
  8. Goelz S. E., Hamilton S. R., Vogelstein B. Purification of DNA from formaldehyde fixed and paraffin embedded human tissue. Biochem Biophys Res Commun. 1985 Jul 16;130(1):118–126. doi: 10.1016/0006-291x(85)90390-0. [DOI] [PubMed] [Google Scholar]
  9. Grover C. M., Thulliez P., Remington J. S., Boothroyd J. C. Rapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid. J Clin Microbiol. 1990 Oct;28(10):2297–2301. doi: 10.1128/jcm.28.10.2297-2301.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heurkens A. H., Koelma I. A., de Planque M. M., Polderman A. M., van der Meer J. W. Failure to diagnose fatal disseminated toxoplasmosis in a bone marrow transplant recipient: the possible significance of declining antibody titres. J Infect. 1989 May;18(3):283–288. doi: 10.1016/s0163-4453(89)80065-9. [DOI] [PubMed] [Google Scholar]
  11. Holliman R. E., Johnson J. D., Savva D. Diagnosis of cerebral toxoplasmosis in association with AIDS using the polymerase chain reaction. Scand J Infect Dis. 1990;22(2):243–244. doi: 10.3109/00365549009037911. [DOI] [PubMed] [Google Scholar]
  12. Koppe J. G., Loewer-Sieger D. H., de Roever-Bonnet H. Results of 20-year follow-up of congenital toxoplasmosis. Lancet. 1986 Feb 1;1(8475):254–256. doi: 10.1016/s0140-6736(86)90785-3. [DOI] [PubMed] [Google Scholar]
  13. Kwok S., Higuchi R. Avoiding false positives with PCR. Nature. 1989 May 18;339(6221):237–238. doi: 10.1038/339237a0. [DOI] [PubMed] [Google Scholar]
  14. Luft B. J., Hafner R. Toxoplasmic encephalitis. AIDS. 1990 Jun;4(6):593–595. doi: 10.1097/00002030-199006000-00018. [DOI] [PubMed] [Google Scholar]
  15. Luft B. J., Remington J. S. AIDS commentary. Toxoplasmic encephalitis. J Infect Dis. 1988 Jan;157(1):1–6. doi: 10.1093/infdis/157.1.1. [DOI] [PubMed] [Google Scholar]
  16. Shepp D. H., Hackman R. C., Conley F. K., Anderson J. B., Meyers J. D. Toxoplasma gondii reactivation identified by detection of parasitemia in tissue culture. Ann Intern Med. 1985 Aug;103(2):218–221. doi: 10.7326/0003-4819-103-2-218. [DOI] [PubMed] [Google Scholar]
  17. Tokuda Y., Nakamura T., Satonaka K., Maeda S., Doi K., Baba S., Sugiyama T. Fundamental study on the mechanism of DNA degradation in tissues fixed in formaldehyde. J Clin Pathol. 1990 Sep;43(9):748–751. doi: 10.1136/jcp.43.9.748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. van Loon A. M., van der Logt J. T., Heessen F. W., van der Veen J. Enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Jun;17(6):997–1004. doi: 10.1128/jcm.17.6.997-1004.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Loon A. M., van der Veen J. Enzyme-linked immunosorbent assay for quantitation of toxoplasma antibodies in human sera. J Clin Pathol. 1980 Jul;33(7):635–639. doi: 10.1136/jcp.33.7.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van Nunen M. C., van der Veen J. Examination for toxoplasmosis by the fluorescent antibody technique. Trop Geogr Med. 1965 Sep;17(3):246–253. [PubMed] [Google Scholar]

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