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. 1991 Oct;29(10):2224–2227. doi: 10.1128/jcm.29.10.2224-2227.1991

Cultivation and characterization of novel human group A rotaviruses with long RNA electropherotypes, subgroup II specificities, and serotype 2 VP7 genes.

F Bingnan 1, L E Unicomb 1, G L Tu 1, A Ali 1, A Malek 1, Z Rahim 1, S Tzipori 1
PMCID: PMC270302  PMID: 1658036

Abstract

During an epidemiological study of human rotavirus infections in Bangladesh, three group A strains hybridized with a serotype 2 oligonucleotide probe, but they had long RNA electropherotypes. The three strains were collected from 8- to 20-month-old infants with acute diarrhea and moderate malnutrition. By a modified isolation procedure, two strains (T-B and T-C) were adapted in MA104 cell cultures. They were identified to be subgroup II specific by enzyme-linked immunosorbent assay with subgroup I- and II-specific monoclonal antibodies and were identified by a fluorescent focus reduction neutralization assay with hyperimmune antisera to be serotype 2 specific. Further characterization of these unusual rotavirus strains needs to be carried out.

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Selected References

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