Abstract
A simple, efficient method for identification and differentiation of Yersinia enterocolitica containing virulent plasmid-bearing clones is described. The method is based on temperature of incubation and the low calcium response of the organism in an agarose Congo red medium.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bhaduri S. Calcium-responsive expression of plasmid-mediated outer membrane proteins from Yersinia enterocolitica grown on solid media. J Ind Microbiol. 1990 Jun;5(4):207–214. doi: 10.1007/BF01569678. [DOI] [PubMed] [Google Scholar]
- Bhaduri S., Conway L. K., Lachica R. V. Assay of crystal violet binding for rapid identification of virulent plasmid-bearing clones of Yersinia enterocolitica. J Clin Microbiol. 1987 Jun;25(6):1039–1042. doi: 10.1128/jcm.25.6.1039-1042.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bhaduri S. Evaluation of different techniques for detection of virulence in Yersinia enterocolitica. J Clin Microbiol. 1990 Apr;28(4):828–829. doi: 10.1128/jcm.28.4.828-829.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bhaduri S., Turner-Jones C., Taylor M. M., Lachica R. V. Simple assay of calcium dependency for virulent plasmid-bearing clones of Yersinia enterocolitica. J Clin Microbiol. 1990 Apr;28(4):798–800. doi: 10.1128/jcm.28.4.798-800.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cornelis G., Laroche Y., Balligand G., Sory M. P., Wauters G. Yersinia enterocolitica, a primary model for bacterial invasiveness. Rev Infect Dis. 1987 Jan-Feb;9(1):64–87. doi: 10.1093/clinids/9.1.64. [DOI] [PubMed] [Google Scholar]
- Goguen J. D., Yother J., Straley S. C. Genetic analysis of the low calcium response in Yersinia pestis mu d1(Ap lac) insertion mutants. J Bacteriol. 1984 Dec;160(3):842–848. doi: 10.1128/jb.160.3.842-848.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lachica R. V., Zink D. L. Determination of plasmid-associated hydrophobicity of Yersinia enterocolitica by a latex particle agglutination test. J Clin Microbiol. 1984 May;19(5):660–663. doi: 10.1128/jcm.19.5.660-663.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miliotis M. D., Galen J. E., Kaper J. B., Morris J. G., Jr Development and testing of a synthetic oligonucleotide probe for the detection of pathogenic Yersinia strains. J Clin Microbiol. 1989 Jul;27(7):1667–1670. doi: 10.1128/jcm.27.7.1667-1670.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Portnoy D. A., Martinez R. J. Role of a plasmid in the pathogenicity of Yersinia species. Curr Top Microbiol Immunol. 1985;118:29–51. doi: 10.1007/978-3-642-70586-1_3. [DOI] [PubMed] [Google Scholar]
- Prpic J. K., Robins-Browne R. M., Davey R. B. Differentiation between virulent and avirulent Yersinia enterocolitica isolates by using Congo red agar. J Clin Microbiol. 1983 Sep;18(3):486–490. doi: 10.1128/jcm.18.3.486-490.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prpic J. K., Robins-Browne R. M., Davey R. B. In vitro assessment of virulence in Yersinia enterocolitica and related species. J Clin Microbiol. 1985 Jul;22(1):105–110. doi: 10.1128/jcm.22.1.105-110.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riley G., Toma S. Detection of pathogenic Yersinia enterocolitica by using congo red-magnesium oxalate agar medium. J Clin Microbiol. 1989 Jan;27(1):213–214. doi: 10.1128/jcm.27.1.213-214.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]