Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Nov;29(11):2434–2437. doi: 10.1128/jcm.29.11.2434-2437.1991

Variability in growth of Neisseria polysaccharea on colistin-containing selective media for Neisseria spp.

C M Anand 1, F Ashton 1, H Shaw 1, R Gordon 1
PMCID: PMC270351  PMID: 1774246

Abstract

In a prospective survey of 773 healthy schoolchildren in southern Alberta, Canada, Neisseria polysaccharea was isolated from the pharynxes of only 4 (0.5%) subjects, whereas Neisseria lactamica and Neisseria meningitidis were isolated from 110 (14%) and 15 (2%) children, respectively. These strains of N. polysaccharea, together with three other sporadic isolates from Alberta, Canada, were compared with the type strain from France and strains from Spain and Germany. All strains were phenotypically identical, except that the Canadian and German strains, for which the colistin MICs were 1 mg/liter, failed to grow on Thayer-Martin medium (TMM), whereas the type strain and the Spanish strains, for which the colistin MICs were greater than 7.5 mg/liter, were not inhibited. Multilocus enzyme electrophoresis indicated that six distinct electrophoretic types were present among the seven Canadian strains. Our results show that growth on gonococcal selective media which contain colistin is a variable feature of this taxon.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger U. First isolation of Neisseria polysacchareae species nova in the Federal Republic of Germany. Eur J Clin Microbiol. 1985 Aug;4(4):431–433. doi: 10.1007/BF02148705. [DOI] [PubMed] [Google Scholar]
  2. Boquete M. T., Marcos C., Sáez-Nieto J. A. Characterization of Neisseria polysacchareae sp. nov. (Riou, 1983) in previously identified noncapsular strains of Neisseria meningitidis. J Clin Microbiol. 1986 May;23(5):973–975. doi: 10.1128/jcm.23.5.973-975.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cann K. J., Rogers T. R., Jones D. M., Noah N. D., Burns C. Neisseria meningitidis in a primary school. Arch Dis Child. 1987 Nov;62(11):1113–1117. doi: 10.1136/adc.62.11.1113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cann K. J., Rogers T. R. The phenotypic relationship of Neisseria polysaccharea to commensal and pathogenic Neisseria spp. J Med Microbiol. 1989 Aug;29(4):251–254. doi: 10.1099/00222615-29-4-251. [DOI] [PubMed] [Google Scholar]
  5. Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
  6. Flynn J., Waitkins S. A. A serum-free medium for testing fermentation reactions in Neisseria gonorrhoeae. J Clin Pathol. 1972 Jun;25(6):525–527. doi: 10.1136/jcp.25.6.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gold R., Goldschneider I., Lepow M. L., Draper T. F., Randolph M. Carriage of Neisseria meningitidis and Neisseria lactamica in infants and children. J Infect Dis. 1978 Feb;137(2):112–121. doi: 10.1093/infdis/137.2.112. [DOI] [PubMed] [Google Scholar]
  8. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Knapp J. S., Holmes K. K. Modified oxidation-fermentation medium for detection of acid production from carbohydrates by Neisseria spp. and Branhamella catarrhalis. J Clin Microbiol. 1983 Jul;18(1):56–62. doi: 10.1128/jcm.18.1.56-62.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Riou J. Y., Guibourdenche M., Perry M. B., MacLean L. L., Griffith D. W. Structure of the exocellular D-glucan produced by Neisseria polysaccharea. Can J Microbiol. 1986 Dec;32(12):909–911. doi: 10.1139/m86-167. [DOI] [PubMed] [Google Scholar]
  11. Riou J. Y., Guibourdenche M., Popoff M. Y. A new taxon in the genus Neisseria. Ann Microbiol (Paris) 1983 Sep-Oct;134B(2):257–267. doi: 10.1016/s0769-2609(83)80038-6. [DOI] [PubMed] [Google Scholar]
  12. Saez-Nieto J. A., Dominguez J. R., Monton J. L., Cristobal P., Fenoll A., Vazquez J., Casal J., Taracena B. Carriage of Neisseria meningitidis and Neisseria lactamica in a school population during an epidemic period in Spain. J Hyg (Lond) 1985 Jun;94(3):279–288. doi: 10.1017/s0022172400061507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Saez-Nieto J. A., Lujan R., Martinez-Suarez J. V., Berron S., Vazquez J. A., Viñas M., Campos J. Neisseria lactamica and Neisseria polysaccharea as possible sources of meningococcal beta-lactam resistance by genetic transformation. Antimicrob Agents Chemother. 1990 Nov;34(11):2269–2272. doi: 10.1128/aac.34.11.2269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shtibel R., Toma S. Neisseria gonorrhoeae: evaluation of some methods used for carbohydrate utilization. Can J Microbiol. 1978 Feb;24(2):177–181. doi: 10.1139/m78-030. [DOI] [PubMed] [Google Scholar]
  16. Thayer J. D., Martin J. E., Jr Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis. Public Health Rep. 1966 Jun;81(6):559–562. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES