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. 2009 Mar 19;150(7):3425–3432. doi: 10.1210/en.2008-1738

Figure 4.

Figure 4

Acetylation and methylation status in the presence of the F455S mutant TR. ChIP assays were performed with cells expressing the WT TR (white bars) or F455S mutant TR (black bars) using antibodies against acetylated H3K9 (AcH3K9) (A), acetylated H3K14 (AcH3K14) (B), diMeH3K4 (C), and triMeH3K4 (D). The values are expressed relative to the WT cells (white bars), and the value for the WT cells at 0 min was set as 1.0. A and B, Residues H3K9 and -K14 were hyperacetylated, and reacetylation started earlier in F455S cells. C and D, No significant changes in diMeH3K4 or triMeH3K4 were observed at the basal level of the TRH gene compared with that of the WT TR. After the addition of 100 nm T3, the profile of diMeH3K4 and triMeH3K4 in F455S cells was similar to that in WT cells. The data are presented as the mean ± se for three independent experiments. Asterisks indicate a significant difference vs. 0 min: *, P < 0.05; **, P < 0.01. n.s., Not significant.