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. 2009 Apr 2;150(7):3345–3352. doi: 10.1210/en.2008-1449

Figure 3.

Figure 3

CCL7 induces cumulus cell rounding by a RhoA-dependent pathway. A, Effect of CCL7 on the size distribution of cumulus cells (left panel). COCs were incubated with CCL7 in the presence or absence of vMIP-II for 3 h. Cumulus cells were then subjected to FACS analysis. The percentages of cells within each FSC-height region are also shown as a bar graph (right panel). *, P < 0.01. B, Effects of Rho pathway inhibitors on CCL7-induced cell contraction. COCs were incubated with CCL7 in the presence of vehicle (Cont), Y27632, or blebbistatin. Cumulus cells were subjected to FACS analysis and the results are shown as a bar graph. *, P < 0.01. C, CCL7 induces RhoA activation in COCs. COCs were incubated with CCL7 in the presence or absence of vMIP-II for 10 min and then subjected to the RhoA pull-down assay. Representative results of three (A and B) or two (C) independent experiments are shown.