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. 1991 Nov;29(11):2513–2516. doi: 10.1128/jcm.29.11.2513-2516.1991

Epidemiology of an endemic strain of beta-lactamase-producing Enterococcus faecalis.

J E Patterson 1, K V Singh 1, B E Murray 1
PMCID: PMC270364  PMID: 1774257

Abstract

Three previously reported beta-lactamase-producing (Bla+) enterococci with distinct but related antibiotic resistance phenotypes, plasmid profiles, and plasmid restriction endonuclease digestion patterns were isolated at the West Haven Veterans Administration Medical Center in Connecticut (WH245, WH257, WH571) in July 1986 and March 1987. In this study, we analyzed the whole-cell DNA of these isolates by using pulsed-field gel electrophoresis of large chromosomal fragments generated by SmaI digestion. The three West Haven isolates showed very similar chromosomal restriction endonuclease digestion patterns; these patterns were distinct from those of Bla+ and Bla- enterococci from other geographic areas and from those of other West Haven enterococci, suggesting an endemic Bla+ strain at this institution. Clinical information regarding these isolates suggests that exposure to the genitourinary clinic, obstructive urinary tract disease, and frequent antibiotic therapy may have been common risk factors for acquisition of this endemic strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Murray B. E., Church D. A., Wanger A., Zscheck K., Levison M. E., Ingerman M. J., Abrutyn E., Mederski-Samoraj B. Comparison of two beta-lactamase-producing strains of Streptococcus faecalis. Antimicrob Agents Chemother. 1986 Dec;30(6):861–864. doi: 10.1128/aac.30.6.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Murray B. E., Mederski-Samaroj B. Transferable beta-lactamase. A new mechanism for in vitro penicillin resistance in Streptococcus faecalis. J Clin Invest. 1983 Sep;72(3):1168–1171. doi: 10.1172/JCI111042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Murray B. E., Singh K. V., Heath J. D., Sharma B. R., Weinstock G. M. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J Clin Microbiol. 1990 Sep;28(9):2059–2063. doi: 10.1128/jcm.28.9.2059-2063.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Murray B. E., Singh K. V., Markowitz S. M., Lopardo H. A., Patterson J. E., Zervos M. J., Rubeglio E., Eliopoulos G. M., Rice L. B., Goldstein F. W. Evidence for clonal spread of a single strain of beta-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states. J Infect Dis. 1991 Apr;163(4):780–785. doi: 10.1093/infdis/163.4.780. [DOI] [PubMed] [Google Scholar]
  5. Nicolle L. E., Bjornson J., Harding G. K., MacDonell J. A. Bacteriuria in elderly institutionalized men. N Engl J Med. 1983 Dec 8;309(23):1420–1425. doi: 10.1056/NEJM198312083092304. [DOI] [PubMed] [Google Scholar]
  6. Patterson J. E., Masecar B. L., Kauffman C. A., Schaberg D. R., Hierholzer W. J., Jr, Zervos M. J. Gentamicin resistance plasmids of enterococci from diverse geographic areas are heterogeneous. J Infect Dis. 1988 Jul;158(1):212–216. doi: 10.1093/infdis/158.1.212. [DOI] [PubMed] [Google Scholar]
  7. Patterson J. E., Masecar B. L., Zervos M. J. Characterization and comparison of two penicillinase-producing strains of Streptococcus (Enterococcus) faecalis. Antimicrob Agents Chemother. 1988 Jan;32(1):122–124. doi: 10.1128/aac.32.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Patterson J. E., Wanger A., Zscheck K. K., Zervos M. J., Murray B. E. Molecular epidemiology of beta-lactamase-producing enterococci. Antimicrob Agents Chemother. 1990 Feb;34(2):302–305. doi: 10.1128/aac.34.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Patterson J. E., Zervos M. J. Susceptibility and bactericidal activity studies of four beta-lactamase-producing enterococci. Antimicrob Agents Chemother. 1989 Feb;33(2):251–253. doi: 10.1128/aac.33.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rhinehart E., Smith N. E., Wennersten C., Gorss E., Freeman J., Eliopoulos G. M., Moellering R. C., Jr, Goldmann D. A. Rapid dissemination of beta-lactamase-producing, aminoglycoside-resistant Enterococcus faecalis among patients and staff on an infant-toddler surgical ward. N Engl J Med. 1990 Dec 27;323(26):1814–1818. doi: 10.1056/NEJM199012273232606. [DOI] [PubMed] [Google Scholar]
  11. Zervos M. J., Dembinski S., Mikesell T., Schaberg D. R. High-level resistance to gentamicin in Streptococcus faecalis: risk factors and evidence for exogenous acquisition of infection. J Infect Dis. 1986 Jun;153(6):1075–1083. doi: 10.1093/infdis/153.6.1075. [DOI] [PubMed] [Google Scholar]

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