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. 1991 Dec;29(12):2731–2734. doi: 10.1128/jcm.29.12.2731-2734.1991

Differentiation of Lactococcus lactis and Lactococcus garvieae from humans by comparison of whole-cell protein patterns.

J A Elliott 1, M D Collins 1, N E Pigott 1, R R Facklam 1
PMCID: PMC270423  PMID: 1757541

Abstract

We tested 12 reference and 24 clinical strains of lactococci for physiologic characteristics using a conventional test system, the Gen-Probe Enterococcus 2 chemiluminescence assay (Gen-Probe Inc., San Diego, Calif.), the Rapid Strep identification system (Analytab Products, Plainview, N.Y.), and whole-cell protein analysis. The Gen-Probe Enterococcus 2 chemiluminescence assay for Enterococcus identification was negative with all strains. Neither the conventional test nor the Rapid Strep identification system could differentiate between the two Lactococcus spp. most commonly isolated from humans. A simple procedure, based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was developed for comparing the whole-cell protein patterns of Lactococcus spp. L. lactis and L. garvieae were differentiated by unique protein patterns.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Collins M. D., Ash C., Farrow J. A., Wallbanks S., Williams A. M. 16S ribosomal ribonucleic acid sequence analyses of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov. J Appl Bacteriol. 1989 Oct;67(4):453–460. doi: 10.1111/j.1365-2672.1989.tb02516.x. [DOI] [PubMed] [Google Scholar]
  2. Collins M. D., Farrow J. A., Phillips B. A., Kandler O. Streptococcus garvieae sp. nov. and Streptococcus plantarum sp. nov. J Gen Microbiol. 1983 Nov;129(11):3427–3431. doi: 10.1099/00221287-129-11-3427. [DOI] [PubMed] [Google Scholar]
  3. Collins M. D., Rodrigues U. M., Pigott N. E., Facklam R. R. Enterococcus dispar sp. nov. a new Enterococcus species from human sources. Lett Appl Microbiol. 1991 Mar;12(3):95–98. doi: 10.1111/j.1472-765x.1991.tb00514.x. [DOI] [PubMed] [Google Scholar]
  4. Colman G., Ball L. C. Identification of streptococci in a medical laboratory. J Appl Bacteriol. 1984 Aug;57(1):1–14. doi: 10.1111/j.1365-2672.1984.tb02351.x. [DOI] [PubMed] [Google Scholar]
  5. Elliott J. A., Facklam R. R., Richter C. B. Whole-cell protein patterns of nonhemolytic group B, type Ib, streptococci isolated from humans, mice, cattle, frogs, and fish. J Clin Microbiol. 1990 Mar;28(3):628–630. doi: 10.1128/jcm.28.3.628-630.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Facklam R. R., Collins M. D. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J Clin Microbiol. 1989 Apr;27(4):731–734. doi: 10.1128/jcm.27.4.731-734.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Facklam R., Hollis D., Collins M. D. Identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria. J Clin Microbiol. 1989 Apr;27(4):724–730. doi: 10.1128/jcm.27.4.724-730.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Farrow J. A., Jones D., Phillips B. A., Collins M. D. Taxonomic studies on some group D streptococci. J Gen Microbiol. 1983 May;129(5):1423–1432. doi: 10.1099/00221287-129-5-1423. [DOI] [PubMed] [Google Scholar]
  9. Jarvis A. W., Wolff J. M. Grouping of lactic streptococci by gel electrophoresis of soluble cell extracts. Appl Environ Microbiol. 1979 Mar;37(3):391–398. doi: 10.1128/aem.37.3.391-398.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mannion P. T., Rothburn M. M. Diagnosis of bacterial endocarditis caused by Streptococcus lactis and assisted by immunoblotting of serum antibodies. J Infect. 1990 Nov;21(3):317–318. doi: 10.1016/0163-4453(90)94149-t. [DOI] [PubMed] [Google Scholar]
  11. Parker M. T., Ball L. C. Streptococci and aerococci associated with systemic infection in man. J Med Microbiol. 1976 Aug;9(3):275–302. doi: 10.1099/00222615-9-3-275. [DOI] [PubMed] [Google Scholar]
  12. Päkkilä M., Forsén R. Comparison of membrane proteins from lactic Streptococci by gel electrophoresis. Acta Chem Scand B. 1979;33(9):632–636. doi: 10.3891/acta.chem.scand.33b-0632. [DOI] [PubMed] [Google Scholar]
  13. WOOD H. F., JACOBS K., McCARTY M. Streptococcus lactis isolated from a patient with subacute bacterial endocarditis. Am J Med. 1955 Feb;18(2):345–347. doi: 10.1016/0002-9343(55)90247-3. [DOI] [PubMed] [Google Scholar]

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