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. 1991 Dec;29(12):2831–2834. doi: 10.1128/jcm.29.12.2831-2834.1991

Detection of Helicobacter pylori in stomach tissue by use of a monoclonal antibody.

M O Husson 1, H Leclerc 1
PMCID: PMC270441  PMID: 1721923

Abstract

Monoclonal antibodies were produced against an acid glycine extract of Helicobacter pylori ATCC 43504T. One of these appeared to be specific for H. pylori; it recognized all H. pylori isolates by an indirect immunofluorescence assay (IIF) but it did not cross-react with the other strains tested, including different species of the genera Helicobacter, Campylobacter, and Wolinella. Different strains of members of the families Enterobacteriaceae and Pseudomonadaceae or other gram-negative bacteria tested also gave negative reactions. Indirect immunofluorescence assay of antral biopsy specimens identified 54 of 56 infected patients (96.4%), and it may be able to detect nonviable organisms after antibiotic therapy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaser M. J., Duncan D. J. Human serum antibody response to Campylobacter jejuni infection as measured in an enzyme-linked immunosorbent assay. Infect Immun. 1984 May;44(2):292–298. doi: 10.1128/iai.44.2.292-298.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buck G. E., Gourley W. K., Lee W. K., Subramanyam K., Latimer J. M., DiNuzzo A. R. Relation of Campylobacter pyloridis to gastritis and peptic ulcer. J Infect Dis. 1986 Apr;153(4):664–669. doi: 10.1093/infdis/153.4.664. [DOI] [PubMed] [Google Scholar]
  3. Dunn B. E., Perez-Perez G. I., Blaser M. J. Two-dimensional gel electrophoresis and immunoblotting of Campylobacter pylori proteins. Infect Immun. 1989 Jun;57(6):1825–1833. doi: 10.1128/iai.57.6.1825-1833.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engstrand L., Pählson C., Gustavsson S., Schwan A. Monoclonal antibodies for rapid identification of Campylobacter pyloridis. Lancet. 1986 Dec 13;2(8520):1402–1403. doi: 10.1016/s0140-6736(86)92049-0. [DOI] [PubMed] [Google Scholar]
  5. Gledhill T., Leicester R. J., Addis B., Lightfoot N., Barnard J., Viney N., Darkin D., Hunt R. H. Epidemic hypochlorhydria. Br Med J (Clin Res Ed) 1985 May 11;290(6479):1383–1386. doi: 10.1136/bmj.290.6479.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goodwin C. S., Blincow E., Peterson G., Sanderson C., Cheng W., Marshall B., Warren J. R., McCulloch R. Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C. pyloridis in the gastric mucosa. J Infect Dis. 1987 Mar;155(3):488–494. doi: 10.1093/infdis/155.3.488. [DOI] [PubMed] [Google Scholar]
  7. Morris A., Ali M. R., Brown P., Lane M., Patton K. Campylobacter pylori infection in biopsy specimens of gastric antrum: laboratory diagnosis and estimation of sampling error. J Clin Pathol. 1989 Jul;42(7):727–732. doi: 10.1136/jcp.42.7.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Negrini R., Lisato L., Cavazzini L., Maini P., Gullini S., Basso O., Lanza G., Jr, Garofalo M., Nenci I. Monoclonal antibodies for specific immunoperoxidase detection of Campylobacter pylori. Gastroenterology. 1989 Feb;96(2 Pt 1):414–420. doi: 10.1016/0016-5085(89)91565-5. [DOI] [PubMed] [Google Scholar]
  9. Newell D. G. Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C. pyloridis and C. jejuni. J Gen Microbiol. 1987 Jan;133(1):163–170. doi: 10.1099/00221287-133-1-163. [DOI] [PubMed] [Google Scholar]
  10. Taylor D. E., Hargreaves J. A., Ng L. K., Sherbaniuk R. W., Jewell L. D. Isolation and characterization of Campylobacter pyloridis from gastric biopsies. Am J Clin Pathol. 1987 Jan;87(1):49–54. doi: 10.1093/ajcp/87.1.49. [DOI] [PubMed] [Google Scholar]
  11. Valentine J. L., Arthur R. R., Mobley H. L., Dick J. D. Detection of Helicobacter pylori by using the polymerase chain reaction. J Clin Microbiol. 1991 Apr;29(4):689–695. doi: 10.1128/jcm.29.4.689-695.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Van den Berg F. M., Zijlmans H., Langenberg W., Rauws E., Schipper M. Detection of Campylobacter pylori in stomach tissue by DNA in situ hybridisation. J Clin Pathol. 1989 Sep;42(9):995–1000. doi: 10.1136/jcp.42.9.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Westblom T. U., Madan E., Kemp J., Subik M. A. Evaluation of a rapid urease test to detect Campylobacter pylori infection. J Clin Microbiol. 1988 Jul;26(7):1393–1394. doi: 10.1128/jcm.26.7.1393-1394.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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