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. 1991 Dec;29(12):2850–2855. doi: 10.1128/jcm.29.12.2850-2855.1991

Use of multiple markers for investigation of an epidemic of Shigella sonnei infections in Monroe County, New York.

P Yagupsky 1, M Loeffelholz 1, K Bell 1, M A Menegus 1
PMCID: PMC270445  PMID: 1757559

Abstract

Antibiotic susceptibility patterns, plasmid profiles, and endonuclease restriction analysis of plasmid DNA were used in the investigation of an epidemic of Shigella sonnei infections in Monroe County, New York, in 1988 and 1989. The epidemic peaked during the winter, included the simultaneous transmission of the disease from person to person and from common food sources, and especially affected inhabitants of the poor, inner-city neighborhoods, young children of both sexes, and women. Resistance to ampicillin, tetracycline, or trimethoprim-sulfamethoxazole, encoded in a 70-MDa plasmid, was found in most of the examined isolates. Unexpectedly, isolates from patients involved in a food-borne outbreak exhibited three different antibiotic susceptibility patterns, suggesting deletion of antibiotic resistance determinants in some strains. Antibiograms clearly separated food-borne outbreak-related and non-foodborne outbreak-related strains, distinguished more strains than did the plasmid profiles, and were useful in tracing the dissemination of individual isolates in the community. Restriction endonuclease analysis substantially increased the discriminatory value of plasmid profiles and validated the antibiogram results. The present study illustrates the complexity of epidemics of S. sonnei infections and shows the value of combining different biological markers in the investigation.

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Selected References

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  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Black R. E., Craun G. F., Blake P. A. Epidemiology of common-source outbreaks of shigellosis in the United States, 1961-1975. Am J Epidemiol. 1978 Jul;108(1):47–52. [PubMed] [Google Scholar]
  4. Blaser M. J., Pollard R. A., Feldman R. A. Shigella infections in the United States, 1974-1980. J Infect Dis. 1983 Apr;147(4):771–775. doi: 10.1093/infdis/147.4.771. [DOI] [PubMed] [Google Scholar]
  5. Bratoeva M. P. Microbiological methods and plasmid pattern analysis in epidemiological control of Shigella sonnei infections. Microbiol Sci. 1986 Jul;3(7):216–219. [PubMed] [Google Scholar]
  6. Christie A. B. Bacillary dysentery. Br Med J. 1968 May 4;2(5600):285–288. doi: 10.1136/bmj.2.5600.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cooksey R. C., Clark N. C., Thornsberry C. A gene probe for TEM type beta-lactamases. Antimicrob Agents Chemother. 1985 Jul;28(1):154–156. doi: 10.1128/aac.28.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Davis H., Taylor J. P., Perdue J. N., Stelma G. N., Jr, Humphreys J. M., Jr, Rowntree R., 3rd, Greene K. D. A shigellosis outbreak traced to commercially distributed shredded lettuce. Am J Epidemiol. 1988 Dec;128(6):1312–1321. doi: 10.1093/oxfordjournals.aje.a115084. [DOI] [PubMed] [Google Scholar]
  9. Donadio J. A., Gangarosa E. J. From the national communicable disease center. J Infect Dis. 1969 Jun;119(6):666–668. doi: 10.1093/infdis/119.6.666. [DOI] [PubMed] [Google Scholar]
  10. Haider K., Huq M. I., Samadi A. R., Ahmad K. Plasmid characterization of Shigella spp. isolated from children with shigellosis and asymptomatic excretors. J Antimicrob Chemother. 1985 Dec;16(6):691–698. doi: 10.1093/jac/16.6.691. [DOI] [PubMed] [Google Scholar]
  11. Keusch G. T. Shigella infections. Clin Gastroenterol. 1979 Sep;8(3):645–662. [PubMed] [Google Scholar]
  12. Kostrzewski J., Stypulkowska-Misiurewicz Changes in the epidemiology of dysentery in Poland and the situation in Europe. Arch Immunol Ther Exp (Warsz) 1968;16(3):429–451. [PubMed] [Google Scholar]
  13. Kuhns D. M., Anderson T. G. A Fly-borne Bacillary Dysentery Epidemic in a Large Military Organization. Am J Public Health Nations Health. 1944 Jul;34(7):750–755. doi: 10.2105/ajph.34.7.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lee L. A., Ostroff S. M., McGee H. B., Johnson D. R., Downes F. P., Cameron D. N., Bean N. H., Griffin P. M. An outbreak of shigellosis at an outdoor music festival. Am J Epidemiol. 1991 Mar 15;133(6):608–615. doi: 10.1093/oxfordjournals.aje.a115933. [DOI] [PubMed] [Google Scholar]
  15. MOSLEY W. H., ADAMS B., LYMAN E. D. Epidemiologic and sociologic features of a large urban outbreak of shigellosis. JAMA. 1962 Dec 29;182:1307–1311. doi: 10.1001/jama.1962.03050520005002. [DOI] [PubMed] [Google Scholar]
  16. Marranzano M., Giammanco G., d'Hauteville H., Sansonetti P. Epidemiological markers of Shigella sonnei infections: R-plasmid fingerprinting, phage-typing and biotyping. Ann Inst Pasteur Microbiol. 1985 May-Jun;136A(3):339–345. doi: 10.1016/s0769-2609(85)80096-x. [DOI] [PubMed] [Google Scholar]
  17. Martin D. L., Gustafson T. L., Pelosi J. W., Suarez L., Pierce G. V. Contaminated produce--a common source for two outbreaks of Shigella gastroenteritis. Am J Epidemiol. 1986 Aug;124(2):299–305. doi: 10.1093/oxfordjournals.aje.a114388. [DOI] [PubMed] [Google Scholar]
  18. Mendoza M. C., Gonzalez A. J., Mendez F. J., Hardisson C. Plasmid typing of Shigella sonnei epidemic strains and molecular relationship of their R-plasmids. Eur J Epidemiol. 1988 Jun;4(2):158–163. doi: 10.1007/BF00144744. [DOI] [PubMed] [Google Scholar]
  19. Prado D., Murray B. E., Cleary T. G., Pickering L. K. Limitations of using the plasmid pattern as an epidemiological tool for clinical isolates of Shigella sonnei. J Infect Dis. 1987 Feb;155(2):314–316. doi: 10.1093/infdis/155.2.314. [DOI] [PubMed] [Google Scholar]
  20. ROSS A. I. Sonne dysentery in Leicester. Mon Bull Minist Health Public Health Lab Serv. 1955 Feb;14:16–32. [PubMed] [Google Scholar]
  21. Reller L. B., Gangarosa E. J., Brachman P. S. From the national communicable disease center. Shigellosis in the United States. 1964-1968. J Infect Dis. 1969 Sep;120(3):393–396. doi: 10.1093/infdis/120.3.393. [DOI] [PubMed] [Google Scholar]
  22. Smith G. E., Summers M. D. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Anal Biochem. 1980 Nov 15;109(1):123–129. doi: 10.1016/0003-2697(80)90019-6. [DOI] [PubMed] [Google Scholar]
  23. Tacket C. O., Cohen M. L. Shigellosis in day care centers: use of plasmid analysis to assess control measures. Pediatr Infect Dis. 1983 Mar-Apr;2(2):127–130. [PubMed] [Google Scholar]
  24. Tacket C. O., Shahid N., Huq M. I., Alim A. R., Cohen M. L. Usefulness of plasmid profiles for differentiation of Shigella isolates in Bangladesh. J Clin Microbiol. 1984 Aug;20(2):300–301. doi: 10.1128/jcm.20.2.300-301.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weissman J. B., Schmerler A., Weiler P., Filice G., Godbey N., Hansen I. The role of preschool children and day-care centers in the spread of shigellosis in urban communities. J Pediatr. 1974 Jun;84(6):797–802. doi: 10.1016/s0022-3476(74)80750-x. [DOI] [PubMed] [Google Scholar]

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