Abstract
Serum (n = 1,636) and egg yolk (n = 802) samples collected from hens on four commercial egg farms in Florida were tested for the presence of specific antibodies to Mycoplasma gallisepticum in a commercially available enzyme-linked immunosorbent assay. No significant differences were noted between serum and egg yolk samples with respect to distribution of positive, suspect, and negative test results or for the mean sample/positive control ratio values of positive, suspect, and negative test results. A linear relationship between the distribution of positive and negative results and the age of the birds was observed for results obtained with both serum and egg yolk samples. On the basis of the results of this study, egg yolk samples can be used in lieu of serum samples to screen flocks for antibodies to Mycoplasma gallisepticum.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cummings T. S., Kleven S. H. Evaluation of protection against Mycoplasma gallisepticum infection in chickens vaccinated with the F strain of M. gallisepticum. Avian Dis. 1986 Jan-Mar;30(1):169–171. [PubMed] [Google Scholar]
- Glisson J. R., Dawe J. F., Kleven S. H. The effect of oil-emulsion vaccines on the occurrence of nonspecific plate agglutination reactions for Mycoplasma gallisepticum and M. synoviae. Avian Dis. 1984 Apr-Jun;28(2):397–405. [PubMed] [Google Scholar]
- Glisson J. R., Kleven S. H. Mycoplasma gallisepticum vaccination: effects on egg transmission and egg production. Avian Dis. 1984 Apr-Jun;28(2):406–415. [PubMed] [Google Scholar]
- Glisson J. R., Kleven S. H. Mycoplasma gallisepticum vaccination: further studies on egg transmission and egg production. Avian Dis. 1985 Apr-Jun;29(2):408–415. [PubMed] [Google Scholar]
- Kleven S. H., Glisson J. R., Lin M. Y., Talkington F. D. Bacterins and vaccines for the control of Mycoplasma gallisepticum. Isr J Med Sci. 1984 Oct;20(10):989–991. [PubMed] [Google Scholar]
- McMartin D. A., Khan M. I., Farver T. B., Christie G. Delineation of the lateral spread of Mycoplasma gallisepticum infection in chickens. Avian Dis. 1987 Oct-Dec;31(4):814–819. [PubMed] [Google Scholar]
- Mohammed H. O., Yamamoto R., Carpenter T. E., Ortmayer H. B. Comparison of egg yolk and serum for the detection of Mycoplasma gallisepticum and M. synoviae antibodies by enzyme-linked immunosorbent assay. Avian Dis. 1986 Apr-Jun;30(2):398–408. [PubMed] [Google Scholar]
- Silim A., Venne D. Comparison of egg-yolk and serum antibody titers to four avian viruses by enzyme-linked immunosorbent assay using paired field samples. Avian Dis. 1989 Oct-Dec;33(4):643–648. [PubMed] [Google Scholar]
- Yoder H. W., Jr, Hopkins S. R. Efficacy of experimental inactivated mycoplasma gallisepticum oil-emulsion bacterin in egg-layer chickens. Avian Dis. 1985 Apr-Jun;29(2):322–334. [PubMed] [Google Scholar]
- Yoder H. W., Jr Nonspecific reactions to Mycoplasma serum plate antigens induced by inactivated poultry disease vaccines. Avian Dis. 1989 Jan-Mar;33(1):60–68. [PubMed] [Google Scholar]