Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Oct;30(10):2555–2559. doi: 10.1128/jcm.30.10.2555-2559.1992

Improvement of simultaneous detection of antibodies to Gag and envelope antigens of human T-lymphotropic virus type I by western immunoblot assay.

H Miyakoshi 1, M Sugimoto 1, H Igarashi 1, H Honda 1, R Fujino 1, M Mizukoshi 1
PMCID: PMC270478  PMID: 1400953

Abstract

To determine seropositivity for human T-lymphotropic virus type I (HTLV-I), we attempted to improve the detection system that uses antibody to HTLV-I Env in Western immunoblotting (WB) by adding an envelope glycoprotein (gp46) purified from the culture fluid of HTLV-I-producing cells by immunoaffinity chromatography and gel chromatography. In this WB, 177 of 179 serum samples showing seropositivity in an indirect immunofluorescence assay showed positive reactions to the gp46 envelope antigen as well as to p19, p24, and p53 Gag antigens. The remaining two samples showed negative reactions to p24. False-positive results were not found for 533 indirect immunofluorescence assay-negative serum samples, although one band to p19 or p24 was observed in 46 of the 533 samples. These 46 samples did not react to p53 and gp46, suggesting that these samples belonged to the indeterminate group in accordance with the criteria proposed by the World Health Organization. Therefore, this improved WB can be used for the confirmation of seropositivity.

Full text

PDF
2555

Images in this article

2557Image
on p.2557

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. W., Epstein J. S., Lee T. H., Lairmore M. D., Saxinger C., Kalyanaraman V. S., Slamon D., Parks W., Poiesz B. J., Pierik L. T. Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors. Blood. 1989 Nov 15;74(7):2585–2591. [PubMed] [Google Scholar]
  2. Aoki T., Miyakoshi H., Koide H., Yoshida T., Ishikawa H., Sugisaki Y., Mizukoshi M., Tamura K., Misawa H., Hamada C. Seroepidemiology of human T-lymphotropic retrovirus type I (HTLV-I) in residents of Niigata Prefecture, Japan. Comparative studies by indirect immunofluorescence microscopy and enzyme-linked immunosorbent assay. Int J Cancer. 1985 Mar 15;35(3):301–306. doi: 10.1002/ijc.2910350304. [DOI] [PubMed] [Google Scholar]
  3. Chen Y. M., Lee T. H., Samuel K. P., Okayama A., Tachibana N., Miyoshi I., Papas T. S., Essex M. Antibody reactivity to different regions of human T-cell leukemia virus type 1 gp61 in infected people. J Virol. 1989 Nov;63(11):4952–4957. doi: 10.1128/jvi.63.11.4952-4957.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coates S. R., Harris A. J., Parkes D. L., Smith C. M., Liu H. L., Akita R. W., Ferrer M. M., Sampson E. K., Brandis J. W., Sliwkowski M. X. Serological evaluation of Escherichia coli-expressed human T-cell leukemia virus type I env, gag p24, and tax proteins. J Clin Microbiol. 1990 Jun;28(6):1139–1142. doi: 10.1128/jcm.28.6.1139-1142.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fujii M., Sugamura K., Hinuma Y. A monoclonal antibody that defines p24, a core protein of adult T-cell leukemia virus, and its precursor. Gan. 1984 Jul;75(7):595–602. [PubMed] [Google Scholar]
  6. Fujino R., Kawato K., Ikeda M., Miyakoshi H., Mizukoshi M., Imai J. Improvement of gelatin particle agglutination test for detection of anti-HTLV-I antibody. Jpn J Cancer Res. 1991 Apr;82(4):367–370. doi: 10.1111/j.1349-7006.1991.tb01856.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gessain A., Barin F., Vernant J. C., Gout O., Maurs L., Calender A., de Thé G. Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis. Lancet. 1985 Aug 24;2(8452):407–410. doi: 10.1016/s0140-6736(85)92734-5. [DOI] [PubMed] [Google Scholar]
  8. Hauser S. L., Aubert C., Burks J. S., Kerr C., Lyon-Caen O., de The G., Brahic M. Analysis of human T-lymphotrophic virus sequences in multiple sclerosis tissue. Nature. 1986 Jul 10;322(6075):176–177. doi: 10.1038/322176a0. [DOI] [PubMed] [Google Scholar]
  9. Hino S., Sugiyama H., Doi H., Ishimaru T., Yamabe T., Tsuji Y., Miyamoto T. Breaking the cycle of HTLV-I transmission via carrier mothers' milk. Lancet. 1987 Jul 18;2(8551):158–159. doi: 10.1016/s0140-6736(87)92358-0. [DOI] [PubMed] [Google Scholar]
  10. Hinuma Y., Nagata K., Hanaoka M., Nakai M., Matsumoto T., Kinoshita K. I., Shirakawa S., Miyoshi I. Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6476–6480. doi: 10.1073/pnas.78.10.6476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ikeda M., Fujino R., Matsui T., Yoshida T., Komoda H., Imai J. A new agglutination test for serum antibodies to adult T-cell leukemia virus. Gan. 1984 Oct;75(10):845–848. [PubMed] [Google Scholar]
  12. Inaba S., Sato H., Okochi K., Fukada K., Takakura F., Tokunaga K., Kiyokawa H., Maeda Y. Prevention of transmission of human T-lymphotropic virus type 1 (HTLV-1) through transfusion, by donor screening with antibody to the virus. One-year experience. Transfusion. 1989 Jan;29(1):7–11. doi: 10.1046/j.1537-2995.1989.29189101168.x. [DOI] [PubMed] [Google Scholar]
  13. Kamihira S., Nakasima S., Oyakawa Y., Moriuti Y., Ichimaru M., Okuda H., Kanamura M., Oota T. Transmission of human T cell lymphotropic virus type I by blood transfusion before and after mass screening of sera from seropositive donors. Vox Sang. 1987;52(1-2):43–44. doi: 10.1111/j.1423-0410.1987.tb02987.x. [DOI] [PubMed] [Google Scholar]
  14. Kannagi M., Sugamura K., Sato H., Okochi K., Uchino H., Hinuma Y. Establishment of human cytotoxic T cell lines specific for human adult T cell leukemia virus-bearing cells. J Immunol. 1983 Jun;130(6):2942–2946. [PubMed] [Google Scholar]
  15. Karpas A., Kämpf U., Sidèn A., Koch M., Poser S. Lack of evidence for involvement of known human retroviruses in multiple sclerosis. Nature. 1986 Jul 10;322(6075):177–178. doi: 10.1038/322177a0. [DOI] [PubMed] [Google Scholar]
  16. Kinoshita K., Amagasaki T., Hino S., Doi H., Yamanouchi K., Ban N., Momita S., Ikeda S., Kamihira S., Ichimaru M. Milk-borne transmission of HTLV-I from carrier mothers to their children. Jpn J Cancer Res. 1987 Jul;78(7):674–680. [PubMed] [Google Scholar]
  17. Kiyokawa T., Yamaguchi K., Nishimura Y., Fukuoka N., Watanabe T., Takatsuki K. Western blot criteria for HTLV-I. Lancet. 1991 Aug 3;338(8762):312–312. doi: 10.1016/0140-6736(91)90450-4. [DOI] [PubMed] [Google Scholar]
  18. Koprowski H., DeFreitas E. C., Harper M. E., Sandberg-Wollheim M., Sheremata W. A., Robert-Guroff M., Saxinger C. W., Feinberg M. B., Wong-Staal F., Gallo R. C. Multiple sclerosis and human T-cell lymphotropic retroviruses. Nature. 1985 Nov 14;318(6042):154–160. doi: 10.1038/318154a0. [DOI] [PubMed] [Google Scholar]
  19. Kusuhara K., Sonoda S., Takahashi K., Tokugawa K., Fukushige J., Ueda K. Mother-to-child transmission of human T-cell leukemia virus type I (HTLV-I): a fifteen-year follow-up study in Okinawa, Japan. Int J Cancer. 1987 Dec 15;40(6):755–757. doi: 10.1002/ijc.2910400607. [DOI] [PubMed] [Google Scholar]
  20. Lillehoj E. P., Alexander S. S., Dubrule C. J., Wiktor S., Adams R., Tai C. C., Manns A., Blattner W. A. Development and evaluation of a human T-cell leukemia virus type I serologic confirmatory assay incorporating a recombinant envelope polypeptide. J Clin Microbiol. 1990 Dec;28(12):2653–2658. doi: 10.1128/jcm.28.12.2653-2658.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Maeda Y., Imai J., Kiyokawa H., Kanamura M., Hino S. Performance certification of gelatin particle agglutination assay for anti-HTLV-1 antibody: inconclusive positive results. Jpn J Cancer Res. 1989 Oct;80(10):915–919. doi: 10.1111/j.1349-7006.1989.tb01625.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Okochi K., Sato H., Hinuma Y. A retrospective study on transmission of adult T cell leukemia virus by blood transfusion: seroconversion in recipients. Vox Sang. 1984;46(5):245–253. doi: 10.1111/j.1423-0410.1984.tb00083.x. [DOI] [PubMed] [Google Scholar]
  23. Osame M., Usuku K., Izumo S., Ijichi N., Amitani H., Igata A., Matsumoto M., Tara M. HTLV-I associated myelopathy, a new clinical entity. Lancet. 1986 May 3;1(8488):1031–1032. doi: 10.1016/s0140-6736(86)91298-5. [DOI] [PubMed] [Google Scholar]
  24. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415–7419. doi: 10.1073/pnas.77.12.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sugamura K., Fujii M., Ueda S., Hinuma Y. Identification of a glycoprotein, gp21, of adult T cell leukemia virus by monoclonal antibody. J Immunol. 1984 Jun;132(6):3180–3184. [PubMed] [Google Scholar]
  26. Taguchi H., Sawada T., Fujishita M., Morimoto T., Niiya K., Miyoshi I. Enzyme-linked immunosorbent assay of antibodies to adult T-cell leukemia-associated antigens. Gan. 1983 Apr;74(2):185–187. [PubMed] [Google Scholar]
  27. Tajima K. The 4th nation-wide study of adult T-cell leukemia/lymphoma (ATL) in Japan: estimates of risk of ATL and its geographical and clinical features. The T- and B-cell Malignancy Study Group. Int J Cancer. 1990 Feb 15;45(2):237–243. doi: 10.1002/ijc.2910450206. [DOI] [PubMed] [Google Scholar]
  28. Tajima K., Tominaga S., Suchi T., Kawagoe T., Komoda H., Hinuma Y., Oda T., Fujita K. Epidemiological analysis of the distribution of antibody to adult T-cell leukemia-virus-associated antigen: possible horizontal transmission of adult T-cell leukemia virus. Gan. 1982 Dec;73(6):893–901. [PubMed] [Google Scholar]
  29. Tanaka Y., Inoi T., Tozawa H., Yamamoto N., Hinuma Y. A glycoprotein antigen detected with new monoclonal antibodies on the surface of human lymphocytes infected with human T-cell leukemia virus type-I (HTLV-I). Int J Cancer. 1985 Nov 15;36(5):549–555. doi: 10.1002/ijc.2910360506. [DOI] [PubMed] [Google Scholar]
  30. Tanaka Y., Koyanagi Y., Chosa T., Yamamoto N., Hinuma Y. Monoclonal antibody reactive with both p28 and p19 of adult T-cell leukemia virus-specific polypeptides. Gan. 1983 Jun;74(3):327–330. [PubMed] [Google Scholar]
  31. Tanaka Y., Lee B., Inoi T., Tozawa H., Yamamoto N., Hinuma Y. Antigens related to three core proteins of HTLV-I (p24, p19 and p15) and their intracellular localizations, as defined by monoclonal antibodies. Int J Cancer. 1986 Jan 15;37(1):35–42. doi: 10.1002/ijc.2910370107. [DOI] [PubMed] [Google Scholar]
  32. Uchiyama T., Yodoi J., Sagawa K., Takatsuki K., Uchino H. Adult T-cell leukemia: clinical and hematologic features of 16 cases. Blood. 1977 Sep;50(3):481–492. [PubMed] [Google Scholar]
  33. Verdier M., Denis F., Leonard G., Sangare A., Patillaud S., Prince-David M., Essex M. Comparison of immunofluorescence, particle agglutination, and enzyme immunoassays for detection of human T-cell leukemia virus type I antibody in African sera. J Clin Microbiol. 1990 Sep;28(9):1988–1993. doi: 10.1128/jcm.28.9.1988-1993.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yamamoto N., Schneider J., Hinuma Y., Hunsmann G. Adult T-cell leukemia-associated antigen (ATLA): detection of a glycoprotein in cell- and virus-free supernatant. Z Naturforsch C. 1982 Jul-Aug;37(7-8):731–732. doi: 10.1515/znc-1982-7-828. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES