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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Oct;30(10):2620–2624. doi: 10.1128/jcm.30.10.2620-2624.1992

Use of a modified shell vial technique to quantitate cytomegalovirus viremia in a population of solid-organ transplant recipients.

R S Buller 1, T C Bailey 1, N A Ettinger 1, M Keener 1, T Langlois 1, J P Miller 1, G A Storch 1
PMCID: PMC270488  PMID: 1328280

Abstract

A quantitative modification of the shell vial assay was used to investigate cytomegalovirus viremia in solid-organ transplant recipients. The level of viremia detected in 109 of 407 specimens ranged from 0.02 to 28 infectious foci per 100,000 leukocytes. By using a Poisson model, a technique was developed to determine 95% confidence limits for the measured levels of viremia. These confidence limits were used to determine the level of viremia that could be excluded by culturing a given number of cells. Longitudinal assessment of two transplant recipients revealed different patterns of viremia and demonstrated that significant disease sometimes occurred with low-level viremia. On the basis of the results of the studies, culture of at least 4 x 10(6) leukocytes is recommended for the sensitive detection of cytomegalovirus viremia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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