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. 2009 May 25;19(8):918–933. doi: 10.1093/glycob/cwp068

Fig. 6.

Fig. 6

Analysis of mutant TcOGNT2cat. Concentrated (50-fold) conditioned media (0.1% FBS) from L. tarentolae expressing native TcOGNT2cat, TcOGNT2cat(D234N), or TcOGNT2cat(D234A) were analyzed as described in Figure 4. (A) Western blot analysis using 1:500 anti-TcOGNT2 (bleed 7). Positions of TcOGNT2cat and an N-terminal fragment (TcOGNT2catN; see the text) are indicated. (B) Transferase and UDP-GlcNAc hydrolysis activities. P10, normal parental strain; wt, P10 expressing wild-type TcOGNT2cat; D234A and D234N, point mutations at Asp234. Similar results were obtained in an independent trial.