Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Oct;30(10):2714–2716. doi: 10.1128/jcm.30.10.2714-2716.1992

Comparison of the Sceptor Pseudomonas Plus MIC Panel with agar dilution for susceptibility testing of Pseudomonas aeruginosa.

L F Joyce 1, K Stockman 1, J Downes 1, J H Andrew 1
PMCID: PMC270504  PMID: 1400973

Abstract

The antimicrobial susceptibilities of 100 clinical isolates of Pseudomonas aeruginosa to gentamicin, amikacin, tobramycin, ticarcillin, piperacillin, and ceftazidime were determined by using the Sceptor system (BBL Microbiology Systems, Cockeysville, Md.), and the results were compared with those obtained using the National Committee for Clinical Laboratory Standards reference agar dilution method. Excellent correlation was observed for the aminoglycosides, with greater than 95% agreement within 1 doubling dilution of the reference agar dilution MIC, while ticarcillin and piperacillin showed lower percent agreement values of 91 and 88%, respectively.

Full text

PDF
2714

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry A. L., Miller G. H., Thornsberry C., Hare R. S., Jones R. N., Lorber R. R., Ferraresi R., Cramer C. Influence of cation supplements on activity of netilmicin against Pseudomonas aeruginosa in vitro and in vivo. Antimicrob Agents Chemother. 1987 Oct;31(10):1514–1518. doi: 10.1128/aac.31.10.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chow A. W., Wong J., Bartlett K. H., Shafran S. D., Stiver H. G. Cross-resistance of Pseudomonas aeruginosa to ciprofloxacin, extended-spectrum beta-lactams, and aminoglycosides and susceptibility to antibiotic combinations. Antimicrob Agents Chemother. 1989 Aug;33(8):1368–1372. doi: 10.1128/aac.33.8.1368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark R. B., Sanders C. C., Pakiz C. B., Hostetter M. K. Aminoglycoside resistance among Pseudomonas aeruginosa isolates with an unusual disk diffusion antibiogram. Antimicrob Agents Chemother. 1988 May;32(5):689–692. doi: 10.1128/aac.32.5.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eng R. H., Smith S. M., Cherubin C. Inoculum effect of new beta-lactam antibiotics on Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1984 Jul;26(1):42–47. doi: 10.1128/aac.26.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Garrod L. P., Waterworth P. M. Effect of medium composition on the apparent sensitivity of Pseudomonas aeruginosa to gentamicin. J Clin Pathol. 1969 Sep;22(5):534–538. doi: 10.1136/jcp.22.5.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jones R. N., Thornsberry C., Barry A. L., Gavan T. L. Evaluation of the Sceptor microdilution antibiotic susceptibility testing system: a collaborative investigation. J Clin Microbiol. 1981 Jan;13(1):184–194. doi: 10.1128/jcm.13.1.184-194.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Joyce L. F., Downes J., Stockman K., Andrew J. H. Comparison of five methods, including the PDM Epsilometer test (E test), for antimicrobial susceptibility testing of Pseudomonas aeruginosa. J Clin Microbiol. 1992 Oct;30(10):2709–2713. doi: 10.1128/jcm.30.10.2709-2713.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kenny M. A., Pollock H. M., Minshew B. H., Casillas E., Schoenknecht F. D. Cation components of Mueller-Hinton agar affecting testing of Pseudomonas aeruginosa susceptibility to gentamicin. Antimicrob Agents Chemother. 1980 Jan;17(1):55–62. doi: 10.1128/aac.17.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Livermore D. M., Wood M. J. Mechanisms and clinical significance of resistance to new beta-lactam antibiotics. Br J Hosp Med. 1990 Oct;44(4):252-8, 261-3. [PubMed] [Google Scholar]
  10. Pollock H. M., Minshew B. H., Kenny M. A., Schoenknecht F. D. Effect of different lots of Mueller-Hinton agar on the interpretation of the gentamicin susceptibility of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1978 Sep;14(3):360–367. doi: 10.1128/aac.14.3.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reller L. B., Schoenknecht F. D., Kenny M. A., Sherris J. C. Antibiotic susceptibility testing of Pseudomonas aeruginosa: selection of a control strain and criteria for magnesium and calcium content in media. J Infect Dis. 1974 Nov;130(5):454–463. doi: 10.1093/infdis/130.5.454. [DOI] [PubMed] [Google Scholar]
  12. Staneck J. L., Glenn S., DiPersio J. R., Leist P. A. Wide variability in Pseudomonas aeruginosa aminoglycoside results among seven susceptibility testing procedures. J Clin Microbiol. 1989 Oct;27(10):2277–2285. doi: 10.1128/jcm.27.10.2277-2285.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Woolfrey B. F., Fox J. M., Quall C. O. Comparison of minimum inhibitory concentration values determined by three antimicrobic dilution methods for Pseudomonas aeruginosa. Am J Clin Pathol. 1981 Jan;75(1):39–44. doi: 10.1093/ajcp/75.1.39. [DOI] [PubMed] [Google Scholar]
  14. Woolfrey B. F., Lally R. T., Quall C. O. Comparative evaluation of the micro-media system, sceptor, and MIC-2000 microdilution methods for testing Pseudomonas aeruginosa against gentamicin, tobramycin, and amikacin. J Clin Microbiol. 1983 Feb;17(2):312–316. doi: 10.1128/jcm.17.2.312-316.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES