Abstract
A method of discriminating between strains of Mycobacterium tuberculosis by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins combined with a sensitive silver stain is described. Thirty-five isolates of M. tuberculosis and five isolates from other species of Mycobacterium were examined, including serial isolates from the same patients and isolates from a small cluster of hospital cases. Different species of Mycobacterium were clearly distinguished, and within the species M. tuberculosis, different fingerprints were obtained, allowing discrimination of some strains from different patients. The reproducibility and discrimination of the technique are discussed.
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- De Jong A., Hoentjen A. H., Van Der Zanden A. G. A rapid method for identification of Mycobacterium species by polyacrylamide gel electrophoresis of soluble cell proteins. J Med Microbiol. 1991 Jan;34(1):1–5. doi: 10.1099/00222615-34-1-1. [DOI] [PubMed] [Google Scholar]
- Engel H. W. Mycobacteriophages and phage typing. Ann Microbiol (Paris) 1978 Jan;129(1):75–90. [PubMed] [Google Scholar]
- Hermans P. W., van Soolingen D., Dale J. W., Schuitema A. R., McAdam R. A., Catty D., van Embden J. D. Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis. J Clin Microbiol. 1990 Sep;28(9):2051–2058. doi: 10.1128/jcm.28.9.2051-2058.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Millership S. E., Want S. V. Typing of Aeromonas species by protein fingerprinting: comparison of radiolabelling and silver staining for visualising proteins. J Med Microbiol. 1989 May;29(1):29–32. doi: 10.1099/00222615-29-1-29. [DOI] [PubMed] [Google Scholar]
- Shoemaker S. A., Fisher J. H., Jones W. D., Jr, Scoggin C. H. Restriction fragment analysis of chromosomal DNA defines different strains of Mycobacterium tuberculosis. Am Rev Respir Dis. 1986 Aug;134(2):210–213. doi: 10.1164/arrd.1986.134.2.210. [DOI] [PubMed] [Google Scholar]
- Tabaqchali S., O'Farrell S., Holland D., Silman R. Method for the typing of Clostridium difficile based on polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins. J Clin Microbiol. 1986 Jan;23(1):197–198. doi: 10.1128/jcm.23.1.197-198.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Véron M. Sur un critère de calcul du meilleur niveau de coupure d'un dendrogramme de classification hiérarchique. Ann Microbiol (Paris) 1974 Jul-Aug;125B(1):29–44. [PubMed] [Google Scholar]