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. 1992 Dec;30(12):3200–3205. doi: 10.1128/jcm.30.12.3200-3205.1992

Comparison of nine antigen detection kits for diagnosis of urogenital infections due to Chlamydia psittaci in koalas.

M M Wood 1, P Timms 1
PMCID: PMC270626  PMID: 1452703

Abstract

Chlamydia psittaci is the major cause of infectious disease in the koala (Phascolarctos cinereus). It causes four disease syndromes in the koala, namely, conjunctivitis, rhinitis, cystitis, and infertility (females only). Diagnosis of chlamydial infections in koalas relies primarily on isolation of the organism in cell culture. Serology has generally not been useful, and little use has previously been made of the commercially available antigen detection kits. We examined the sensitivity, specificity, and usefulness of three direct fluorescent-antibody kits (Vet-IF [Cell Labs], IMAGEN [Celltech], Chlamydia-Direct IF [Bio Merieux]) and six antigen detection enzyme-linked immunosorbent assay (ELISA) kits (Clearview [Unipath], Surecell [Kodak], Pathfinder [Kallestad], Chlamydia-EIA [Pharmacia], Chlamydiazyme [Abbott], IDEIA [Celltech]) for the detection of urogenital infections in koalas. Laboratory studies showed that the direct fluorescent-antibody kits were the least sensitive in this case and did not detect fewer than 10(4) elementary bodies per ml, while most ELISA kits detected between 130 and 600 elementary bodies per ml. Field study results showed that the Clearview kit was the most sensitive (91%) compared with the IDEIA (88%) and the Surecell (73%) kits. All three kits were more sensitive than cell culture (36%), highlighting viability loss problems that occur during transport. This study showed that the Clearview kit is sensitive, specific, and easy to use for the detection of type II (urogenital) C. psittaci from koalas in the field and warrants further evaluation.

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Selected References

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  1. Brown A. S., Grice R. G. Isolation of Chlamydia psittaci from koalas (Phascolarctos cinereus). Aust Vet J. 1984 Dec;61(12):413–413. doi: 10.1111/j.1751-0813.1984.tb07182.x. [DOI] [PubMed] [Google Scholar]
  2. Bäckman M., Rudén A. K., Ringertz O., Sanström E. G. Evaluation of a commercial enzyme immunoassay versus culture for the detection of Chlamydia trachomatis. Eur J Clin Microbiol Infect Dis. 1989 Sep;8(9):778–782. doi: 10.1007/BF02185844. [DOI] [PubMed] [Google Scholar]
  3. Canfield P. J., Love D. N., Mearns G., Farram E. Evaluation of an immunofluorescence test on direct smears of conjunctival and urogenital swabs taken from koalas for the detection of Chlamydia psittaci. Aust Vet J. 1991 May;68(5):165–167. doi: 10.1111/j.1751-0813.1991.tb03170.x. [DOI] [PubMed] [Google Scholar]
  4. Cles L. D., Bruch K., Stamm W. E. Staining characteristics of six commercially available monoclonal immunofluorescence reagents for direct diagnosis of Chlamydia trachomatis infections. J Clin Microbiol. 1988 Sep;26(9):1735–1737. doi: 10.1128/jcm.26.9.1735-1737.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cockram F. A., Jackson A. R. Letter: Isolation of a Chlamydia from cases of keratoconjunctivitis in koalas. Aust Vet J. 1974 Feb;50(2):82–83. doi: 10.1111/j.1751-0813.1974.tb05265.x. [DOI] [PubMed] [Google Scholar]
  6. Dunlop E. M., Goh B. T., Darougar S., Woodland R. Triple-culture tests for diagnosis of chlamydial infection of the female genital tract. Sex Transm Dis. 1985 Apr-Jun;12(2):68–71. doi: 10.1097/00007435-198504000-00003. [DOI] [PubMed] [Google Scholar]
  7. Ehret J. M., Judson F. N. Genital Chlamydia infections. Clin Lab Med. 1989 Sep;9(3):481–500. [PubMed] [Google Scholar]
  8. Girjes A. A., Hugall A. F., Timms P., Lavin M. F. Two distinct forms of Chlamydia psittaci associated with disease and infertility in Phascolarctos cinereus (koala). Infect Immun. 1988 Aug;56(8):1897–1900. doi: 10.1128/iai.56.8.1897-1900.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Girjes A. A., Weigler B. J., Hugall A. F., Carrick F. N., Lavin M. F. Detection of Chlamydia psittaci in free-ranging koalas (Phascolarctos cinereus): DNA hybridization and immuno-slot blot analyses. Vet Microbiol. 1989 Nov;21(1):21–30. doi: 10.1016/0378-1135(89)90015-1. [DOI] [PubMed] [Google Scholar]
  10. Grice R. G., Brown A. S. A tissue culture procedure for the isolation of Chlamydia psittaci from koalas (Phascolarctos cinereus). Aust J Exp Biol Med Sci. 1985 Jun;63(Pt 3):283–286. doi: 10.1038/icb.1985.31. [DOI] [PubMed] [Google Scholar]
  11. Hall C. J., Nelder C. Comparison of three non-culture techniques for detection of Chlamydia trachomatis in genital tract specimens. Eur J Clin Microbiol Infect Dis. 1989 Oct;8(10):866–870. doi: 10.1007/BF01963772. [DOI] [PubMed] [Google Scholar]
  12. Hammerschlag M. R., Gelling M., Roblin P. M., Worku M. Comparison of Kodak Surecell Chlamydia Test Kit with culture for the diagnosis of chlamydial conjunctivitis in infants. J Clin Microbiol. 1990 Jun;28(6):1441–1442. doi: 10.1128/jcm.28.6.1441-1442.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hipp S. S., Han Y., Murphy D. Assessment of enzyme immunoassay and immunofluorescence tests for detection of Chlamydia trachomatis. J Clin Microbiol. 1987 Oct;25(10):1938–1943. doi: 10.1128/jcm.25.10.1938-1943.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jones R. B., Katz B. P., van der Pol B., Caine V. A., Batteiger B. E., Newhall W. J. Effect of blind passage and multiple sampling on recovery of Chlamydia trachomatis from urogenital specimens. J Clin Microbiol. 1986 Dec;24(6):1029–1033. doi: 10.1128/jcm.24.6.1029-1033.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lipkin E. S., Moncada J. V., Shafer M. A., Wilson T. E., Schachter J. Comparison of monoclonal antibody staining and culture in diagnosing cervical chlamydial infection. J Clin Microbiol. 1986 Jan;23(1):114–117. doi: 10.1128/jcm.23.1.114-117.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McColl K. A., Martin R. W., Gleeson L. J., Handasyde K. A., Lee A. K. Chlamydia infection and infertility in the female koala (Phascolarctos cinereus). Vet Rec. 1984 Dec 22;115(25-26):655–655. doi: 10.1136/vr.115.25-26.655. [DOI] [PubMed] [Google Scholar]
  17. Munday P. E., Carder J. M., Hanna N. F., Taylor-Robinson D. Is one swab enough to detect chlamydial infection of the cervix? Br J Vener Dis. 1984 Dec;60(6):384–386. doi: 10.1136/sti.60.6.384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schachter J. Immunodiagnosis of sexually transmitted disease. Yale J Biol Med. 1985 Sep-Oct;58(5):443–452. [PMC free article] [PubMed] [Google Scholar]
  19. Smith J. W., Rogers R. E., Katz B. P., Brickler J. F., Lineback P. L., Van der Pol B., Jones R. B. Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics. J Clin Microbiol. 1987 May;25(5):868–872. doi: 10.1128/jcm.25.5.868-872.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stamm W. E. Diagnosis of Chlamydia trachomatis genitourinary infections. Ann Intern Med. 1988 May;108(5):710–717. doi: 10.7326/0003-4819-108-5-710. [DOI] [PubMed] [Google Scholar]
  21. Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]
  22. Taylor-Robinson D., Thomas B. J., Osborn M. F. Evaluation of enzyme immunoassay (Chlamydiazyme) for detecting Chlamydia trachomatis in genital tract specimens. J Clin Pathol. 1987 Feb;40(2):194–199. doi: 10.1136/jcp.40.2.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Uyeda C. T., Welborn P., Ellison-Birang N., Shunk K., Tsaouse B. Rapid diagnosis of chlamydial infections with the MicroTrak direct test. J Clin Microbiol. 1984 Nov;20(5):948–950. doi: 10.1128/jcm.20.5.948-950.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weigler B. J., Baldock F. C., Girjes A. A., Carrick F. N., Lavin M. F. Evaluation of an enzyme immunoassay test for the diagnosis of Chlamydia psittaci infection in free-ranging koalas (Phascolarctos cinereus) in southeastern Queensland, Australia. J Wildl Dis. 1988 Apr;24(2):259–263. doi: 10.7589/0090-3558-24.2.259. [DOI] [PubMed] [Google Scholar]
  25. Weigler B. J., Girjes A. A., White N. A., Kunst N. D., Carrick F. N., Lavin M. F. Aspects of the epidemiology of Chlamydia psittaci infection in a population of koalas (Phascolarctos cinereus) in southeastern Queensland, Australia. J Wildl Dis. 1988 Apr;24(2):282–291. doi: 10.7589/0090-3558-24.2.282. [DOI] [PubMed] [Google Scholar]

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