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. Author manuscript; available in PMC: 2009 Jul 7.
Published in final edited form as: Proteomics. 2009 Mar;9(5):1142–1151. doi: 10.1002/pmic.200800404

Figure 1.

Figure 1

The experimental protocol followed: Pb infected blood was harvested from ∼25 rats and ookinetes cultured. Ookinete purification by nycodenz gradient was followed by lysis by sonication. Remaining intact cells and nuclei were removed by centrifugation at 800×g. The post-nuclear fraction was separated on a sucrose gradient, 1 mL fractions were removed for analysis by Western blot. The 1.1 M sucrose fraction was characterised by TEM, abundant proteins were sequenced by LC-MS/MS and a global survey was conducted by MudPIT shotgun proteomics. The localisation of selected proteins were analysed by IFA.