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. Author manuscript; available in PMC: 2009 Jul 7.
Published in final edited form as: Biochemistry. 2008 May 14;47(24):6361–6369. doi: 10.1021/bi800657p

FIGURE 2. SDS-polyacrylamide gel electrophoresis analysis of the purified wild-type and mutant SppA.

FIGURE 2

The purified enzymes were run on 12% SDS-polyacrylamide gel and stained with Coomassie Blue R-250 M:Marker 1:WT 2:S374A 3:S402A 4:S409A 5:S431A 6:R496K 7:R496A 8:K366A 9:K397A 10:H451A 11:H497A 12:H510A 13: K364A 14: K535A 15:K486A 16:K505A 17:K518A 18:K541A 19:K209A 20 D524N