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. 1983 Jul;18(1):56–62. doi: 10.1128/jcm.18.1.56-62.1983

Modified oxidation-fermentation medium for detection of acid production from carbohydrates by Neisseria spp. and Branhamella catarrhalis.

J S Knapp, K K Holmes
PMCID: PMC270744  PMID: 6885992

Abstract

A modified oxidation-fermentation medium was developed as a practical medium for highly sensitive and specific detection of acid production from carbohydrates by Neisseria spp. and Branhamella catarrhalis. A total of 756 strains representing 17 Neisseria spp. and Branhamella catarrhalis were tested in this medium, in which the protein concentration was reduced relative to the carbohydrate concentration, phenol red was substituted for bromthymol blue at a low concentration, and the initial pH was adjusted to 7.2. Sugar utilization patterns were consistent with published results and with other cultural and biochemical characteristics for these species. The reactions obtained using this medium were qualitatively better and more reproducible than those obtained in cystine-Trypticase agar (BBL Microbiology Systems, Cockeysville, Md.) medium.

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Selected References

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  1. BERGER U., BRUNHOEBER H. [Neisseria flava (Bergey et al., 1923): species or variety?]. Z Hyg Infektionskr. 1961;148:39–44. [PubMed] [Google Scholar]
  2. Baron E. S., Saz A. K. Effect of types of media on the production of acid from glucose by so-called glucose-negative strains of Neisseria gonorrhoeae. J Clin Microbiol. 1976 Mar;3(3):330–333. doi: 10.1128/jcm.3.3.330-333.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown W. J. A comparison of three fermentation methods for the confirmation of Neisseria gonorrhoeae. Health Lab Sci. 1976 Jan;13(1):54–58. [PubMed] [Google Scholar]
  4. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Morello J. A., Lerner S. A., Bohnhoff M. Characteristics of atypical Neisseria gonorrhoeae from disseminated and localized infections. Infect Immun. 1976 May;13(5):1510–1516. doi: 10.1128/iai.13.5.1510-1516.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pollock H. M. Evaluation of methods for the rapid identification of Neisseria gonorrhoeae in a routine clinical laboratory. J Clin Microbiol. 1976 Jul;4(1):19–21. doi: 10.1128/jcm.4.1.19-21.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. VERON M., THIBAULT P., SECOND L. [Neisseria mucosa (Diplococcus mucosus Lingelsheim). I. Bacteriological description and study of its pathogenicity]. Ann Inst Pasteur (Paris) 1959 Oct;97:497–510. [PubMed] [Google Scholar]
  8. VERON M., THIBAULT P., SECOND L. [Neisseria mucosa (Diplococcus mucosus Lingelsheim). II. Antigenic studies and classification]. Ann Inst Pasteur (Paris) 1961 Feb;100:166–179. [PubMed] [Google Scholar]
  9. Vera H. D. A Simple Medium for Identification and Maintenance of the Gonococcus and Other Bacteria. J Bacteriol. 1948 Apr;55(4):531–536. doi: 10.1128/jb.55.4.531-536.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. WHITE L. A., KELLOGG D. S., Jr NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD. Appl Microbiol. 1965 Mar;13:171–174. doi: 10.1128/am.13.2.171-174.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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