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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Aug;18(2):300–304. doi: 10.1128/jcm.18.2.300-304.1983

Comparative recovery of bacteria and yeasts from lysis-centrifugation and a conventional blood culture system.

T E Kiehn, B Wong, F F Edwards, D Armstrong
PMCID: PMC270795  PMID: 6352732

Abstract

Blood cultures obtained with a lysis-centrifugation (L-C) system and a conventional two-bottle broth system were compared for the recovery of bacteria and yeasts from 7,000 cultures. The L-C system recovered significantly more total organisms, Escherichia coli, and Candida spp. and detected more patients with bacteremia and fungemia due to members of the family Enterobacteriaceae and yeasts. The broth system recovered significantly more streptococci and detected significantly more low-level Pseudomonas bacteremias. Polymicrobic bacteremia and fungemia were detected equally well by either culture system. Aerobic organisms grew equally well on blood, chocolate, or brain heart infusion agar plates used for L-C inoculation. A total of 82% of colony counts measured no more than 10 CFU/ml of blood, and it was at these low levels that enhanced detection of organisms by either system was observed. The L-C system isolated organisms and detected yeasts more rapidly than did the broth system. Contaminants occurred in 8.2% of L-C cultures and 1.9% of broth cultures. Low colony counts on L-C plates occurred for both Staphylococcus epidermidis contamination and septicemia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dorn G. L., Burson G. G., Haynes J. R. Blood culture technique based on centrifugation: clinical evaluation. J Clin Microbiol. 1976 Mar;3(3):258–263. doi: 10.1128/jcm.3.3.258-263.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dorn G. L., Haynes J. R., Burson G. G. Blood culture technique based on centrifugation: developmental phase. J Clin Microbiol. 1976 Mar;3(3):251–257. doi: 10.1128/jcm.3.3.251-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dorn G. L., Land G. A., Wilson G. E. Improved blood culture technique based on centrifugation: clinical evaluation. J Clin Microbiol. 1979 Mar;9(3):391–396. doi: 10.1128/jcm.9.3.391-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dorn G. L., Smith K. New centrifugation blood culture device. J Clin Microbiol. 1978 Jan;7(1):52–54. doi: 10.1128/jcm.7.1.52-54.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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