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. 1983 Aug;18(2):380–383. doi: 10.1128/jcm.18.2.380-383.1983

Evaluation of sodium polyanethanol sulfonate as a blood culture additive for recovery of Salmonella typhi and Salmonella paratyphi A.

J Escamilla, L T Santiago, C V Uylangco, J H Cross
PMCID: PMC270809  PMID: 6311874

Abstract

A total of 640 blood specimens from patients in an area endemic for enteric fever were cultured in parallel in tryptic soy broth with and without sodium polyanethanol sulfonate (SPS). A total of 95 specimens were positive for Salmonella spp., 54 for Salmonella typhi, and 41 for Salmonella paratyphi A in one or both bottles of a set. Significantly higher rates of recovery were obtained from the SPS-containing medium (P less than 0.01) upon subculturing blindly at 24 h and 3 days of incubation. Subcultures performed at 7, 14, and 21 days also yielded a greater number of positive cultures with SPS than without it, although the differences between the two media were not significant (P greater than 0.05). Neither of the media yielded 100% of the positive cultures. Moreover, even if the results of the two media were combined, 34 and 19% of the isolates would have been missed if the specimens had not been incubated to 14 and 21 days, respectively. The data indicate that SPS aids in early recovery of S. typhi and S. paratyphi A from blood cultures, and additionally, that under the conditions used in the study, incubation beyond a 1-week period is required for efficient isolation of these organisms from blood.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belding M. E., Klebanoff S. J. Effect of sodium polyanetholesulfonate on antimicrobial systems in blood. Appl Microbiol. 1972 Nov;24(5):691–698. doi: 10.1128/am.24.5.691-698.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CAVALLO G., PONTIERI G., IMPERATO S. Interaction between "liquoid" (sodiumpolyanetholsulphonate) and lysozyme in the immune haemolytic reaction. Experientia. 1963 Jan 15;19:36–37. doi: 10.1007/BF02135347. [DOI] [PubMed] [Google Scholar]
  3. Edberg S. C., Bottenbley C. J., Gam K. Use of sodium polyanethol sulfonate to selectively inhibit aminoglycoside and polymyxin antibiotics in a rapid blood level antibiotic assay. Antimicrob Agents Chemother. 1976 Mar;9(3):414–417. doi: 10.1128/aac.9.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eng J. Effect of sodium polyanethol sulfonate in blood cultures. J Clin Microbiol. 1975 Feb;1(2):119–123. doi: 10.1128/jcm.1.2.119-123.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eng J., Iveland H. Inhibitory effect in vitro of sodium polyanethol sulfonate on the growth of Neisseria meningitidis. J Clin Microbiol. 1975 May;1(5):444–447. doi: 10.1128/jcm.1.5.444-447.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finegold S. M., Ziment I., White M. L., Winn W. R., Carter W. T. Evaluation of polyanethol sulfonate (liquoid) in blood cultures. Antimicrob Agents Chemother (Bethesda) 1967;7:692–696. [PubMed] [Google Scholar]
  7. Gulati P. D., Saxena S. N., Gupta P. S., Chuttani H. K. Changing pattern of typhoid fever. Am J Med. 1968 Oct;45(4):544–548. doi: 10.1016/0002-9343(68)90170-8. [DOI] [PubMed] [Google Scholar]
  8. Kaye D., Palmieri M., Eyckmans L., Rocha H., Hook E. W. Comparison of bile and trypticase soy broth for isolation Salmonella from blood. Am J Clin Pathol. 1966 Sep;46(3):408–410. doi: 10.1093/ajcp/46.3_ts.408. [DOI] [PubMed] [Google Scholar]
  9. Lowrance B. L., Traub W. H. Inactivation of the bactericidal activity of human serum by liquoid (sodium polyanetholsulfonate). Appl Microbiol. 1969 Jun;17(6):839–842. doi: 10.1128/am.17.6.839-842.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Minkus R., Moffet H. L. Detection of bacteremia in children with sodium polyanethol sulfonate: a prospective clinical study. Appl Microbiol. 1971 Nov;22(5):805–808. doi: 10.1128/am.22.5.805-808.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rosner R. Evaluation of four blood culture systems using parallel culture methods. Appl Microbiol. 1974 Aug;28(2):245–247. doi: 10.1128/am.28.2.245-247.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SHAW A. B., MACKAY H. A. F. Factors influencing the results of blood culture in enteric fever. J Hyg (Lond) 1951 Jun-Sep;49(2-3):315–323. doi: 10.1017/s0022172400044181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WATSON K. C. Culture media for Salmonella typhi and the effect of complement destroying agents. J Lab Clin Med. 1956 Feb;47(2):329–332. [PubMed] [Google Scholar]
  14. WATSON K. C. Isolation of Salmonella typhi from the blood stream. J Lab Clin Med. 1955 Jul;46(1):128–134. [PubMed] [Google Scholar]
  15. Wicks A. C., Holmes G. S., Davidson L. Endemic typhoid fever. A diagnostic pitfall. Q J Med. 1971 Jul;40(159):341–354. [PubMed] [Google Scholar]

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