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. 1983 Aug;18(2):400–407. doi: 10.1128/jcm.18.2.400-407.1983

Presumptive identification of Vibrio species with H antiserum.

M G Tassin, R J Siebeling, N C Roberts, A D Larson
PMCID: PMC270813  PMID: 6619289

Abstract

Species in the genus Vibrio exhibit flagellar (H) antigens unique to the species. Thus, species-specific H antiserum could be a valuable reagent with which to screen serologically large numbers of Vibrio isolates. Antisera against V. cholerae, V. fluvialis, V. anguillarum, V. metschnikovii, V. parahaemolyticus, V. alginolyticus, and V. vulnificus H antigens was produced in rabbits by repeated injections of Formalin-preserved whole cells. Anti-O activity and anti-H activity against common H antigens was absorbed from each antiserum, V. fluvialis was shown to possess an H antigen unique to the species and also to share minor H antigens with V. cholerae, V. metschnikovii, and V. anguillarum. V. vulnificus also exhibits a species-unique H antigen. A comprehensive serological screening system based on species-specific H antiserum was developed to identify pathogenic Vibrio species one step beyond primary isolation. Vibrio species were correctly identified with accuracies ranging from 93 to 100%. Some isolates were either nonmotile or poorly so and thus did not flocculate in H antiserum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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