Abstract
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been used previously to characterize the flagellin of Pseudomonas aeruginosa. flFlagella from several other clinically important species of pseudomonads have been characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their molecular weights have been found to vary among species as follows: P. maltophilia B69 Fla, 33,000; P. stutzeri HEW, 55,000; P. aeruginosa M-2, 53,000. The flagella of P. cepacia strains were divided into two groups based on molecular weight. Type I had a molecular weight of 31,000. The molecular weight of type II was in the range of 44,000 to 46,000. Serologically, type I is a homologous group, whereas type II is a heterologous group. The two flagellin types of P. cepacia appear to be analogous to the two major flagellin types of P. aeruginosa. Characterization of P. cepacia strains by flagellin types may serve as a molecular epidemiological tool.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ansorg R. Flagellaspezifisches H-Antigenschema von Pseudomonas aeruginosa. Zentralbl Bakteriol Orig A. 1978 Nov;242(2):228–238. [PubMed] [Google Scholar]
- Ansorg R., Weber R., Kleinmaier H. Untersuchungen zur serologischen Typisierung von Pseudomonas aeruginosa mit der indirekten Immunofluoreszenztechnik. Zentralbl Bakteriol Orig A. 1977;237(2-3):280–296. [PubMed] [Google Scholar]
- Craven R. C., Montie T. C. Motility and chemotaxis of three strains of Pseudomonas aeruginosa used for virulence studies. Can J Microbiol. 1981 Apr;27(4):458–460. doi: 10.1139/m81-070. [DOI] [PubMed] [Google Scholar]
- Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
- Farmer J. J., 3rd Pseudomonas in the hospital. Hosp Pract. 1976 Feb;11(2):63–70. doi: 10.1080/21548331.1976.11706501. [DOI] [PubMed] [Google Scholar]
- Holder I. A., Wheeler R., Montie T. C. Flagellar preparations from Pseudomonas aeruginosa: animal protection studies. Infect Immun. 1982 Jan;35(1):276–280. doi: 10.1128/iai.35.1.276-280.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lagenaur C., Agabian N. Physical characterization of Caulobacter crescentus flagella. J Bacteriol. 1976 Oct;128(1):435–444. doi: 10.1128/jb.128.1.435-444.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mocca L. F., Frasch C. E. Sodium dodecyl sulfate-polyacrylamide gel typing system for characterization of Neisseria meningitidis isolates. J Clin Microbiol. 1982 Aug;16(2):240–244. doi: 10.1128/jcm.16.2.240-244.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montie T. C., Craven R. C., Holder I. A. Flagellar preparations from Pseudomonas aeruginosa: isolation and characterization. Infect Immun. 1982 Jan;35(1):281–288. doi: 10.1128/iai.35.1.281-288.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montie T. C., Doyle-Huntzinger D., Craven R. C., Holder I. A. Loss of virulence associated with absence of flagellum in an isogenic mutant of Pseudomonas aeruginosa in the burned-mouse model. Infect Immun. 1982 Dec;38(3):1296–1298. doi: 10.1128/iai.38.3.1296-1298.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moulton R. C., Montie T. C. Chemotaxis by Pseudomonas aeruginosa. J Bacteriol. 1979 Jan;137(1):274–280. doi: 10.1128/jb.137.1.274-280.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]