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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Sep;18(3):469–471. doi: 10.1128/jcm.18.3.469-471.1983

Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood.

J Bille, L Stockman, G D Roberts, C D Horstmeier, D M Ilstrup
PMCID: PMC270835  PMID: 6630434

Abstract

A lysis-centrifugation system (Isolator; E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with a biphasic brain heart infusion (BHI) medium in a prospective study of 5,125 fungal blood cultures. The Isolator recovered 90.3% of the positive cultures, compared with 63.4% recovered by the biphasic BHI medium. Overall, the detection of fungemia was increased 36.6% by the Isolator. Mean recovery times for yeasts were 2.12 and 4.90 days for the Isolator and BHI bottles, respectively. Cultures of Histoplasma capsulatum required 8.0 and 24.14 days for recovery by the Isolator and BHI bottles, respectively. The Isolator provided a more rapid and sensitive means of detecting organisms associated with fungemia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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