Abstract
A lysis-centrifugation system (Isolator; E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with a biphasic brain heart infusion (BHI) medium in a prospective study of 5,125 fungal blood cultures. The Isolator recovered 90.3% of the positive cultures, compared with 63.4% recovered by the biphasic BHI medium. Overall, the detection of fungemia was increased 36.6% by the Isolator. Mean recovery times for yeasts were 2.12 and 4.90 days for the Isolator and BHI bottles, respectively. Cultures of Histoplasma capsulatum required 8.0 and 24.14 days for recovery by the Isolator and BHI bottles, respectively. The Isolator provided a more rapid and sensitive means of detecting organisms associated with fungemia.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bille J., Roberts G. D., Washington J. A., 2nd Retrospective comparison of three blood culture media for the recovery of yeasts from clinical specimens. Eur J Clin Microbiol. 1983 Feb;2(1):22–25. doi: 10.1007/BF02019918. [DOI] [PubMed] [Google Scholar]
- Blazevic D. J., Stemper J. E., Matsen J. M. Effect of aerobic and anaerobic atmospheres on isolation of organisms from blood cultures. J Clin Microbiol. 1975 Feb;1(2):154–156. doi: 10.1128/jcm.1.2.154-156.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dorn G. L., Land G. A., Wilson G. E. Improved blood culture technique based on centrifugation: clinical evaluation. J Clin Microbiol. 1979 Mar;9(3):391–396. doi: 10.1128/jcm.9.3.391-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gantz N. M., Medeiros A. A., Swain J. L., O'Brien T. F. Vacuum blood-culture bottles inhibiting growth of Candida and fostering growth of Bacteroides. Lancet. 1974 Nov 16;2(7890):1174–1176. doi: 10.1016/s0140-6736(74)90813-7. [DOI] [PubMed] [Google Scholar]
- Kiehn T. E., Capitolo C., Mayo J. B., Armstrong D. Comparative recovery of fungi from biphasic and conventional blood culture media. J Clin Microbiol. 1981 Dec;14(6):681–683. doi: 10.1128/jcm.14.6.681-683.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts G. D., Horstmeier C., Hall M., Washington J. A., 2nd Recovery of yeast from vented blood culture bottles. J Clin Microbiol. 1975 Jul;2(1):18–20. doi: 10.1128/jcm.2.1.18-20.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]