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. 1983 Sep;18(3):526–528. doi: 10.1128/jcm.18.3.526-528.1983

Elimination of multiple reactions of the Phadebact Streptococcus coagglutination test.

D E Jones, K S Kanarek, J L Angel, D V Lim
PMCID: PMC270847  PMID: 6355146

Abstract

Strong multiple reactions often occur with the Phadebact Streptococcus test when the culture contains blood. These reactions interfere with the identification of the Lancefield groups of streptococci. Group B streptococci from the vagina of pregnant women are difficult to identify by slide coagglutination because of the frequent presence of blood on culture swabs. Elimination of these multiple reactions caused by blood would permit rapid identification of group B streptococci in pregnant women. Vaginal broth cultures were examined to determine the cause of multiple reactions with slide coagglutination and to eliminate them from the testing procedure. Of 245 maternal broth cultures, 135 (55%) yielded multiple reactions when tested by coagglutination. Such reactions were either eliminated or greatly diminished by heating the broth sample to 90 degrees C for 10 min. It was also found that globulins in the serum may be responsible for multiple reactions with blood. This heating protocol will permit vaginal broth cultures to be rapidly tested for group B streptococci by slide coagglutination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker C. J., Clark D. J., Barrett F. F. Selective broth medium for isolation of group B streptococci. Appl Microbiol. 1973 Dec;26(6):884–885. doi: 10.1128/am.26.6.884-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker C. J., Goroff D. K., Alpert S. L., Hayes C., McCormack W. M. Comparison of bacteriological methods for the isolation of group of B Streptococcus from vaginal cultures. J Clin Microbiol. 1976 Jul;4(1):46–48. doi: 10.1128/jcm.4.1.46-48.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benchetrit L. C., Fracalanzza S. E., Peregrino H., Camelo A. A., Sanches L. A. Carriage of Streptococcus agalactiae in women and neonates and distribution of serological types: a study in Brazil. J Clin Microbiol. 1982 May;15(5):787–790. doi: 10.1128/jcm.15.5.787-790.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Damask L. J., Montoya O., Axelrod J. L. Rapid slide agglutination test for Lancefield grouping of streptococci. Arch Pathol Lab Med. 1979 Aug;103(9):456–458. [PubMed] [Google Scholar]
  5. Fenton L. J., Harper M. H. Evaluation of colistin and nalidixic acid in Todd-Hewitt broth for selective isolation of group B streptocci. J Clin Microbiol. 1979 Feb;9(2):167–169. doi: 10.1128/jcm.9.2.167-169.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gray B. M., Pass M. A., Dillon H. C., Jr Laboratory and field evaluation of selective media for isolation of group B streptococci. J Clin Microbiol. 1979 Apr;9(4):466–470. doi: 10.1128/jcm.9.4.466-470.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hahn G., Nyberg I. Identification of streptococcal groups A,B,C, and G by slide co-agglutination of antibody-sensitized protein A-containing staphylococci. J Clin Microbiol. 1976 Jul;4(1):99–101. doi: 10.1128/jcm.4.1.99-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoogkamp-Korstanje J. A., Gerards L. J., Cats B. P. Maternal carriage and neonatal acquisition of group B streptococci. J Infect Dis. 1982 Jun;145(6):800–803. doi: 10.1093/infdis/145.6.800. [DOI] [PubMed] [Google Scholar]
  9. Iams J. D., O'Shaughnessy R. Antepartum versus intrapartum selective screening for maternal group B streptococcal colonization. Am J Obstet Gynecol. 1982 May 15;143(2):153–156. doi: 10.1016/0002-9378(82)90645-7. [DOI] [PubMed] [Google Scholar]
  10. Lim D. V., Smith R. D., Day S. Evaluation of an improved rapid coagglutination method for the serological grouping of beta-hemolytic streptococci. Can J Microbiol. 1979 Jan;25(1):40–43. doi: 10.1139/m79-006. [DOI] [PubMed] [Google Scholar]
  11. Rosner R. Laboratory evaluation of a rapid four-hour serological grouping of groups A,B,C, and G beta-streptococci by the Phadebact streptococcus test. J Clin Microbiol. 1977 Jul;6(1):23–26. doi: 10.1128/jcm.6.1.23-26.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stoner R. A. Bacitracin and coagglutination for grouping of beta-hemolytic streptococci. J Clin Microbiol. 1978 May;7(5):463–466. doi: 10.1128/jcm.7.5.463-466.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Summary of the workshop on perinatal infections due to group B Streptococcus. J Infect Dis. 1977 Jul;136(1):137–152. doi: 10.1093/infdis/136.1.137. [DOI] [PubMed] [Google Scholar]
  14. Szilagyi G., Mayer E., Eidelman A. I. Rapid isolation and identification of group B streptococci from selective broth medium by slide co-agglutination test. J Clin Microbiol. 1978 Oct;8(4):410–412. doi: 10.1128/jcm.8.4.410-412.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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