Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1983 Sep;18(3):535–538. doi: 10.1128/jcm.18.3.535-538.1983

Inactivation of hepatitis B virus by intermediate-to-high-level disinfectant chemicals.

W W Bond, M S Favero, N J Petersen, J W Ebert
PMCID: PMC270849  PMID: 6630443

Abstract

In five separate tests, hepatitis B virus in dried human plasma was exposed for 10 min at 20 degrees C to disinfectant chemicals having activity levels ranging from intermediate (e.g., 70% isopropyl alcohol) to high (e.g., 2% aqueous glutaraldehyde at pH 8.6). Five chimpanzees (one animal per disinfectant chemical) received treated material intravenously, and none showed signs of infection after post-inoculation periods of 9 months. Two animals were rechallenged with inoculum treated in the same manner, except that saline was used instead of a disinfectant chemical; both were infected within 4 weeks. Our results showed that hepatitis B virus was not as resistant to disinfectant chemicals as once thought and suggested that chemicals with similar activity levels (intermediate to high) might possibly be used on hepatitis B virus contamination with a margin of safety.

Full text

PDF
535

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bond W. W., Favero M. S., Petersen N. J., Gravelle C. R., Ebert J. W., Maynard J. E. Survival of hepatitis B virus after drying and storage for one week. Lancet. 1981 Mar 7;1(8219):550–551. doi: 10.1016/s0140-6736(81)92877-4. [DOI] [PubMed] [Google Scholar]
  2. Bond W. W., Moncada R. E. Viral hepatitis B infection risk in flexible fiberoptic endoscopy. Gastrointest Endosc. 1978 Aug;24(5):225–232. doi: 10.1016/s0016-5107(78)73517-0. [DOI] [PubMed] [Google Scholar]
  3. Bond W. W., Petersen N. J., Favero M. S. Viral hepatitis B: aspects of environmental control. Health Lab Sci. 1977 Oct;14(4):235–252. [PubMed] [Google Scholar]
  4. Bond W. W., Peterson N. J., Favero M. S., Ebert J. W., Maynard J. E. Transmission of type B viral hepatitis via eye inoculation of a chimpanzee. J Clin Microbiol. 1982 Mar;15(3):533–534. doi: 10.1128/jcm.15.3.533-534.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dankert J., Uitentuis J., Houwen B., Tegzess A. M., van der Hem G. K. Hepatitis B surface antigen in environmental samples from hemodialysis units. J Infect Dis. 1976 Aug;134(2):123–127. doi: 10.1093/infdis/134.2.123. [DOI] [PubMed] [Google Scholar]
  6. Favero M. S., Bond W. W., Petersen N. J., Berquist K. R., Maynard J. E. Detection methods for study of the stability of hepatitis B antigen on surfaces. J Infect Dis. 1974 Feb;129(2):210–212. doi: 10.1093/infdis/129.2.210. [DOI] [PubMed] [Google Scholar]
  7. Favero M. S., Maynard J. E., Petersen N. J., Boyer K. M., Bond W. W., Berquist K. R., Szmuness W. Letter: Hepatitis-B antigen on environmental surfaces. Lancet. 1973 Dec 22;2(7843):1455–1455. doi: 10.1016/s0140-6736(73)92860-2. [DOI] [PubMed] [Google Scholar]
  8. Francis D. P., Favero M. S., Maynard J. E. Transmission of hepatitis B virus. Semin Liver Dis. 1981 Feb;1(1):27–32. doi: 10.1055/s-2008-1063927. [DOI] [PubMed] [Google Scholar]
  9. Francis D. P., Maynard J. E. The transmission and outcome of hepatitis A, B, and non-A, non-B: a review. Epidemiol Rev. 1979;1:17–31. doi: 10.1093/oxfordjournals.epirev.a036208. [DOI] [PubMed] [Google Scholar]
  10. Kobayashi H., Takahashi Y., Tsuzuki M., Yoshihara N., Toyama H. Sterilization of hepatitis B surface antigen-contaminated materials. Med Instrum. 1978 May-Jun;12(3):171–173. [PubMed] [Google Scholar]
  11. Lauer J. L., VanDrunen N. A., Washburn J. W., Balfour H. H., Jr Transmission of hepatitis B virus in clinical laboratory areas. J Infect Dis. 1979 Oct;140(4):513–516. doi: 10.1093/infdis/140.4.513. [DOI] [PubMed] [Google Scholar]
  12. Moniz E., Feldman F., Newkirk M., Feinman S. V., Berris B. Removal of hepatitis B surface antigen from a contaminated applanation tonometer. Am J Ophthalmol. 1981 Apr;91(4):522–525. doi: 10.1016/0002-9394(81)90243-9. [DOI] [PubMed] [Google Scholar]
  13. Nath N., Fang C. T., Dodd R. Y. Inactivation of DNA-polymerase associated with hepatitis B virus. J Med Virol. 1982;10(2):131–140. doi: 10.1002/jmv.1890100207. [DOI] [PubMed] [Google Scholar]
  14. Petersen N. J., Barrett D. H., Bond W. W., Berquist K. R., Favero M. S., Bender T. R., Maynard J. E. Hepatitis B surface antigen in saliva, impetiginous lesions, and the environment in two remote Alaskan villages. Appl Environ Microbiol. 1976 Oct;32(4):572–574. doi: 10.1128/aem.32.4.572-574.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Seder R. H., Desai P. M., Keff R. S. Letter: Laboratory-acquired hepatitis B. Lancet. 1975 Dec 27;2(7948):1316–1316. doi: 10.1016/s0140-6736(75)90662-5. [DOI] [PubMed] [Google Scholar]
  16. Sehulster L. M., Hollinger F. B., Dreesman G. R., Melnick J. L. Immunological and biophysical alteration of hepatitis B virus antigens by sodium hypochlorite disinfection. Appl Environ Microbiol. 1981 Nov;42(5):762–767. doi: 10.1128/aem.42.5.762-767.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shikata T., Karasawa T., Abe K., Takahashi T., Mayumi M., Oda T. Incomplete inactivation of hepatitis B virus after heat treatment at 60 C for 10 hours. J Infect Dis. 1978 Aug;138(2):242–244. doi: 10.1093/infdis/138.2.242. [DOI] [PubMed] [Google Scholar]
  18. Stephan W., Berthold H., Prince A. M. Effect of combined treatment of serum containing hepatitis B virus with beta-propiolactone and ultraviolet irradiation. Vox Sang. 1981 Sep;41(3):134–138. doi: 10.1111/j.1423-0410.1981.tb01026.x. [DOI] [PubMed] [Google Scholar]
  19. Tabor E., Murano G., Snoy P., Gerety R. J. Inactivation of hepatitis B virus by heat in antithrombin III stabilized with citrate. Thromb Res. 1981 Apr 1;22(1-2):233–238. doi: 10.1016/0049-3848(81)90326-1. [DOI] [PubMed] [Google Scholar]
  20. Thraenhart O., Kuwert E. K. Hepatitis-Viruzidie chemischer Desinfektionsmittel. MMW Munch Med Wochenschr. 1980 Mar 7;122(10):357–359. [PubMed] [Google Scholar]
  21. Zachoval R., Frösner G., Deinhardt F., Höss D. Persistence of hepatitis B virus antigens in dried blood. Lancet. 1981 Apr 4;1(8223):778–778. doi: 10.1016/s0140-6736(81)92649-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES