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. 1983 Sep;18(3):558–560. doi: 10.1128/jcm.18.3.558-560.1983

Rapid identification of pregnant women heavily colonized with group B streptococci.

D E Jones, E M Friedl, K S Kanarek, J K Williams, D V Lim
PMCID: PMC270854  PMID: 6355149

Abstract

Pregnant women admitted to Tampa General Hospital, Tampa, Fla., were cultured for group B streptococci (GBS). Culture swabs were placed into enriched, selective Todd-Hewitt medium and were quantitated for GBS. The broth cultures were tested by slide coagglutination before incubation and after 5 and 20 h of incubation. Fifty-four (27%) of the 201 maternity patients cultured were positive for GBS and were identified as such by slide coagglutination. A strong correlation was found between the magnitudes of colonization and the times required to identify the broth cultures as GBS positive. Cultures from mothers heavily colonized (mean concentrations of 3 X 10(4) GBS per culture swab or greater) were identified after 5 h or less of incubation. Mothers lightly colonized with GBS (mean concentrations of 2 X 10(2) GBS per culture swab) were identified only after their broth cultures had been incubated for 20 h.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ancona R. J., Ferrieri P., Williams P. P. Maternal factors that enhance the acquisition of group-B streptococci by newborn infants. J Med Microbiol. 1980 May;13(2):273–280. doi: 10.1099/00222615-13-2-273. [DOI] [PubMed] [Google Scholar]
  2. Anthony B. F. Carriage of group B streptococci during pregnancy: a puzzler. J Infect Dis. 1982 Jun;145(6):789–793. doi: 10.1093/infdis/145.6.789. [DOI] [PubMed] [Google Scholar]
  3. Ayres D. C., Lim C. K. Modification of the pendant ring of podophyllotoxin. Cancer Chemother Pharmacol. 1982;7(2-3):99–101. doi: 10.1007/BF00254529. [DOI] [PubMed] [Google Scholar]
  4. Baker C. J., Goroff D. K., Alpert S. L., Hayes C., McCormack W. M. Comparison of bacteriological methods for the isolation of group of B Streptococcus from vaginal cultures. J Clin Microbiol. 1976 Jul;4(1):46–48. doi: 10.1128/jcm.4.1.46-48.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benchetrit L. C., Fracalanzza S. E., Peregrino H., Camelo A. A., Sanches L. A. Carriage of Streptococcus agalactiae in women and neonates and distribution of serological types: a study in Brazil. J Clin Microbiol. 1982 May;15(5):787–790. doi: 10.1128/jcm.15.5.787-790.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fenton L. J., Harper M. H. Evaluation of colistin and nalidixic acid in Todd-Hewitt broth for selective isolation of group B streptocci. J Clin Microbiol. 1979 Feb;9(2):167–169. doi: 10.1128/jcm.9.2.167-169.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hahn G., Nyberg I. Identification of streptococcal groups A,B,C, and G by slide co-agglutination of antibody-sensitized protein A-containing staphylococci. J Clin Microbiol. 1976 Jul;4(1):99–101. doi: 10.1128/jcm.4.1.99-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoogkamp-Korstanje J. A., Gerards L. J., Cats B. P. Maternal carriage and neonatal acquisition of group B streptococci. J Infect Dis. 1982 Jun;145(6):800–803. doi: 10.1093/infdis/145.6.800. [DOI] [PubMed] [Google Scholar]
  9. Iams J. D., O'Shaughnessy R. Antepartum versus intrapartum selective screening for maternal group B streptococcal colonization. Am J Obstet Gynecol. 1982 May 15;143(2):153–156. doi: 10.1016/0002-9378(82)90645-7. [DOI] [PubMed] [Google Scholar]
  10. Lim D. V., Smith R. D., Day S. Evaluation of an improved rapid coagglutination method for the serological grouping of beta-hemolytic streptococci. Can J Microbiol. 1979 Jan;25(1):40–43. doi: 10.1139/m79-006. [DOI] [PubMed] [Google Scholar]
  11. Manos J. P. Group B streptococcal infection in the neonate. Ann Clin Lab Sci. 1982 May-Jun;12(3):239–243. [PubMed] [Google Scholar]
  12. Ryan M. E., Barrett F. F. Rapid detection of group B streptococcal colonization by a direct immunofluorescent antibody technique. J Pediatr. 1982 Dec;101(6):993–995. doi: 10.1016/s0022-3476(82)80028-0. [DOI] [PubMed] [Google Scholar]
  13. Summary of the workshop on perinatal infections due to group B Streptococcus. J Infect Dis. 1977 Jul;136(1):137–152. doi: 10.1093/infdis/136.1.137. [DOI] [PubMed] [Google Scholar]
  14. Szilagyi G., Mayer E., Eidelman A. I. Rapid isolation and identification of group B streptococci from selective broth medium by slide co-agglutination test. J Clin Microbiol. 1978 Oct;8(4):410–412. doi: 10.1128/jcm.8.4.410-412.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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