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. 1983 Sep;18(3):668–673. doi: 10.1128/jcm.18.3.668-673.1983

Development of fluorescent-antibody reagents for demonstration of Pseudallescheria boydii in tissues.

J A Jackson, W Kaplan, L Kaufman, P Standard
PMCID: PMC270872  PMID: 6195179

Abstract

We prepared fluorescent-antibody reagents to detect and identify Pseudallescheria boydii in tissue. Antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of P. boydii were produced in rabbits. Antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain P. boydii in tissues. The conjugates were first tested with cultures of 25 P. boydii isolates and 26 heterologous fungi that are morphologically similar to P. boydii in tissues. Labeled antibody to the soluble antigens reacted only weakly with cultures of P. boydii. Labeled antibody to particulate antigens brightly stained all P. boydii isolates and cross-reacted with Aspergillus flavus, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus terreus, Fusarium oxysporum, Fusarium solani, and a Scopulariopsis sp. The cross-reacting antibodies were removed by adsorption with A. fumigatus and F. oxysporum. The adsorbed conjugates stained P. boydii in Formalin-fixed tissues from experimentally infected mice and in Formalin-fixed tissues from humans and animals with natural infections. They did not stain the heterologous fungi in tissues.

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Selected References

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  1. AJELLO L. The isolation of Aliescheria boydii shear, an etiologic agent of mycetomas, from soil. Am J Trop Med Hyg. 1952 Mar;1(2):227–238. doi: 10.4269/ajtmh.1952.1.227. [DOI] [PubMed] [Google Scholar]
  2. Baxter M., Murray I. G., Taylor J. J. A case of mycetoma with serological diagnosis of Allescheria boydii. Sabouraudia. 1966 Oct;5(2):138–140. doi: 10.1080/00362176785190261. [DOI] [PubMed] [Google Scholar]
  3. Bryan C. S., DiSalvo A. F., Kaufman L., Kaplan W., Brill A. H., Abbott D. C. Petriellidium boydii infection of the sphenoid sinus. Am J Clin Pathol. 1980 Dec;74(6):846–851. doi: 10.1093/ajcp/74.6.846. [DOI] [PubMed] [Google Scholar]
  4. COOKE W. B., KABLER P. Isolation of potentially pathogenic fungi from polluted water and sewage. Public Health Rep. 1955 Jul;70(7):689–694. [PMC free article] [PubMed] [Google Scholar]
  5. Ernest J. T., Rippon J. W. Keratitis due to Allescheria boydii (Monosporium apiospermum). Am J Ophthalmol. 1966 Dec;62(6):1202–1204. doi: 10.1016/0002-9394(66)92576-1. [DOI] [PubMed] [Google Scholar]
  6. Forno L. S., Billingham M. E. Allescheria boydii infection of the brain. J Pathol. 1972 Mar;106(3):195–198. doi: 10.1002/path.1711060307. [DOI] [PubMed] [Google Scholar]
  7. GREEN W. O., Jr, ADAMS T. E. MYCETOMA IN THE UNITED STATES; A REVIEW AND REPORT OF SEVEN ADDITIONAL CASES. Am J Clin Pathol. 1964 Jul;42:75–91. doi: 10.1093/ajcp/42.1.75. [DOI] [PubMed] [Google Scholar]
  8. Gluckman S. J., Ries K., Abrutyn E. Allescheria (Petriellidium) boydii sinusitis in a compromised host. J Clin Microbiol. 1977 Apr;5(4):481–484. doi: 10.1128/jcm.5.4.481-484.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jang S. S., Popp J. A. Eumycotic mycetoma in a dog caused by Allescheria boydii. J Am Vet Med Assoc. 1970 Oct 15;157(8):1071–1076. [PubMed] [Google Scholar]
  10. KAPLAN W., IVENS M. S. Fluorescent antibody staining of Sporotrichum schenckii in cultures and clinical materials. J Invest Dermatol. 1960 Sep;35:151–159. doi: 10.1038/jid.1960.99. [DOI] [PubMed] [Google Scholar]
  11. Kaplan W., Kraft D. E. Demonstration of pathogenic fungi in formalin-fixed tissues by immunofluorescence. Am J Clin Pathol. 1969 Oct;52(4):420–432. doi: 10.1093/ajcp/52.4.420. [DOI] [PubMed] [Google Scholar]
  12. Knudtson W. U., Wohlgemuth K., Kirkbride C. A., Robl M. G., Vorhies M. W., McAdaragh J. P. Mycologic, serologic and histologic findings in bovine abortion associated with Allescheria boydii. Sabouraudia. 1974 Mar;12(1):81–86. doi: 10.1080/00362177485380111. [DOI] [PubMed] [Google Scholar]
  13. Kurtz H. J., Finco D. R., Perman V. Maduromycosis (Allescheria boydii) in a dog. J Am Vet Med Assoc. 1970 Oct 1;157(7):917–921. [PubMed] [Google Scholar]
  14. Lupan D. M., Cazin J., Jr Serological diagnosis of petriellidiosis (allescheriosis). II. Indirect (passive) hemagglutination assay for antibody to polysaccharide antigens of Pertriellidium (Allescheria) boydii and Monosporium apiospermum. Mycopathologia. 1977 Dec 16;62(2):87–95. doi: 10.1007/BF01259397. [DOI] [PubMed] [Google Scholar]
  15. Lutwick L. I., Galgiani J. N., Johnson R. H., Stevens D. A. Visceral fungal infections due to Petriellidium boydii (allescheria boydii). In vitro drug sensitivity studies. Am J Med. 1976 Nov;61(5):632–640. doi: 10.1016/0002-9343(76)90141-8. [DOI] [PubMed] [Google Scholar]
  16. MAHAFFEY L. W., ROSSDALE P. D. AN ABORTION DUE TO ALLESCHERIA BOYDII AND GENERAL OBSERVATIONS CONCERNING MYCOTIC ABORTIONS OF MARES. Vet Rec. 1965 May 8;77:541–545. [PubMed] [Google Scholar]
  17. MAHGOUB E. S. THE VALUE OF GEL DIFFUSIONS IN THE DIAGNOSIS OF MYCETOMA. Trans R Soc Trop Med Hyg. 1964 Nov;58:560–563. doi: 10.1016/0035-9203(64)90114-2. [DOI] [PubMed] [Google Scholar]
  18. MEYER E., HERROLD R. D. Allescheria boydii isolated from a patient with chronic prostatitis. Am J Clin Pathol. 1961 Feb;35:155–159. doi: 10.1093/ajcp/35.2.155. [DOI] [PubMed] [Google Scholar]
  19. McGinnis M. R. Recent taxonomic developments and changes in medical mycology. Annu Rev Microbiol. 1980;34:109–135. doi: 10.1146/annurev.mi.34.100180.000545. [DOI] [PubMed] [Google Scholar]
  20. Morace G., Polonelli L. Exoantigen test for identification of Petriellidium boydii cultures. J Clin Microbiol. 1981 Sep;14(3):237–240. doi: 10.1128/jcm.14.3.237-240.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Murray I. G., Mahgoub E. S. Further studies on the diagnosis of mycetoma by double diffusion in agar. Sabouraudia. 1968 Feb;6(2):106–110. doi: 10.1080/00362176885190201. [DOI] [PubMed] [Google Scholar]
  22. Reid M. M., Frock I. W., Jeffrey D. R., Kaiser G. E. Successful treatment of a maduromycotic fungal infection of the equine uterus with amphotericin B. Vet Med Small Anim Clin. 1977 Jul;72(7):1194–1196. [PubMed] [Google Scholar]
  23. Reid M. M., Jeffrey D. R., Kaiser G. E. A rare case of maduromycosis of the equine uterus. Vet Med Small Anim Clin. 1976 Jul;71(7):947–949. [PubMed] [Google Scholar]
  24. SEELIGER H. A serologic study of Hyphomycetes causing mycetoma in man. J Invest Dermatol. 1956 Jan;26(1):81–93. doi: 10.1038/jid.1956.8. [DOI] [PubMed] [Google Scholar]
  25. Winston D. J., Jordan M. C., Rhodes J. Allescheria boydii infections in the immunosuppressed host. Am J Med. 1977 Nov;63(5):830–835. doi: 10.1016/0002-9343(77)90170-x. [DOI] [PubMed] [Google Scholar]

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