Abstract
The detection of thermonuclease activity in 86 blood culture samples containing gram-positive cocci showed 100% correlation with the subsequent identification of the isolate as Staphylococcus aureus by the coagulase test. No positive thermonuclease results were found with 66 samples containing coagulase-negative staphylococci and 56 samples containing other gram-positive organisms. The thermonuclease test provides a rapid, reliable method to identify S. aureus in blood cultures.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Agger W. A., Maki D. G. Efficacy of direct Gram stain in differentiating staphylococci from streptococci in blood cultures positive for gram-positive cocci. J Clin Microbiol. 1978 Feb;7(2):111–113. doi: 10.1128/jcm.7.2.111-113.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barry A. L., Lachica R. V., Atchison F. W. Identification of Staphylococcus aureus by simultaneous use of tube coagulase and thermonuclease tests. Appl Microbiol. 1973 Mar;25(3):496–497. doi: 10.1128/am.25.3.496-497.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doern G. V., Robbie L. I. Direct identification of Staphylococcus aureus in blood culture fluid with a commercial latex agglutination test. J Clin Microbiol. 1982 Dec;16(6):1048–1051. doi: 10.1128/jcm.16.6.1048-1051.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Erickson A., Deibel R. H. Production and heat stability of staphylococcal nuclease. Appl Microbiol. 1973 Mar;25(3):332–336. doi: 10.1128/am.25.3.332-336.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gramoli J. L., Wilkinson B. J. Characterization and identification of coagulase-negative, heat-stable deoxyribonuclease-positive staphylococci. J Gen Microbiol. 1978 Apr;105(2):275–285. doi: 10.1099/00221287-105-2-275. [DOI] [PubMed] [Google Scholar]
- Knight R. G., Shlaes D. M. Rapid identification of Staphylococcus aureus and Streptococcus pneumoniae from blood cultures. J Clin Microbiol. 1983 Jan;17(1):97–99. doi: 10.1128/jcm.17.1.97-99.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koupal A., Deibel R. H. Rapid qualitative method for detecting staphylococcal nuclease in foods. Appl Environ Microbiol. 1978 Jun;35(6):1193–1197. doi: 10.1128/aem.35.6.1193-1197.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lachica R. V., Genigeorgis C., Hoeprich P. D. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl Microbiol. 1971 Apr;21(4):585–587. doi: 10.1128/am.21.4.585-587.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lachica R. V., Hoeprich P. D., Genigeorgis C. Metachromatic agar-diffusion microslide technique for detecting staphylococcal nuclease in foods. Appl Microbiol. 1972 Jan;23(1):168–169. doi: 10.1128/am.23.1.168-169.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacGregor R. R., Beaty H. N. Evaluation of positive blood cultures. Guidelines for early differentiation of contaminated from valid positive cultures. Arch Intern Med. 1972 Jul;130(1):84–87. [PubMed] [Google Scholar]
- Marsik F. J., Brake S. Species identification and susceptibility to 17 antibiotics of coagulase-negative staphylococci isolated from clinical specimens. J Clin Microbiol. 1982 Apr;15(4):640–645. doi: 10.1128/jcm.15.4.640-645.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Menzies R. E. Comparison of coagulase, deoxyribonuclease (DNase), and heat-stable nuclease tests for identification of Staphylococcus aureus. J Clin Pathol. 1977 Jul;30(7):606–608. doi: 10.1136/jcp.30.7.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Park C. E., El Derea H. B., Rayman M. K. Evaluation of staphylococcal thermonuclease (TNase) assay as a means of screening foods for growth of staphylococci and possible enterotoxin production. Can J Microbiol. 1978 Oct;24(10):1135–1139. doi: 10.1139/m78-185. [DOI] [PubMed] [Google Scholar]
- Park C. E., de Melo Serrano A., Landgraf M., Huang J. C., Stankiewicz Z., Rayman M. K. A survey of microorganisms for thermonuclease production. Can J Microbiol. 1980 Apr;26(4):532–538. doi: 10.1139/m80-089. [DOI] [PubMed] [Google Scholar]
- Rayman M. K., Park C. E., Philpott J., Todd E. C. Reassessment of the coagulase and thermostable nuclease tests as means of identifying Staphylococcus aureus. Appl Microbiol. 1975 Apr;29(4):451–454. doi: 10.1128/am.29.4.451-454.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Severance P. J., Kauffman C. A., Sheagren J. N. Rapid identification of Staphylococcus aureus by using lysostaphin sensitivity. J Clin Microbiol. 1980 Jun;11(6):724–727. doi: 10.1128/jcm.11.6.724-727.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sewell C. M., Clarridge J. E., Young E. J., Guthrie R. K. Clinical significance of coagulase-negative staphylococci. J Clin Microbiol. 1982 Aug;16(2):236–239. doi: 10.1128/jcm.16.2.236-239.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shanholtzer C. J., Peterson L. R. Clinical laboratory evaluation of the thermonuclease test. Am J Clin Pathol. 1982 May;77(5):587–591. doi: 10.1093/ajcp/77.5.587. [DOI] [PubMed] [Google Scholar]
- Wasilauskas B. L., Ellner P. D. Presumptive identification of bacteria from blood cultures in four hours. J Infect Dis. 1971 Nov;124(5):499–504. doi: 10.1093/infdis/124.5.499. [DOI] [PubMed] [Google Scholar]
- Zarzour J. Y., Belle E. A. Evaluation of three test procedures for identification of Staphylococcus aureus from clinical sources. J Clin Microbiol. 1978 Feb;7(2):133–136. doi: 10.1128/jcm.7.2.133-136.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]