Fig. 6.

Disc1 repressed foxd3 and sox10 expression. (A) Injection of 200 pg of gfp mRNA into one cell of a two- to four-cell-stage embryo resulted in unilateral expression at 5s. Dotted line outlines the edge of the embryo. (B-E) Asymmetrical overexpression of the human ortholog of disc1 resulted in a unilateral loss of foxd3 (B,C) and sox10 (D,E) expression (arrows). Expression of myod1 was normal. Dorsal views with anterior to the left. (F-I) Overexpression of Foxd3 resulted in an expansion of sox10 expression (arrows in H,I) similar to that seen in Disc1 morphants. F and G are lateral views, H and I, dorsal views, both with anterior to the left.