Abstract
Hybridoma-producing monoclonal antibodies against Legionella pneumophila were produced by the fusion of nonsecreting mouse myeloma cells (NS-1) with splenocytes of BALB/c mice immunized by heat-killed L. pneumophila of serogroup I. Of 96 wells, 85 produced clones, of which 28 were positive as measured by an enzyme-linked immunosorbent assay technique. Ten of the hybridoma supernatants, remaining positive after 2 months of culture, were tested against the other Legionella serogroups and the atypical strains. None showed significant cross-reaction. Six of the positive clones were subcloned by limiting dilution, and two subclones were put into ascites in BALB/c mice. The monoclonal antibody obtained from the II-6-18 subclone was of the gamma-3 isotype. In this report, we describe the conditions for the use of this monoclonal antibody as a diagnostic tool for the detection of serogroup I L. pneumophila.
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- Edelstein P. H., McKinney R. M., Meyer R. D., Edelstein M. A., Krause C. J., Finegold S. M. Immunologic diagnosis of Legionnaires' disease: cross-reactions with anaerobic and microaerophilic organisms and infections caused by them. J Infect Dis. 1980 May;141(5):652–655. doi: 10.1093/infdis/141.5.652. [DOI] [PubMed] [Google Scholar]
- Eickhoff T. C. Epidemiology of Legionnaires' disease. Ann Intern Med. 1979 Apr;90(4):499–502. doi: 10.7326/0003-4819-90-4-499. [DOI] [PubMed] [Google Scholar]
- Fallon R. J., Abraham W. H. Scottish experience with the serologic diagnosis of Legionnaires' disease. Ann Intern Med. 1979 Apr;90(4):684–686. doi: 10.7326/0003-4819-90-4-684. [DOI] [PubMed] [Google Scholar]
- Farshy C. E., Klein G. C., Feeley J. C. Detection of antibodies to legionnaires disease organism by microagglutination and micro-enzyme-linked immunosorbent assay tests. J Clin Microbiol. 1978 Apr;7(4):327–331. doi: 10.1128/jcm.7.4.327-331.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gump D. W., Frank R. O., Winn W. C., Jr, Foster R. S., Jr, Broome C. V., Cherry W. B. Legionnaires' disease in patients with associated serious disease. Ann Intern Med. 1979 Apr;90(4):538–542. doi: 10.7326/0003-4819-90-4-538. [DOI] [PubMed] [Google Scholar]
- Johnson W., Pesanti E., Elliott J. Serospecificity and opsonic activity of antisera to Legionella pneumophila. Infect Immun. 1979 Nov;26(2):698–704. doi: 10.1128/iai.26.2.698-704.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Macfarlane J. T., Finch R. G., Ward M. J., Macrae A. D. Hospital study of adult community-acquired pneumonia. Lancet. 1982 Jul 31;2(8292):255–258. doi: 10.1016/s0140-6736(82)90334-8. [DOI] [PubMed] [Google Scholar]
- McKinney R. M., Thacker L., Harris P. P., Lewallen K. R., Hebert G. A., Edelstein P. H., Thomason B. M. Four serogroups of Legionnaires' disease bacteria defined by direct immunofluorescence. Ann Intern Med. 1979 Apr;90(4):621–624. doi: 10.7326/0003-4819-90-4-621. [DOI] [PubMed] [Google Scholar]
- Swartz M. N. Clinical aspects of Legionnaires' disease. Ann Intern Med. 1979 Apr;90(4):492–495. doi: 10.7326/0003-4819-90-4-492. [DOI] [PubMed] [Google Scholar]
- Tilton R. C. Legionnaires' disease antigen detected by enzyme-linked immunosorbent assay. Ann Intern Med. 1979 Apr;90(4):697–698. doi: 10.7326/0003-4819-90-4-697. [DOI] [PubMed] [Google Scholar]