Abstract
Staining 2,205 macroscopically negative blood cultures with acridine orange after 6 to 17 h of inoculation and incubation was as sensitive as an early subculture in detecting positive blood cultures. Of the 179 positive blood cultures, 30 (16.8%) were detected by acridine orange alone, 19 (10.6%) were detected by early subculture alone, 84 (46.9%) were detected by both techniques, and 46 (25.7%) were not detected by either method. The latter group includes cultures that became positive after 48 h of incubation. Acridine orange staining of smears prepared from macroscopically negative blood cultures after 6 to 17 h is a rapid, reliable method to detect positive blood cultures.
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Selected References
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- Kronvall G., Myhre E. Differential staining of bacteria in clinical specimens using acridine orange buffered at low pH. Acta Pathol Microbiol Scand B. 1977 Aug;85(4):249–254. doi: 10.1111/j.1699-0463.1977.tb01970.x. [DOI] [PubMed] [Google Scholar]
- McCarthy L. R., Senne J. E. Evaluation of acridine orange stain for detection of microorganisms in blood cultures. J Clin Microbiol. 1980 Mar;11(3):281–285. doi: 10.1128/jcm.11.3.281-285.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mirrett S., Lauer B. A., Miller G. A., Reller L. B. Comparison of acridine orange, methylene blue, and Gram stains for blood cultures. J Clin Microbiol. 1982 Apr;15(4):562–566. doi: 10.1128/jcm.15.4.562-566.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sliva H. S., Washington J. A., 2nd Optimal time for routine early subculture of blood cultures. J Clin Microbiol. 1980 Sep;12(3):445–446. doi: 10.1128/jcm.12.3.445-446.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]