Abstract
Accuracy, precision, and clinical laboratory utility of the TDX (Abbott Laboratories), Auto-ICS (Beckman Instruments, Inc.), COBAS-Bio (Roche Analytical Instruments, Inc.) with reagent kits (Syva), and EMIT (Syva) for gentamicin and tobramycin serum assay were assessed. TDX, COBAS-Bio, and EMIT analytical systems showed a proportional bias of less than 10% for recovery studies and a coefficient of variation less than 5% for within-run precision. The results of the recovery studies with the Auto-ICS showed a proportional bias of 25% with gentamicin and 16% with tobramycin. The within-run precision expressed as the coefficient of variation for the Auto-ICS was 6.7% for gentamicin and 8.6% for tobramycin. In comparisons involving gentamicin- and tobramycin-containing patient samples, the results with the TDX analytical system showed the best agreement with the COBAS-Bio. For the determination of these two antibiotics, the TDX analytical system provided the best overall accuracy and precision.
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Selected References
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- Cornbleet P. J., Gochman N. Incorrect least-squares regression coefficients in method-comparison analysis. Clin Chem. 1979 Mar;25(3):432–438. [PubMed] [Google Scholar]
- Lantz C. H., Lawrie D. J., Witebsky F. G., MacLowry J. D. Evaluation of serum gentamicin assay procedures for a clinical microbiology laboratory. J Clin Microbiol. 1980 Oct;12(4):583–589. doi: 10.1128/jcm.12.4.583-589.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ngui-Yen J. H., Hofmann T., Wigmore M., Smith J. A. Comparative evaluation of three methods for measuring gentamicin and tobramycin in serum. Antimicrob Agents Chemother. 1981 Dec;20(6):821–825. doi: 10.1128/aac.20.6.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Selepak S. T., Witebsky F. G., Robertson E. A., MacLowry J. D. Evaluation of five gentamicin assay procedures for clinical microbiology laboratories. J Clin Microbiol. 1981 Apr;13(4):742–749. doi: 10.1128/jcm.13.4.742-749.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens P., Young L. S., Hewitt W. L. Radioimmunoassay, acetylating radio-enzymatic assay, and microbioassay of gentamicin: a comparative study. J Lab Clin Med. 1975 Aug;86(2):349–359. [PubMed] [Google Scholar]