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. 1983 Oct;18(4):978–980. doi: 10.1128/jcm.18.4.978-980.1983

Serotyping of Streptococcus pneumoniae strains by coagglutination and counterimmunoelectrophoresis.

B Trollfors, L Burman, E Dannetun, J Llompart, R Norrby
PMCID: PMC270944  PMID: 6630474

Abstract

A total of 217 Streptococcus pneumoniae strains were serotyped by coagglutination (COA) and counterimmunoelectrophoresis (CIE). With all strains tested, there was full agreement between results obtained by COA and CIE, except for strains belonging to serotypes 7, 14, 33, and 37, which could not be typed by CIE. These strains were serotyped by passive immunodiffusion, results of which were in full agreement with those obtained by COA. Besides having the advantage of identifying strains belonging to all serotypes, COA was also more rapid and economical than CIE.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austrian R. The quellung reaction, a neglected microbiologic technique. Mt Sinai J Med. 1976 Nov-Dec;43(6):699–709. [PubMed] [Google Scholar]
  2. Coonrod J. D., Rytel M. W. Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. I. Methodology and immunologic properties of pneumococcal antigens. J Lab Clin Med. 1973 May;81(5):770–777. [PubMed] [Google Scholar]
  3. Henrichsen J., Berntsson E., Kaijser B. Comparison of counterimmunoelectrophoresis and the capsular reaction test for typing of pneumococci. J Clin Microbiol. 1980 Jun;11(6):589–592. doi: 10.1128/jcm.11.6.589-592.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kronvall G. A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. J Med Microbiol. 1973 May;6(2):187–190. doi: 10.1099/00222615-6-2-187. [DOI] [PubMed] [Google Scholar]
  5. Szu S. C., Oravec L. S. Detection of "neutral" type 7F and type 14 pneumococcal capsular polysaccharides by immunoelectrophoresis. J Clin Microbiol. 1982 Jun;15(6):1172–1175. doi: 10.1128/jcm.15.6.1172-1175.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. WADSWORTH C. A slide microtechnique for the analysis of immune precipitates in gel. Int Arch Allergy Appl Immunol. 1957;10(6):355–360. doi: 10.1159/000228394. [DOI] [PubMed] [Google Scholar]

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