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. 1984 Jan;19(1):21–25. doi: 10.1128/jcm.19.1.21-25.1984

Enzyme-linked immunosorbent assay compared with neutralization tests for evaluation of live mumps vaccines.

H Sakata, M Hishiyama, A Sugiura
PMCID: PMC270970  PMID: 6361060

Abstract

Mumps-specific antibody levels before and after vaccination with live mumps vaccines were determined by enzyme-linked immunosorbent assay (ELISA) and neutralization tests. A correlation was found between neutralization titers and optical density in ELISA. However, postvaccination sera from some vaccinees who failed to seroconvert by neutralization contained significant levels of mumps-specific antibody detectable by ELISA. In some of these serum specimens, the antibody directed to the F polypeptide of mumps virus was predominant. Most sera positive in ELISA neutralized mumps virus upon the addition of fresh guinea pig serum to the virus-serum mixture.

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Selected References

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  1. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  2. Choppin P. W., Scheid A. The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Rev Infect Dis. 1980 Jan-Feb;2(1):40–61. doi: 10.1093/clinids/2.1.40. [DOI] [PubMed] [Google Scholar]
  3. Ennis F. A. Immunity to mumps in an institutional epidemic. Correlation of insusceptibility to mumps with serum plaque neutralizing and hemagglutination-inhibiting antibodies. J Infect Dis. 1969 Jun;119(6):654–657. doi: 10.1093/infdis/119.6.654. [DOI] [PubMed] [Google Scholar]
  4. Herrler G., Compans R. W. Synthesis of mumps virus polypeptides in infected Vero cells. Virology. 1982 Jun;119(2):430–438. doi: 10.1016/0042-6822(82)90102-7. [DOI] [PubMed] [Google Scholar]
  5. Hilleman M. R., Weibel R. E., Buynak E. B., Stokes J., Jr, Whitman J. E., Jr Live attenuated mumps-virus vaccine. IV. Protective efficacy as measured in a field evaluation. N Engl J Med. 1967 Feb 2;276(5):252–258. doi: 10.1056/NEJM196702022760502. [DOI] [PubMed] [Google Scholar]
  6. Ho-Terry L., Cohen A. Rubella virion polypeptides: characterization by polyacrylamide gel electrophoresis, isoelectric focusing and peptide mapping. Arch Virol. 1982;72(1-2):47–54. doi: 10.1007/BF01314449. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Lamb R. A., Etkind P. R., Choppin P. W. Evidence for a ninth influenza viral polypeptide. Virology. 1978 Nov;91(1):60–78. doi: 10.1016/0042-6822(78)90355-0. [DOI] [PubMed] [Google Scholar]
  9. Leinikki P. O., Shekarchi I., Tzan N., Madden D. L., Sever J. L. Evaluation of enzyme-linked immunosorbent assay (ELISA) for mumps virus antibodies. Proc Soc Exp Biol Med. 1979 Mar;160(3):363–367. doi: 10.3181/00379727-160-40451. [DOI] [PubMed] [Google Scholar]
  10. McCarthy M., Johnson R. T. A comparison of the structural polypeptides of five strains of mumps virus. J Gen Virol. 1980 Jan;46(1):15–27. doi: 10.1099/0022-1317-46-1-15. [DOI] [PubMed] [Google Scholar]
  11. Merz D. C., Scheid A., Choppin P. W. Immunological studies of the functions of paramyxovirus glycoproteins. Virology. 1981 Feb;109(1):94–105. doi: 10.1016/0042-6822(81)90474-8. [DOI] [PubMed] [Google Scholar]
  12. Merz D. C., Scheid A., Choppin P. W. Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection. J Exp Med. 1980 Feb 1;151(2):275–288. doi: 10.1084/jem.151.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meurman O., Hänninen P., Krishna R. V., Ziegler T. Determination of IgG- and IgM-class antibodies to mumps virus by solid-phase enzyme immunoassay. J Virol Methods. 1982 May;4(4-5):249–256. doi: 10.1016/0166-0934(82)90071-4. [DOI] [PubMed] [Google Scholar]
  14. Naruse H., Nagai Y., Yoshida T., Hamaguchi M., Matsumoto T., Isomura S., Suzuki S. The polypeptides of mumps virus and their synthesis in infected chick embryo cells. Virology. 1981 Jul 15;112(1):119–130. doi: 10.1016/0042-6822(81)90618-8. [DOI] [PubMed] [Google Scholar]
  15. Orvell C. Identification of paramyxovirus-specific haemolysis-inhibiting antibodies separate from haemagglutinating-inhibiting and neuraminidase-inhibiting antibodies. 2. NDV and mumps virus haemolysis-inhibiting antibodies. Acta Pathol Microbiol Scand B. 1976 Dec;84B(6):451–457. [PubMed] [Google Scholar]
  16. Orvell C. Immunological properties of purified mumps virus glycoproteins. J Gen Virol. 1978 Dec;41(3):517–526. doi: 10.1099/0022-1317-41-3-517. [DOI] [PubMed] [Google Scholar]
  17. Orvell C. Structural polypeptides of mumps virus. J Gen Virol. 1978 Dec;41(3):527–539. doi: 10.1099/0022-1317-41-3-527. [DOI] [PubMed] [Google Scholar]
  18. Popow-Kraupp T. Enzyme-linked immunosorbent assay (ELISA) for mumps virus antibodies. J Med Virol. 1981;8(2):79–88. doi: 10.1002/jmv.1890080202. [DOI] [PubMed] [Google Scholar]
  19. Popow-Kraupp T., Kunz C., Hofmann H. Antibody response after application of a new live attenuated mumps vaccine (Pariorix) measured by enzyme-linked immunosorbent assay (ELISA). J Med Virol. 1982;10(2):119–129. doi: 10.1002/jmv.1890100206. [DOI] [PubMed] [Google Scholar]
  20. Ukkonen P., Granström M. L., Penttinen K. Mumps-specific immunoglobulin M and G antibodies in natural mumps infection as measured by enzyme-linked immunosorbent assay. J Med Virol. 1981;8(2):131–142. doi: 10.1002/jmv.1890080207. [DOI] [PubMed] [Google Scholar]
  21. Ukkonen P., Väisänen O., Penttinen K. Enzyme-linked immunosorbent assay for mumps and parainfluenza type 1 immunoglobulin G and immunoglobulin M antibodies. J Clin Microbiol. 1980 Apr;11(4):319–323. doi: 10.1128/jcm.11.4.319-323.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yamanishi K., Takahashi M., Ueda S., Minekawa Y., Ogino T. Studies on live mumps virus vaccine. V. Development of a new mumps vaccine "AM 9" by plaque cloning. Biken J. 1973 Dec;16(4):161–166. [PubMed] [Google Scholar]

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