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. 1984 Jan;19(1):68–72. doi: 10.1128/jcm.19.1.68-72.1984

Comparison of the API Staph-Ident and DMS Staph-Trac systems with conventional methods used for the identification of coagulase-negative staphylococci.

O Giger, C C Charilaou, K R Cundy
PMCID: PMC270981  PMID: 6361063

Abstract

Two commercial kit systems, the API Staph-Ident system (Analytab Products, Inc., Plainview, N.Y.) and the DMS Staph-Trac system (DMS Laboratories, Inc., Flemington, N.J.), were compared with conventional methods for the identification of nine species of coagulase-negative staphylococci. The API Staph-Ident system, a biochemical and chromogenic substrate micromethod, correctly identified 95 of 120 (79.2%) clinical isolates of coagulase-negative staphylococci after 5 h of incubation. The DMS Staph-Trac system, a miniaturized biochemical test system which requires a 24-h incubation period, correctly identified 106 (88.3%) of the same isolates. Both commercial systems were similar in cost and amount of technologist time required to inoculate and read each system. The clinical value of routine species identification of coagulase-negative staphylococci has not yet been established. The decision by clinical laboratories of whether to adopt this practice will be greatly facilitated by the availability of commercial kit systems which are both rapid and accurate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E., Stratton C. W., Patterson L. S., Evans M. E., Hodges R. L. Comparison of the Staph-Ident system with a conventional method for species identification of urine and blood isolates of coagulase-negative staphylococci. J Clin Microbiol. 1983 Mar;17(3):516–520. doi: 10.1128/jcm.17.3.516-520.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Archer G. L. Antimicrobial susceptibility and selection of resistance among Staphylococcus epidermidis isolates recovered from patients with infections of indwelling foreign devices. Antimicrob Agents Chemother. 1978 Sep;14(3):353–359. doi: 10.1128/aac.14.3.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BAIRD-PARKER A. C. A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963 Mar;30:409–427. doi: 10.1099/00221287-30-3-409. [DOI] [PubMed] [Google Scholar]
  4. BAIRD-PARKER A. C. THE CLASSIFICATION OF STAPHYLOCOCCI AND MICROCOCCI FROM WORLD-WIDE SOURCES. J Gen Microbiol. 1965 Mar;38:363–387. doi: 10.1099/00221287-38-3-363. [DOI] [PubMed] [Google Scholar]
  5. Brun Y., Fleurette J., Forey F. Micromethod for biochemical identification of coagulase-negative staphylococci. J Clin Microbiol. 1978 Nov;8(5):503–508. doi: 10.1128/jcm.8.5.503-508.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doern G. V., Earls J. E., Jeznach P. A., Parker D. S. Species identification and biotyping of staphylococci by the API staph-ident system. J Clin Microbiol. 1983 Feb;17(2):260–263. doi: 10.1128/jcm.17.2.260-263.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eng R. H., Wang C., Person A., Kiehn T. E., Armstrong D. Species identification of coagulase-negative staphylococcal isolates from blood cultures. J Clin Microbiol. 1982 Mar;15(3):439–442. doi: 10.1128/jcm.15.3.439-442.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gemmell C. G., Dawson J. E. Identification of coagulase-negative staphylococci with the API staph system. J Clin Microbiol. 1982 Nov;16(5):874–877. doi: 10.1128/jcm.16.5.874-877.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gillespie W. A., Sellin M. A., Gill P., Stephens M., Tuckwell L. A., Hilton A. L. Urinary tract infection in young women, with special reference to Staphylococcus saprophyticus. J Clin Pathol. 1978 Apr;31(4):348–350. doi: 10.1136/jcp.31.4.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kloos W. E. Natural populations of the genus Staphylococcus. Annu Rev Microbiol. 1980;34:559–592. doi: 10.1146/annurev.mi.34.100180.003015. [DOI] [PubMed] [Google Scholar]
  11. Kloos W. E., Schleifer K. H. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol. 1975 Jan;1(1):82–88. doi: 10.1128/jcm.1.1.82-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kloos W. E., Wolfshohl J. F. Identification of Staphylococcus species with the API STAPH-IDENT system. J Clin Microbiol. 1982 Sep;16(3):509–516. doi: 10.1128/jcm.16.3.509-516.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Liekweg W. G., Jr, Greenfield L. J. Vascular prosthetic infections: collected experience and results of treatment. Surgery. 1977 Mar;81(3):335–342. [PubMed] [Google Scholar]
  14. Marrie T. J., Kwan C., Noble M. A., West A., Duffield L. Staphylococcus saprophyticus as a cause of urinary tract infections. J Clin Microbiol. 1982 Sep;16(3):427–431. doi: 10.1128/jcm.16.3.427-431.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pead L., Crump J., Maskell R. Staphylococci as urinary pathogens. J Clin Pathol. 1977 May;30(5):427–431. doi: 10.1136/jcp.30.5.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rubin J., Rogers W. A., Taylor H. M., Everett E. D., Prowant B. F., Fruto L. V., Nolph K. D. Peritonitis during continuous ambulatory peritoneal dialysis. Ann Intern Med. 1980 Jan;92(1):7–13. doi: 10.7326/0003-4819-92-1-7. [DOI] [PubMed] [Google Scholar]
  17. Schoenbaum S. C., Gardner P., Shillito J. Infections of cerebrospinal fluid shunts: epidemiology, clinical manifestations, and therapy. J Infect Dis. 1975 May;131(5):543–552. doi: 10.1093/infdis/131.5.543. [DOI] [PubMed] [Google Scholar]
  18. Sewell C. M., Clarridge J. E., Young E. J., Guthrie R. K. Clinical significance of coagulase-negative staphylococci. J Clin Microbiol. 1982 Aug;16(2):236–239. doi: 10.1128/jcm.16.2.236-239.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tselenis-Kotsowilis A. D., Koliomichalis M. P., Papavassiliou J. T. Acute pyelonephritis caused by Staphylococcus xylosus. J Clin Microbiol. 1982 Sep;16(3):593–594. doi: 10.1128/jcm.16.3.593-594.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wallmark G., Arremark I., Telander B. Staphylococcus saprophyticus: a frequent cause of acute urinary tract infection among female outpatients. J Infect Dis. 1978 Dec;138(6):791–797. doi: 10.1093/infdis/138.6.791. [DOI] [PubMed] [Google Scholar]
  21. Wilson P. D., Jr, Salvati E. A., Aglietti P., Kutner L. J. The problem of infection in endoprosthetic surgery of the hip joint. Clin Orthop Relat Res. 1973 Oct;(96):213–221. [PubMed] [Google Scholar]

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