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. 1984 Jan;19(1):73–76. doi: 10.1128/jcm.19.1.73-76.1984

Evaluation of PPLO, A7B, E, and NYC agar media for the isolation of Ureaplasma urealyticum and Mycoplasma species from the genital tract.

D M Yajko, E Balston, D Wood, R L Sweet, W K Hadley
PMCID: PMC270982  PMID: 6690468

Abstract

Four agar media (PPLO, NYC, A7B, and E) which are commonly used for the isolation of urogenital tract Mycoplasma species and Ureaplasma urealyticum were compared by culturing swabs of the endocervix of 334 pregnant women on all four media. To permit growth of both Mycoplasma and U. urealyticum, selective ingredients were omitted from the media tested. A7B and E agar were both satisfactory for the isolation of Mycoplasma species, recovering 92 and 82%, respectively, of all Mycoplasma species isolated. Only A7B agar was satisfactory for U. urealyticum isolation, recovering 96% of all isolates. Several modifications of PPLO, NYC, and E agar failed to significantly improve recovery of U. urealyticum on these media. A7B agar was clearly superior to all other media tested in terms of recovery rate, typical appearance of colonies, and ease of reading. A7B can be used for the isolation of both U. urealyticum and Mycoplasma species from urogenital sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun P., Klein J. O., Lee Y. H., Kass E. H. Methodologic investigations and prevalence of genital mycoplasmas in pregnancy. J Infect Dis. 1970 Apr;121(4):391–400. doi: 10.1093/infdis/121.4.391. [DOI] [PubMed] [Google Scholar]
  2. Braun P., Lee Y. H., Klein J. O., Marcy S. M., Klein T. A., Charles D., Levy P., Kass E. H. Birth weight and genital mycoplasmas in pregnancy. N Engl J Med. 1971 Jan 28;284(4):167–171. doi: 10.1056/NEJM197101282840401. [DOI] [PubMed] [Google Scholar]
  3. Cassell G. H., Younger J. B., Brown M. B., Blackwell R. E., Davis J. K., Marriott P., Stagno S. Microbiologic study of infertile women at the time of diagnostic laparoscopy. Association of Ureaplasma urealyticum with a defined subpopulation. N Engl J Med. 1983 Mar 3;308(9):502–505. doi: 10.1056/NEJM198303033080906. [DOI] [PubMed] [Google Scholar]
  4. Embree J. E., Krause V. W., Embil J. A., MacDonald S. Placental infection with Mycoplasma homonis and Ureaplasma urealyticum: clinical correlation. Obstet Gynecol. 1980 Oct;56(4):475–481. [PubMed] [Google Scholar]
  5. Faur Y. C., Weisburd M. H., Wilson M. E. A new medium for the isolation of pathogenic Neisseria (NYC medium). II. Effect of amphotericin B and trimethoprim lactate on selectivity. Health Lab Sci. 1973 Apr;10(2):55–60. [PubMed] [Google Scholar]
  6. Faur Y. C., Weisburd M. H., Wilson M. E., May P. S. A new medium for the isolation of pathogenic Neisseria (NYC medium). I. Formulation and comparisons with standard media. Health Lab Sci. 1973 Apr;10(2):44–54. [PubMed] [Google Scholar]
  7. Faur Y. C., Weisburd M. H., Wlson M. E., May P. S. NYC medium for simultaneous isolation of Neisseria gonorrhoeae, large-colony mycoplasmas, and T-mycoplasmas. Appl Microbiol. 1974 Jun;27(6):1041–1045. doi: 10.1128/am.27.6.1041-1045.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gnarpe H., Friberg J. Mycoplasma and human reproductive failure. I. The occurrence of different Mycoplasmas in couples with reproductive failure. Am J Obstet Gynecol. 1972 Nov 15;114(6):727–731. [PubMed] [Google Scholar]
  9. Kundsin R. B., Driscoll S. G., Pelletier P. A. Ureaplasma urealyticum incriminated in perinatal morbidity and mortality. Science. 1981 Jul 24;213(4506):474–475. doi: 10.1126/science.7244646. [DOI] [PubMed] [Google Scholar]
  10. Kundsin R. B., Parreno A., Poulin S. Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens. J Clin Microbiol. 1978 Oct;8(4):445–453. doi: 10.1128/jcm.8.4.445-453.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leland D. S., Lapworth M. A., Jones R. B., French M. L. Comparative evaluation of media for isolation of ureaplasma urealyticum and genital mycoplasma species. J Clin Microbiol. 1982 Oct;16(4):709–714. doi: 10.1128/jcm.16.4.709-714.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mårdh P. A., Weström L. Tubal and cervical cultures in acute salpingitis with special reference to Mycoplasma hominis and T-strain mycoplasmas. Br J Vener Dis. 1970 Jun;46(3):179–186. [PMC free article] [PubMed] [Google Scholar]
  13. Rottem S., Pfendt E. A., Hayflick L. Sterol requirements of T-strain mycoplasmas. J Bacteriol. 1971 Jan;105(1):323–330. doi: 10.1128/jb.105.1.323-330.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SHEPARD M. C., LUNCEFORD C. D. EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS. J Bacteriol. 1965 Feb;89:265–270. doi: 10.1128/jb.89.2.265-270.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shepard M. C., Combs R. S. Enhancement of Ureaplasma urealyticum growth on a differential agar medium (A7B) by a polyamine, putrescine. J Clin Microbiol. 1979 Dec;10(6):931–933. doi: 10.1128/jcm.10.6.931-933.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shepard M. C., Lunceford C. D. Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol. 1976 Jun;3(6):613–625. doi: 10.1128/jcm.3.6.613-625.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shepard M. C. Nongonococcal urethritis associated with human strains of "T" mycoplasmas. JAMA. 1970 Feb 23;211(8):1335–1340. [PubMed] [Google Scholar]
  18. Stray-Pedersen B., Eng J., Reikvam T. M. Uterine T-mycoplasma colonization in reproductive failure. Am J Obstet Gynecol. 1978 Feb 1;130(3):307–311. [PubMed] [Google Scholar]
  19. Toth A., Lesser M. L., Brooks C., Labriola D. Subsequent pregnancies among 161 couples treated for T-mycoplasma genital-tract infection. N Engl J Med. 1983 Mar 3;308(9):505–507. doi: 10.1056/NEJM198303033080907. [DOI] [PubMed] [Google Scholar]

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