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. 1984 Feb;19(2):175–180. doi: 10.1128/jcm.19.2.175-180.1984

Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species.

P C Turnbull, J V Lee, M D Miliotis, S Van de Walle, H J Koornhof, L Jeffery, T N Bryant
PMCID: PMC271012  PMID: 6699147

Abstract

A total of 19 of 20 (95%) strains of Aeromonas hydrophila biovar hydrophila and 16 of 17 (94%) strains of Aeromonas sobria isolated from a variety of clinical and environmental sources were found to be enterotoxin positive. Only 2 of 18 (11%) A. hydrophila biovar anaerogenes and 2 of 13 (15%) unidentified Aeromonas strains from a similar variety of sources produced enterotoxin. No association was apparent between the source of isolation, in particular diarrheal stools, and enterotoxigenicity; 41% of the isolates from diarrheal stools were enterotoxin negative. A strong correlation was noted between ability to produce enterotoxin and positive results in six characters: lysine decarboxylase and Voges-Proskauer reactions, production of gas from glucose, gluconate oxidation, xanthine hydrolysis, and hemolysis of human erythrocytes. In the majority of cases (35 of 39 strains), enterotoxigenicity was detected using cell-free filtrates of brain heart infusion broth cultures grown at 36 degrees C for 15; however, the other four positive isolates were detected after growth in the same broth at 30 degrees C or in Casamino Acids-yeast extract broth at 30 or 37 degrees C. It is recommended that for enterotoxin tests, strains should be grown in both media at both temperatures. The infant mouse test was found to be a simple and reliable method for detection of the enterotoxin. The toxin proved to be heat labile and not neutralized by cholera antitoxin.

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Selected References

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  1. Annapurna E., Sanyal S. C. Enterotoxicity of Aeromonas hydrophila. J Med Microbiol. 1977 Aug;10(3):317–323. doi: 10.1099/00222615-10-3-317. [DOI] [PubMed] [Google Scholar]
  2. Burke V., Robinson J., Atkinson H. M., Gracey M. Biochemical characteristics of enterotoxigenic Aeromonas spp. J Clin Microbiol. 1982 Jan;15(1):48–52. doi: 10.1128/jcm.15.1.48-52.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burke V., Robinson J., Berry R. J., Gracey M. Detection of enterotoxins of Aeromonas hydrophila by a suckling-mouse test. J Med Microbiol. 1981 Nov;14(4):401–408. doi: 10.1099/00222615-14-4-401. [DOI] [PubMed] [Google Scholar]
  4. Cumberbatch N., Gurwith M. J., Langston C., Sack R. B., Brunton J. L. Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease. Infect Immun. 1979 Mar;23(3):829–837. doi: 10.1128/iai.23.3.829-837.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Daily O. P., Joseph S. W., Coolbaugh J. C., Walker R. I., Merrell B. R., Rollins D. M., Seidler R. J., Colwell R. R., Lissner C. R. Association of Aeromonas sobria with human infection. J Clin Microbiol. 1981 Apr;13(4):769–777. doi: 10.1128/jcm.13.4.769-777.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Evans D. J., Jr, Evans D. G., Gorbach S. L. Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Nov;8(5):725–730. doi: 10.1128/iai.8.5.725-730.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Finkelstein R. A., Atthasampunna P., Chulasamaya M., Charunmethee P. Pathogenesis of experimental cholera: biologic ativities of purified procholeragen A. J Immunol. 1966 Mar;96(3):440–449. [PubMed] [Google Scholar]
  8. Gracey M., Burke V., Robinson J. Aeromonas-associated gastroenteritis. Lancet. 1982 Dec 11;2(8311):1304–1306. doi: 10.1016/s0140-6736(82)91510-0. [DOI] [PubMed] [Google Scholar]
  9. Gracey M., Burke V., Rockhill R. C., Suharyono, Sunoto Aeromonas species as enteric pathogens. Lancet. 1982 Jan 23;1(8265):223–224. doi: 10.1016/s0140-6736(82)90787-5. [DOI] [PubMed] [Google Scholar]
  10. Hubbard G. B. Aeromonas hydrophila infection in Xenopus laevis. Lab Anim Sci. 1981 Jun;31(3):297–300. [PubMed] [Google Scholar]
  11. James C., Dibley M., Burke V., Robinson J., Gracey M. Immunological cross-reactivity of enterotoxins of Aeromonas hydrophila and cholera toxin. Clin Exp Immunol. 1982 Jan;47(1):34–42. [PMC free article] [PubMed] [Google Scholar]
  12. Johnson W. M., Lior H. Cytotoxicity and suckling mouse reactivity of Aeromonas hydrophila isolated from human sources. Can J Microbiol. 1981 Oct;27(10):1019–1027. doi: 10.1139/m81-159. [DOI] [PubMed] [Google Scholar]
  13. Jánossy G., Tarján V. Enterotoxigenicity of aeromonas strains in suckling mice. Acta Microbiol Acad Sci Hung. 1980;27(1):63–69. [PubMed] [Google Scholar]
  14. Kaper J. B., Lockman H., Colwell R. R., Joseph S. W. Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary. J Appl Bacteriol. 1981 Apr;50(2):359–377. doi: 10.1111/j.1365-2672.1981.tb00900.x. [DOI] [PubMed] [Google Scholar]
  15. Ljungh A., Eneroth P., Wadström T. Cytotonic enterotoxin from Aeromonas hydrophila. Toxicon. 1982;20(4):787–794. doi: 10.1016/0041-0101(82)90126-x. [DOI] [PubMed] [Google Scholar]
  16. Ljungh A., Kronevi T. Aeromonas hydrophila toxins - intestinal fluid accumulation and mucosal injury in animal models. Toxicon. 1982;20(2):397–407. doi: 10.1016/0041-0101(82)90002-2. [DOI] [PubMed] [Google Scholar]
  17. Ljungh A., Wadström T. Aeromonas toxins. Pharmacol Ther. 1981;15(3):339–354. doi: 10.1016/0163-7258(81)90049-8. [DOI] [PubMed] [Google Scholar]
  18. Ljungh A., Wretlind B., Möllby R. Separation and characterization of enterotoxin and two haemolysins from Aeromonas hydrophila. Acta Pathol Microbiol Scand B. 1981 Dec;89(6):387–397. doi: 10.1111/j.1699-0463.1981.tb00205_89b.x. [DOI] [PubMed] [Google Scholar]
  19. MØLLER V. Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta Pathol Microbiol Scand. 1955;36(2):158–172. doi: 10.1111/j.1699-0463.1955.tb04583.x. [DOI] [PubMed] [Google Scholar]
  20. Olivier G., Lallier R., Larivière S. A toxigenic profile of Aeromonas hydrophila and Aeromonas sobria isolated from fish. Can J Microbiol. 1981 Mar;27(3):330–333. doi: 10.1139/m81-050. [DOI] [PubMed] [Google Scholar]
  21. Pitarangsi C., Echeverria P., Whitmire R., Tirapat C., Formal S., Dammin G. J., Tingtalapong M. Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides: prevalence among individuals with and without diarrhea in Thailand. Infect Immun. 1982 Feb;35(2):666–673. doi: 10.1128/iai.35.2.666-673.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Popoff M., Véron M. A taxonomic study of the Aeromonas hydrophila-Aeromonas punctata group. J Gen Microbiol. 1976 May;94(1):11–22. doi: 10.1099/00221287-94-1-11. [DOI] [PubMed] [Google Scholar]
  23. Schubert R. H. Das Vorkommen der Aeromonaden in oberirdischen Gewässern. Arch Hyg Bakteriol. 1967 Mar;150(7):688–708. [PubMed] [Google Scholar]
  24. Wadström T., Ljungh A., Wretlind B. Enterotoxin, haemolysin and cytotoxic protein in Aeromonas hydrophila from human infections. Acta Pathol Microbiol Scand B. 1976 Apr;84(2):112–114. doi: 10.1111/j.1699-0463.1976.tb01911.x. [DOI] [PubMed] [Google Scholar]
  25. West P. A., Lee J. V., Bryant T. N. A numerical taxonomic study of species of Vibrio isolated from the aquatic environment and birds in Kent, England. J Appl Bacteriol. 1983 Oct;55(2):263–282. doi: 10.1111/j.1365-2672.1983.tb01324.x. [DOI] [PubMed] [Google Scholar]

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