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. 1984 Feb;19(2):194–196. doi: 10.1128/jcm.19.2.194-196.1984

Evaluation of three simple and rapid immunological tests for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

D Sen, M R Saha, S C Pal
PMCID: PMC271016  PMID: 6421871

Abstract

Three simple immunological tests, the modified Elek (Biken) test, the modified staphylococcal coagglutination test, and the rapid GM1-horseradish peroxidase-enzyme-linked immunosorbent assay have been evaluated for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli. Of the 100 coded E. coli strains tested, 94 gave consistent results with all the three immunological tests; a discrepancy was observed in only 6 strains. Identical results were obtained when the Biken test was conducted with complete and incomplete Biken kits (Meguro Institute Ltd., Osaka, Japan). All three immunological tests evaluated in this study were found to be sensitive and simple and can be easily adopted by any laboratory for detection of heat-labile enterotoxins of enterotoxigenic E. coli strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brill B. M., Wasilauskas B. L., Richardson S. H. Adaptation of the staphylococcal coagglutination technique for detection of heat-labile enterotoxin of Escherichia coli. J Clin Microbiol. 1979 Jan;9(1):49–55. doi: 10.1128/jcm.9.1.49-55.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bäck E., Svennerholm A. M., Holmgren J., Möllby R. Evaluation of a ganglioside immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1979 Dec;10(6):791–795. doi: 10.1128/jcm.10.6.791-795.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  4. Evans D. J., Jr, Evans D. G. Direct serological assay for the heat-labile enterotoxin of Escherichia coli, using passive immune hemolysis. Infect Immun. 1977 May;16(2):604–609. doi: 10.1128/iai.16.2.604-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans D. J., Jr, Evans D. G., Gorbach S. L. Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Nov;8(5):725–730. doi: 10.1128/iai.8.5.725-730.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finkelstein R. A., Yang Z. Rapid test for identification of heat-labile enterotoxin-producing Escherichia coli colonies. J Clin Microbiol. 1983 Jul;18(1):23–28. doi: 10.1128/jcm.18.1.23-28.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Greenberg H. B., Sack D. A., Rodriguez W., Sack R. B., Wyatt R. G., Kalica A. R., Horswood R. L., Chanock R. M., Kapikian A. Z. Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin. Infect Immun. 1977 Sep;17(3):541–545. doi: 10.1128/iai.17.3.541-545.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Honda T., Arita M., Takeda Y., Miwatani T. Further evaluation of the Biken test (modified Elek test) for detection of enterotoxigenic Escherichia coli producing heat-labile enterotoxin and application of the test to sampling of heat-stable enterotoxin. J Clin Microbiol. 1982 Jul;16(1):60–62. doi: 10.1128/jcm.16.1.60-62.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moon H. W., Whipp S. C., Engstrom G. W., Baetz A. L. Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine. J Infect Dis. 1970 Feb;121(2):182–187. doi: 10.1093/infdis/121.2.182. [DOI] [PubMed] [Google Scholar]
  11. Rönnberg B., Wadström T. Rapid detection by a coagglutination test of heat-labile enterotoxin in cell lysates from blood agar-grown Escherichia coli. J Clin Microbiol. 1983 Jun;17(6):1021–1025. doi: 10.1128/jcm.17.6.1021-1025.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sack D. A., Huda S., Neogi P. K., Daniel R. R., Spira W. M. Microtiter ganglioside enzyme-linked immunosorbent assay for vibrio and Escherichia coli heat-labile enterotoxins and antitoxin. J Clin Microbiol. 1980 Jan;11(1):35–40. doi: 10.1128/jcm.11.1.35-40.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Svennerholm A. M., Wiklund G. Rapid GM1-enzyme-linked immunosorbent assay with visual reading for identification of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1983 Apr;17(4):596–600. doi: 10.1128/jcm.17.4.596-600.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yolken R. H., Greenberg H. B., Merson M. H., Sack R. B., Kapikian A. Z. Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1977 Nov;6(5):439–444. doi: 10.1128/jcm.6.5.439-444.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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