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. 1984 Feb;19(2):279–280. doi: 10.1128/jcm.19.2.279-280.1984

Five-hour novobiocin test for differentiation of coagulase-negative staphylococci.

B J Harrington, J M Gaydos
PMCID: PMC271037  PMID: 6699151

Abstract

A 5-h broth disk test, read visually for growth or no growth to determine resistance of coagulase-negative staphylococci to 1.6 micrograms of novobiocin per ml, was evaluated as a rapid test for the presumptive identification of Staphylococcus saprophyticus. The correlation with an overnight disk diffusion test was 100%.

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Selected References

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  1. Almeida R. J., Jorgensen J. H. Rapid determination of novobiocin resistance of coagulase-negative staphylococci with the MS-2 system. J Clin Microbiol. 1983 Mar;17(3):558–560. doi: 10.1128/jcm.17.3.558-560.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Almeida R. J., Jorgensen J. H. Use of Mueller-Hinton agar to determine novobiocin susceptibility of coagulase-negative staphylococci. J Clin Microbiol. 1982 Dec;16(6):1155–1156. doi: 10.1128/jcm.16.6.1155-1156.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson J. D., Clarke A. M., Anderson M. E., Isaac-Renton J. L., McLoughlin M. G. Urinary tract infections due to Staphylococcus saprophyticus biotype 3. Can Med Assoc J. 1981 Feb 15;124(4):415–418. [PMC free article] [PubMed] [Google Scholar]
  4. Bailey R. R. Significance of coagulase-negative Staphylococcus in urine. J Infect Dis. 1973 Feb;127(2):179–182. doi: 10.1093/infdis/127.2.179. [DOI] [PubMed] [Google Scholar]
  5. Gillespie W. A., Sellin M. A., Gill P., Stephens M., Tuckwell L. A., Hilton A. L. Urinary tract infection in young women, with special reference to Staphylococcus saprophyticus. J Clin Pathol. 1978 Apr;31(4):348–350. doi: 10.1136/jcp.31.4.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldstein J., Schulman R., Kelley E., McKinley G., Fung J. Effect of different media on determination of novobiocin resistance for differentiation of coagulase-negative staphylococci. J Clin Microbiol. 1983 Sep;18(3):592–595. doi: 10.1128/jcm.18.3.592-595.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hovelius B., Mårdh P. A. On the diagnosis of coagulase-negative staphylococci with emphasis on Staphylococcus saprophyticus. Acta Pathol Microbiol Scand B. 1977 Dec;85B(6):427–434. doi: 10.1111/j.1699-0463.1977.tb01998.x. [DOI] [PubMed] [Google Scholar]
  8. John J. F., Jr, Gramling P. K., O'Dell N. M. Species identification of coagulase-negative staphylococci from urinary tract isolates. J Clin Microbiol. 1978 Oct;8(4):435–437. doi: 10.1128/jcm.8.4.435-437.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jordan P. A., Iravani A., Richard G. A., Baer H. Urinary tract infection caused by Staphylococcus saprophyticus. J Infect Dis. 1980 Oct;142(4):510–515. doi: 10.1093/infdis/142.4.510. [DOI] [PubMed] [Google Scholar]
  10. Kloos W. E., Schleifer K. H. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol. 1975 Jan;1(1):82–88. doi: 10.1128/jcm.1.1.82-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leighton P. M., White M. Rapid determination of novobiocin susceptibility for the identification of Staphylococcus saprophyticus. Diagn Microbiol Infect Dis. 1983 Sep;1(3):261–264. doi: 10.1016/0732-8893(83)90026-3. [DOI] [PubMed] [Google Scholar]
  12. Lewis J. F., Brake S. R., Anderson D. J., Vredeveld G. N. Urinary tract infection due to coagulase-negative staphylococcus. Am J Clin Pathol. 1982 Jun;77(6):736–739. doi: 10.1093/ajcp/77.6.736. [DOI] [PubMed] [Google Scholar]
  13. Marrie T. J., Kwan C. Antimicrobial susceptibility of Staphylococcus saprophyticus and urethral staphylococci. Antimicrob Agents Chemother. 1982 Sep;22(3):395–397. doi: 10.1128/aac.22.3.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mitchell R. G. Classification of Staphylococcus albus strains isolated from the urinary tract. J Clin Pathol. 1968 Jan;21(1):93–96. doi: 10.1136/jcp.21.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Namavar F., De Graaff J., De With C., MacLaren D. M. Novobiocin resistance and virulence of strains of Staphylococcus saprophyticus isolated from urine and skin. J Med Microbiol. 1978 Aug;11(3):243–248. doi: 10.1099/00222615-11-3-243. [DOI] [PubMed] [Google Scholar]
  16. Pead L., Crump J., Maskell R. Staphylococci as urinary pathogens. J Clin Pathol. 1977 May;30(5):427–431. doi: 10.1136/jcp.30.5.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wallmark G., Arremark I., Telander B. Staphylococcus saprophyticus: a frequent cause of acute urinary tract infection among female outpatients. J Infect Dis. 1978 Dec;138(6):791–797. doi: 10.1093/infdis/138.6.791. [DOI] [PubMed] [Google Scholar]

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