Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1984 Feb;19(2):291–293. doi: 10.1128/jcm.19.2.291-293.1984

Characterization of Pseudomonas cepacia isolates from patients with cystic fibrosis.

A I McKevitt, D E Woods
PMCID: PMC271041  PMID: 6699152

Abstract

Pseudomonas cepacia infections which follow a fulminant course and which include septicemia are being reported with increasing frequency from cystic fibrosis patients. Forty-eight P. cepacia isolates from cystic fibrosis patients were screened for production of potential virulence factors. A majority of strains tested produced protease and lipase. Eleven strains harbored plasmids of approximate molecular weights in the range 50 X 10(6) to 100 X 10(6). Twenty-two strains produced a smooth lipopolysaccharide. Studies are presently under way to determine the role of these potential virulence factors in the pathogenesis of P. cepacia disease.

Full text

PDF
291

Images in this article

292Image
on p.292

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bjorn M. J., Pavlovskis O. R., Thompson M. R., Iglewski B. H. Production of exoenzyme S during Pseudomonas aeruginosa infections of burned mice. Infect Immun. 1979 Jun;24(3):837–842. doi: 10.1128/iai.24.3.837-842.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chung D. W., Collier R. J. Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa. Infect Immun. 1977 Jun;16(3):832–841. doi: 10.1128/iai.16.3.832-841.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ESSELMANN M. T., LIU P. V. Lecithinase production by gramnegative bacteria. J Bacteriol. 1961 Jun;81:939–945. doi: 10.1128/jb.81.6.939-945.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gonzalez C. F., Vidaver A. K. Bacteriocin, plasmid and pectolytic diversity in Pseudomonas cepacia of clinical and plant origin. J Gen Microbiol. 1979 Jan;110(1):161–170. doi: 10.1099/00221287-110-1-161. [DOI] [PubMed] [Google Scholar]
  5. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hudson A. R., Kilburn K. H., Halprin G. M., McKenzie W. N. Granulocyte recruitment to airways exposed to endotoxin aerosols. Am Rev Respir Dis. 1977 Jan;115(1):89–95. doi: 10.1164/arrd.1977.115.1.89. [DOI] [PubMed] [Google Scholar]
  7. Liu P. V. Extracellular toxins of Pseudomonas aeruginosa. J Infect Dis. 1974 Nov;130 (Suppl)(0):S94–S99. doi: 10.1093/infdis/130.supplement.s94. [DOI] [PubMed] [Google Scholar]
  8. Nolan G., Moivor P., Levison H., Fleming P. C., Corey M., Gold R. Antibiotic prophylaxis in cystic fibrosis: inhaled cephaloridine as an adjunct to oral cloxacillin. J Pediatr. 1982 Oct;101(4):626–630. doi: 10.1016/s0022-3476(82)80726-9. [DOI] [PubMed] [Google Scholar]
  9. Ohman D. E., Sadoff J. C., Iglewski B. H. Toxin A-deficient mutants of Pseudomonas aeruginosa PA103: isolation and characterization. Infect Immun. 1980 Jun;28(3):899–908. doi: 10.1128/iai.28.3.899-908.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rosenstein B. J., Hall D. E. Pneumonia and septicemia due to Pseudomonas cepacia in a patient with cystic fibrosis. Johns Hopkins Med J. 1980 Nov;147(5):188–189. [PubMed] [Google Scholar]
  11. Sokol P. A., Ohman D. E., Iglewski B. H. A more sensitive plate assay for detection of protease production by Pseudomanas aeruginosa. J Clin Microbiol. 1979 Apr;9(4):538–540. doi: 10.1128/jcm.9.4.538-540.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vasil M. L., Kabat D., Iglewski B. H. Structure-activity relationships of an exotoxin of Pseudomonas aeruginosa. Infect Immun. 1977 Apr;16(1):353–361. doi: 10.1128/iai.16.1.353-361.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weinbaum G., Marco V., Ikeda T., Mass B., Meranze D. R., Kimbel P. Enzymatic production of experimental emphysema in the dog. Route of exposure. Am Rev Respir Dis. 1974 Mar;109(3):351–357. doi: 10.1164/arrd.1974.109.3.351. [DOI] [PubMed] [Google Scholar]
  14. Williams J. A., Yeggy J. P., Field C. C., Markovetz A. J. Resistance plasmids in Pseudomonas cepacia 4G9. J Bacteriol. 1979 Dec;140(3):1017–1022. doi: 10.1128/jb.140.3.1017-1022.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wood R. E., Boat T. F., Doershuk C. F. Cystic fibrosis. Am Rev Respir Dis. 1976 Jun;113(6):833–878. doi: 10.1164/arrd.1976.113.6.833. [DOI] [PubMed] [Google Scholar]
  16. Woods D. E., Cryz S. J., Friedman R. L., Iglewski B. H. Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infections of rats. Infect Immun. 1982 Jun;36(3):1223–1228. doi: 10.1128/iai.36.3.1223-1228.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES