Abstract
In a comparison of 1,368 positive blood cultures, a vented Roche Septi-Chek (V-RSC) blood culture bottle was superior to an unvented tryptic soy broth-containing bottle (Difco) for the recovery of all aerobic and facultatively anaerobic microorganisms. Anaerobic bacteria were recovered more frequently and earlier in the unvented tryptic soy broth-containing bottle. A separate comparison of 529 positive blood cultures was conducted to examine the performance of the V-RSC bottle with that of a vented brain heart infusion biphasic medium. The V-RSC bottle recovered significantly more isolates of Enterobacteriaceae and of anaerobic bacteria than did the vented brain heart infusion biphasic medium. The V-RSC bottle is a reliable blood culture system for all aerobic and facultatively anaerobic microorganisms. Because of its suboptimal recovery of anaerobic bacteria, it is recommended that the V-RSC bottle be used in combination with an unvented vacuum blood culture bottle.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hall M. M., Mueske C. A., Ilstrup D. M., Washington J. A., 2nd Evaluation of a biphasic medium for blood cultures. J Clin Microbiol. 1979 Nov;10(5):673–676. doi: 10.1128/jcm.10.5.673-676.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henry N. K., McLimans C. A., Wright A. J., Thompson R. L., Wilson W. R., Washington J. A., 2nd Microbiological and clinical evaluation of the isolator lysis-centrifugation blood culture tube. J Clin Microbiol. 1983 May;17(5):864–869. doi: 10.1128/jcm.17.5.864-869.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfaller M. A., Sibley T. K., Westfall L. M., Hoppe-Bauer J. E., Keating M. A., Murray P. R. Clinical laboratory comparison of a slide blood culture system with a conventional broth system. J Clin Microbiol. 1982 Sep;16(3):525–530. doi: 10.1128/jcm.16.3.525-530.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]