Abstract
An enzyme immunoassay (EIA; Gonozyme, Abbott Laboratories) for the antigenic detection of Neisseria gonorrhoeae in endocervical or urethral specimens was evaluated. EIA results were compared with results of conventional culture tests for N. gonorrhoeae. Specimens from 208 males (113 culture positive) and 252 females (72 culture positive) were tested. The sensitivity and specificity of EIA for specimens from males were 97.3 and 95.8%, respectively. The sensitivity and specificity of EIA for specimens from females were 79.2 and 87.2%, respectively.
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- Aardoom H. A., de Hoop D., Iserief C. O., Michel M. F., Stolz E. Detection of Neisseria gonorrhoeae antigen by a solid-phase enzyme immunoassay. Br J Vener Dis. 1982 Dec;58(6):359–362. doi: 10.1136/sti.58.6.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Caldwell J. G., Price E. V., Pazin G. J., Cornelius C. E., 3rd Sensitivity and reproducibility of Thayer-Martin culture mddium in diagnosing gonorrhea in women. Am J Obstet Gynecol. 1971 Feb 1;109(3):463–468. doi: 10.1016/0002-9378(71)90346-2. [DOI] [PubMed] [Google Scholar]
- Crawford C., Knapp J. S., Hale J., Holmes K. K. Asymptomatic gonorrhea in men: caused by gonococci with unique nutritional requirements. Science. 1977 Jun 17;196(4296):1352–1353. doi: 10.1126/science.405742. [DOI] [PubMed] [Google Scholar]
- Danielsson D., Moi H., Forslin L. Diagnosis of urogenital gonorrhoea by detecting gonococcal antigen with a solid phase enzyme immunoassay (Gonozyme). J Clin Pathol. 1983 Jun;36(6):674–677. doi: 10.1136/jcp.36.6.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
- Lewis J. S., Martin J. E., Jr Evaluation of the phadebact gonococcus test, a coagglutination procedure for confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1980 Feb;11(2):153–156. doi: 10.1128/jcm.11.2.153-156.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lowe T. L., Kraus S. J. Quantitation of Neisseria gonorrhoeae from women with gonorrhea. J Infect Dis. 1976 Jun;133(6):621–626. doi: 10.1093/infdis/133.6.621. [DOI] [PubMed] [Google Scholar]
- Mirrett S., Reller L. B., Knapp J. S. Neisseria gonorrhoeae strains inhibited by vancomycin in selective media and correlation with auxotype. J Clin Microbiol. 1981 Jul;14(1):94–99. doi: 10.1128/jcm.14.1.94-99.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Norins L. C. The case for gonococcal serology. J Infect Dis. 1974 Dec;130(6):677–679. doi: 10.1093/infdis/130.6.677. [DOI] [PubMed] [Google Scholar]
- Peacock W. L., Jr, Welch B. G., Martin J. E., Jr, Thayer J. D. Fluroescent antibody technique for identification of presumptively positive gonococcal cultures. Public Health Rep. 1968 Apr;83(4):337–339. [PMC free article] [PubMed] [Google Scholar]
- STUART R. D., TOSHACH S. R., PATSULA T. M. The problem of transport of specimens for culture of Gonococci. Can J Public Health. 1954 Feb;45(2):73–83. [PubMed] [Google Scholar]
- Schmale J. D., Martin J. E., Jr, Domescik G. Observations on the culture diagnosis of gonorrhea in women. JAMA. 1969 Oct 13;210(2):312–314. [PubMed] [Google Scholar]
- Symington D. A. Improved transport system for Neisseria gonorrhoeae in clinical specimens. J Clin Microbiol. 1975 Dec;2(6):498–503. doi: 10.1128/jcm.2.6.498-503.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]