Correction for Fujita et al., A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique

CELL BIOLOGY Correction for “A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique,” by Akikazu Fujita, Jinglei Cheng, Kumi Tauchi-Sato, Tadaomi Takenawa, and Toyoshi Fujimoto, which appeared in issue 23, June 9, 2009, of Proc Natl Acad Sci USA (106:9256–9261; first published May 22, 2009; 10.1073/pnas.0900216106).

The authors note that on page 9257, Figure 1 appeared incorrectly. This error does not affect the conclusions of the article. The corrected figure and its legend appear below.

Fig. 1.

Labeling of the liposome. (A) Outline of the method. Cells were rapidly frozen, freeze-fractured, and evaporated with carbon (C) and platinum/carbon (Pt/C) in vacuum. The replica of the split membrane was digested with SDS to remove noncast molecules and labeled by GST-PH. Both the cytoplasmic and exoplasmic halves of the membrane were examined. (B) Labeling of small unilamellar liposome replicas. Freeze-fracture replicas of liposomes containing 95 mol % of phosphatidylcholine (PC) and 5 mol % of phosphatidylinositol or a phosphoinositide were labeled. Only liposomes containing PI(4,5)P₂ were labeled intensely by GST-PH. A PH mutant, GST-PH(K30N, K32N), which does not bind PI(4,5)P₂, showed little labeling in the PI(4,5)P₂-containing liposome. (C) Quantification of the GST-PH labeling in the liposomes. The number of gold particles per 1 μm² of the liposome surface is shown (blue). The labeling on the convex (green) and concave (yellow) surfaces showed equivalent results.