Abstract
The Rapid Strep system (API System S.A., Montalieu-Vercieu, France) was evaluated, without additional tests, in the identification of 209 streptococci. Organisms included 59 beta-hemolytic, 36 group D, 24 Streptococcus pneumoniae, and 90 viridans group streptococci. The Rapid Strep system correctly identified to species level 69.5% of the beta-hemolytic strains, 100% of the group D strains, none of the S. pneumoniae strains, and 84.5% of the viridans group streptococci. The method provided excellent identification rates of groups A, B, and D but failed to differentiate between groups C and G. The method for preparation of suspensions for the Rapid Strep system, as initially recommended by the manufacturer, was responsible for the failure to identify S. pneumoniae. This method was subsequently modified to yield a heavier inoculum; all 10 pneumococcal strains tested with the revised inoculum method were correctly identified. Good identification rates of commonly encountered viridans strains were found. The Rapid Strep system represents a worthwhile advance in streptococcal species identification, especially for group D and viridans strains.
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- Appelbaum P. C., Jacobs M. R., Heald J. I., Palko W. M., Duffett A., Crist R., Naugle P. A. Comparative evaluation of the API 20S system and the automicrobic system gram-positive identification card for species identification of streptococci. J Clin Microbiol. 1984 Feb;19(2):164–168. doi: 10.1128/jcm.19.2.164-168.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ball L. C., Parker M. T. The cultural and biochemical characters of Streptococcus milleri strains isolated from human sources. J Hyg (Lond) 1979 Feb;82(1):63–78. doi: 10.1017/s002217240002547x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Facklam R. R. Physiological differentiation of viridans streptococci. J Clin Microbiol. 1977 Feb;5(2):184–201. doi: 10.1128/jcm.5.2.184-201.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keville M. W., Doern G. V. Comparison of the API 20S Streptococcus identification system with an immunorheophoresis procedure and two commercial latex agglutination tests for identifying beta-hemolytic streptococci. J Clin Microbiol. 1982 Jul;16(1):92–95. doi: 10.1128/jcm.16.1.92-95.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nachamkin I., Lynch J. R., Dalton H. P. Evaluation of a rapid system for species identification of alpha-hemolytic streptococci. J Clin Microbiol. 1982 Sep;16(3):521–524. doi: 10.1128/jcm.16.3.521-524.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ruoff K. L., Ferraro M. J., Jerz M. E., Kissling J. Automated identification of gram-positive bacteria. J Clin Microbiol. 1982 Dec;16(6):1091–1095. doi: 10.1128/jcm.16.6.1091-1095.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ruoff K. L., Kunz L. J. Use of the Rapid STREP system for identification of viridans streptococcal species. J Clin Microbiol. 1983 Nov;18(5):1138–1140. doi: 10.1128/jcm.18.5.1138-1140.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sands M., Sommers H. M., Rubin M. B. Speciation of the "viridans" streptococci. Am J Clin Pathol. 1982 Jul;78(1):78–80. doi: 10.1093/ajcp/78.1.78. [DOI] [PubMed] [Google Scholar]
- Waitkins S. A., Anderson D. R., Todd F. K. An evaluation of the API-STREP identification system. Med Lab Sci. 1981 Jan;38(1):35–39. [PubMed] [Google Scholar]
- Waitkins S. A., Ball L. C., Fraser C. A. Use of the API-ZYM system in rapid identification of alpha and non-haemolytic streptococci. J Clin Pathol. 1980 Jan;33(1):53–57. doi: 10.1136/jcp.33.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]