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. 1984 May;19(5):664–667. doi: 10.1128/jcm.19.5.664-667.1984

Detection of specific immunoglobulin M antibody to different flaviviruses by use of enzyme-labeled antigens.

H Schmitz, P Emmerich
PMCID: PMC271151  PMID: 6330165

Abstract

An enzyme immunoassay was developed for the detection of human immunoglobulin M (IgM) antibody to different flavivirus antigens. The IgM antibody of human sera was selectively bound to anti-IgM antibody-coated solid-phase plates. Flavivirus IgM antibodies were then detected by use of various enzyme-labeled antigens. The flavivirus antigens (dengue type 2 virus, West Nile virus, and tick-borne encephalitis virus) were produced in suckling mice. The antigens were labeled with horseradish peroxidase by adding the activated enzyme at alkaline pH to sucrose-acetone-treated antigens. Addition of unlabeled mouse brain suspension of uninfected animals to the diluted enzyme-labeled antigens effectively reduced nonspecific binding to the solid phase. In patients with acute flavivirus infections, viral IgM antibody could be demonstrated with high sensitivity. Furthermore, the enzyme-labeled antigen-IgM test showed greater specificity than the hemagglutination inhibition test.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burke D. S., Nisalak A. Detection of Japanese encephalitis virus immunoglobulin M antibodies in serum by antibody capture radioimmunoassay. J Clin Microbiol. 1982 Mar;15(3):353–361. doi: 10.1128/jcm.15.3.353-361.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  3. Dittmar D., Cleary T. J., Castro A. Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies. J Clin Microbiol. 1979 Apr;9(4):498–502. doi: 10.1128/jcm.9.4.498-502.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Duermeyer W., van der Veen J. Specific detection of IgM-antibodies by ELISA, applied in hepatitis-A. Lancet. 1978 Sep 23;2(8091):684–685. doi: 10.1016/s0140-6736(78)92802-7. [DOI] [PubMed] [Google Scholar]
  5. Edelman R., Pariyanonda A. Human immunoglobulin M antibody in the sero-diagnosis of Japanese encephalitis virus infections. Am J Epidemiol. 1973 Jul;98(1):29–38. doi: 10.1093/oxfordjournals.aje.a121529. [DOI] [PubMed] [Google Scholar]
  6. Heinz F. X., Roggendorf M., Hofmann H., Kunz C., Deinhardt F. Comparison of two different enzyme immunoassays for detection of immunoglobulin M antibodies against tick-borne encephalitis virus in serum and cerebrospinal fluid. J Clin Microbiol. 1981 Aug;14(2):141–146. doi: 10.1128/jcm.14.2.141-146.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hofmann H., Frisch-Niggemeyer W., Heinz F. Rapid diagnosis of tick-borne encephalitis by means of enzyme linked immunosorbent assay. J Gen Virol. 1979 Mar;42(3):505–511. doi: 10.1099/0022-1317-42-3-505. [DOI] [PubMed] [Google Scholar]
  8. Krech U., Wilhelm J. A. A solid-phase immunosorbent technique for the rapid detection of rubella IgM by haemagglutination inhibition. J Gen Virol. 1979 Aug;44(2):281–286. doi: 10.1099/0022-1317-44-2-281. [DOI] [PubMed] [Google Scholar]
  9. Kunz C., Hofmann H. Due Frühdiagnose der Früsommer-meningoenzephalitis (FSME) im Hämaggflutinationshemmungstest durch Behandlung des Serums mit 2-Mercaptoäthanol. Zentralbl Bakteriol Orig A. 1971 Nov;218(3):273–279. [PubMed] [Google Scholar]
  10. Schmitz H. Detection of immunoglobulin M antibody to Epstein-Barr virus by use of an enzyme-labeled antigen. J Clin Microbiol. 1982 Aug;16(2):361–366. doi: 10.1128/jcm.16.2.361-366.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schmitz H. Improved detection of virus-specific IgM antibodies. Elimination of non-specific IgM binding. J Gen Virol. 1978 Aug;40(2):459–463. doi: 10.1099/0022-1317-40-2-459. [DOI] [PubMed] [Google Scholar]
  12. Schmitz H., Krainick-Rechert C. M. Simple detection of fluorescent stained IgM in sucrose gradients: demonstration of virus-specific IgM. Intervirology. 1974;3(5-6):353–358. doi: 10.1159/000149772. [DOI] [PubMed] [Google Scholar]
  13. Schmitz H., von Deimling U., Flehmig B. Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA). J Gen Virol. 1980 Sep;50(1):59–68. doi: 10.1099/0022-1317-50-1-59. [DOI] [PubMed] [Google Scholar]

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